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Plant essential oils against Aeromonas hydrophila: in vitro activity and their use in experimentally infected fish.
J Appl Microbiol 2015; 119(1):47-54JA

Abstract

AIMS

The aims of this study were to investigate the in vitro antibacterial activity of the essential oils (EOs) of Hesperozygis ringens (HREO), popularly known as 'espanta-pulga' and two different species of basil, Ocimum gratissimum (OGEO) and Ocimum americanum (OAEO), as well as, the potential of these products to be used in silver catfish (Rhamdia quelen) infected with Aeromonas hydrophila.

METHODS AND RESULTS

OGEO and HREO showed better antibacterial activity in vitro. Subinhibitory concentrations of all EOs inhibited haemolysis caused by Aer. hydrophila in fish erythrocytes (100% reduction for OAEO at 100 μg ml(-1) and more than 90% for HREO and OGEO at 150 μg ml(-1)). However, OAEO and HREO showed the best survival results (75 and 70% respectively) after their use as treatment (therapeutic baths-1 h daily/5 days) in silver catfish experimentally infected with Aer. hydrophila. A second in vivo assay using healthy fish was conducted to verify the potential of the EOs (preventive baths-1 h daily/5 days) to promote fish survival. Fish exposed to HREO and OAEO and their diluent (ethanol) showed significant lower haematocrit values and higher complement system activity compared to control. Plasma cortisol level was significantly higher in the groups exposed to both EOs. There was no significant difference in survival of silver catfish challenged with Aer. hydrophila after preventive baths with HREO, OAEO and control group.

CONCLUSIONS

All tested EOs showed in vitro antibacterial properties against Aer. hydrophila and HREO and OAEO showed potential to be used in the treatment of infected fish.

SIGNIFICANCE AND IMPACT OF THE STUDY

These products can be used in aquaculture as therapeutic and prophylactic agents against fish pathogens, with antimicrobial and/or immunostimulant properties.

Authors+Show Affiliations

Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil.Curso de Farmácia, Universidade Regional Integrada do Alto Uruguai e Missões, Santiago, Brazil.Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil.Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, Brazil.Departamento de Zootecnia e Ciências Biológicas/CESNORS, Universidade Federal de Santa Maria, Palmeira das Missões, Brazil.Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, Brazil.Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, Brazil.Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25810355

Citation

Sutili, F J., et al. "Plant Essential Oils Against Aeromonas Hydrophila: in Vitro Activity and Their Use in Experimentally Infected Fish." Journal of Applied Microbiology, vol. 119, no. 1, 2015, pp. 47-54.
Sutili FJ, Silva Lde L, Gressler LT, et al. Plant essential oils against Aeromonas hydrophila: in vitro activity and their use in experimentally infected fish. J Appl Microbiol. 2015;119(1):47-54.
Sutili, F. J., Silva, L. d. e. . L., Gressler, L. T., Gressler, L. T., Battisti, E. K., Heinzmann, B. M., ... Baldisserotto, B. (2015). Plant essential oils against Aeromonas hydrophila: in vitro activity and their use in experimentally infected fish. Journal of Applied Microbiology, 119(1), pp. 47-54. doi:10.1111/jam.12812.
Sutili FJ, et al. Plant Essential Oils Against Aeromonas Hydrophila: in Vitro Activity and Their Use in Experimentally Infected Fish. J Appl Microbiol. 2015;119(1):47-54. PubMed PMID: 25810355.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plant essential oils against Aeromonas hydrophila: in vitro activity and their use in experimentally infected fish. AU - Sutili,F J, AU - Silva,L de Lima, AU - Gressler,L T, AU - Gressler,L T, AU - Battisti,E K, AU - Heinzmann,B M, AU - de Vargas,A C, AU - Baldisserotto,B, Y1 - 2015/04/20/ PY - 2015/02/03/received PY - 2015/03/15/revised PY - 2015/03/16/accepted PY - 2015/3/27/entrez PY - 2015/3/27/pubmed PY - 2016/4/7/medline KW - Hesperozygis ringens KW - Ocimum americanum KW - Ocimum gratissimum KW - Rhamdia quelen KW - cortisol KW - innate immune system SP - 47 EP - 54 JF - Journal of applied microbiology JO - J. Appl. Microbiol. VL - 119 IS - 1 N2 - AIMS: The aims of this study were to investigate the in vitro antibacterial activity of the essential oils (EOs) of Hesperozygis ringens (HREO), popularly known as 'espanta-pulga' and two different species of basil, Ocimum gratissimum (OGEO) and Ocimum americanum (OAEO), as well as, the potential of these products to be used in silver catfish (Rhamdia quelen) infected with Aeromonas hydrophila. METHODS AND RESULTS: OGEO and HREO showed better antibacterial activity in vitro. Subinhibitory concentrations of all EOs inhibited haemolysis caused by Aer. hydrophila in fish erythrocytes (100% reduction for OAEO at 100 μg ml(-1) and more than 90% for HREO and OGEO at 150 μg ml(-1)). However, OAEO and HREO showed the best survival results (75 and 70% respectively) after their use as treatment (therapeutic baths-1 h daily/5 days) in silver catfish experimentally infected with Aer. hydrophila. A second in vivo assay using healthy fish was conducted to verify the potential of the EOs (preventive baths-1 h daily/5 days) to promote fish survival. Fish exposed to HREO and OAEO and their diluent (ethanol) showed significant lower haematocrit values and higher complement system activity compared to control. Plasma cortisol level was significantly higher in the groups exposed to both EOs. There was no significant difference in survival of silver catfish challenged with Aer. hydrophila after preventive baths with HREO, OAEO and control group. CONCLUSIONS: All tested EOs showed in vitro antibacterial properties against Aer. hydrophila and HREO and OAEO showed potential to be used in the treatment of infected fish. SIGNIFICANCE AND IMPACT OF THE STUDY: These products can be used in aquaculture as therapeutic and prophylactic agents against fish pathogens, with antimicrobial and/or immunostimulant properties. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/25810355/Plant_essential_oils_against_Aeromonas_hydrophila:_in_vitro_activity_and_their_use_in_experimentally_infected_fish_ L2 - https://doi.org/10.1111/jam.12812 DB - PRIME DP - Unbound Medicine ER -