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Isolation and molecular characterization of free-living amoebae from different water sources in Italy.
Int J Environ Res Public Health. 2015 Mar 24; 12(4):3417-27.IJ

Abstract

Free-living amoebae (FLA) are protozoa ubiquitous in Nature, isolated from a variety of environments worldwide. In addition to their natural distribution, some species have been found to be pathogenic to humans. In the present study a survey was conducted in order to evaluate the presence and to characterize at molecular level the isolates of amoebic organisms collected from different water sources in Italy. A total of 160 water samples were analyzed by culture and microscopic examination. FLA were found in 46 (28.7%) of the investigated water samples. Groundwater, well waters, and ornamental fountain waters were the sources with higher prevalence rates (85.7%, 50.0%, and 45.9%, respectively). Identification of FLA species/genotypes, based on the 18S rDNA regions, allowed to identify 18 (39.1%) Acanthamoeba isolates (genotypes T4 and T15) and 21 (45.6%) Vermamoeba vermiformis isolates. Other FLA species, including Vahlkampfia sp. and Naegleria spp., previously reported in Italy, were not recovered. The occurrence of potentially pathogenic free-living amoebae in habitats related to human population, as reported in the present study, supports the relevance of FLA as a potential health threat to humans.

Authors+Show Affiliations

Department of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. montalbano.margherita89@gmail.com.Department of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. maristella5384@gmail.com.Interdisciplinary Department of Medicine, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy. piero.lovreglio@uniba.it.Department of Basic Medical Science, Neuroscience and Sense Organ, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy. rosa.monno@uniba.it.Department of Basic Medical Science, Neuroscience and Sense Organ, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy. carla.calia@virgilio.it.Department of Basic Medical Science, Neuroscience and Sense Organ, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy. carla.calia@virgilio.it.Department of Basic Medical Science, Neuroscience and Sense Organ, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy. luciana.fumarola@uniba.it.Department of Systems Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. dalfonso@uniroma2.it.Department of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. berrilli@uniroma2.it.Department of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. dicave@uniroma2.it. Laboratory of Parasitology, Foundation Polyclinic Tor Vergata, Viale Oxford 81, 00133 Rome, Italy. dicave@uniroma2.it.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25811766

Citation

Montalbano Di Filippo, Margherita, et al. "Isolation and Molecular Characterization of Free-living Amoebae From Different Water Sources in Italy." International Journal of Environmental Research and Public Health, vol. 12, no. 4, 2015, pp. 3417-27.
Montalbano Di Filippo M, Santoro M, Lovreglio P, et al. Isolation and molecular characterization of free-living amoebae from different water sources in Italy. Int J Environ Res Public Health. 2015;12(4):3417-27.
Montalbano Di Filippo, M., Santoro, M., Lovreglio, P., Monno, R., Capolongo, C., Calia, C., Fumarola, L., D'Alfonso, R., Berrilli, F., & Di Cave, D. (2015). Isolation and molecular characterization of free-living amoebae from different water sources in Italy. International Journal of Environmental Research and Public Health, 12(4), 3417-27. https://doi.org/10.3390/ijerph120403417
Montalbano Di Filippo M, et al. Isolation and Molecular Characterization of Free-living Amoebae From Different Water Sources in Italy. Int J Environ Res Public Health. 2015 Mar 24;12(4):3417-27. PubMed PMID: 25811766.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and molecular characterization of free-living amoebae from different water sources in Italy. AU - Montalbano Di Filippo,Margherita, AU - Santoro,Maristella, AU - Lovreglio,Piero, AU - Monno,Rosa, AU - Capolongo,Carmen, AU - Calia,Carla, AU - Fumarola,Luciana, AU - D'Alfonso,Rossella, AU - Berrilli,Federica, AU - Di Cave,David, Y1 - 2015/03/24/ PY - 2015/01/19/received PY - 2015/03/13/revised PY - 2015/03/16/accepted PY - 2015/3/27/entrez PY - 2015/3/27/pubmed PY - 2016/5/6/medline SP - 3417 EP - 27 JF - International journal of environmental research and public health JO - Int J Environ Res Public Health VL - 12 IS - 4 N2 - Free-living amoebae (FLA) are protozoa ubiquitous in Nature, isolated from a variety of environments worldwide. In addition to their natural distribution, some species have been found to be pathogenic to humans. In the present study a survey was conducted in order to evaluate the presence and to characterize at molecular level the isolates of amoebic organisms collected from different water sources in Italy. A total of 160 water samples were analyzed by culture and microscopic examination. FLA were found in 46 (28.7%) of the investigated water samples. Groundwater, well waters, and ornamental fountain waters were the sources with higher prevalence rates (85.7%, 50.0%, and 45.9%, respectively). Identification of FLA species/genotypes, based on the 18S rDNA regions, allowed to identify 18 (39.1%) Acanthamoeba isolates (genotypes T4 and T15) and 21 (45.6%) Vermamoeba vermiformis isolates. Other FLA species, including Vahlkampfia sp. and Naegleria spp., previously reported in Italy, were not recovered. The occurrence of potentially pathogenic free-living amoebae in habitats related to human population, as reported in the present study, supports the relevance of FLA as a potential health threat to humans. SN - 1660-4601 UR - https://www.unboundmedicine.com/medline/citation/25811766/Isolation_and_molecular_characterization_of_free_living_amoebae_from_different_water_sources_in_Italy_ L2 - https://www.mdpi.com/resolver?pii=ijerph120403417 DB - PRIME DP - Unbound Medicine ER -