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Detection and preliminary physico-chemical properties of antimicrobial components in the native excretions/secretions of three species of Chrysomya (Diptera, Calliphoridae) in Brazil.
Acta Trop. 2015 Jul; 147:6-11.AT

Abstract

Antibiotic-resistant bacteria in hospitals and communities increasingly threaten public health in Brazil and the rest of the World. There is an urgent need for additional antimicrobial drugs. Calliphorid blowfly larvae are a rich source of antimicrobial factors but the potential of Neotropical species has been neglected. This preliminary study evaluates the antimicrobial activity of the native excretions/secretions of larvae of three species of Brazilian calliphorids, Chrysomya megacephala, Chrysomya albiceps and Chrysomya putoria. Native excretions/secretions were collected from third instar larvae, sterile filtered and tested for antibacterial activity against Staphylococcus aureus 9518, Escherichia coli K12 4401 and Serratia marcescens 365. Turbidometric assays were made in micro-plates, using an ELISA reader, with readings taken up to 22 h. Bacterial suspensions at the start and end of each experiment were also serially diluted, spread on nutrient agar plates and then colony forming units counted. The physico-chemical characteristics of the native excretions/secretions were also tested by freezing/thawing, boiling, and protease digestion. The native excretions/secretions of larvae from these three Chrysomya species significantly inhibited bacterial growth. Therefore, Brazilian calliphorid flies could potentially provide new classes of antibiotics.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ratcliffe NA
Laboratório de Bioquímica e Fisiologia de Insetos, IOC/FIOCRUZ, Rio de Janeiro, RJ, Brazil; Department of Biosciences, College of Science, Swansea University, Singleton Park, Swansea, Wales, UK.
Vieira CS
Laboratório de Bioquímica e Fisiologia de Insetos, IOC/FIOCRUZ, Rio de Janeiro, RJ, Brazil.
Mendonça PM
Laboratório de Transmissores de Leishmanioses, Setor de Entomologia Médica e Forense, IOC/FIOCRUZ, Rio de Janeiro, RJ, Brazil.
Caetano RL
Laboratório de Transmissores de Leishmanioses, Setor de Entomologia Médica e Forense, IOC/FIOCRUZ, Rio de Janeiro, RJ, Brazil.
Queiroz MM
Laboratório de Transmissores de Leishmanioses, Setor de Entomologia Médica e Forense, IOC/FIOCRUZ, Rio de Janeiro, RJ, Brazil.
Garcia ES
Laboratório de Bioquímica e Fisiologia de Insetos, IOC/FIOCRUZ, Rio de Janeiro, RJ, Brazil.
Mello CB
Laboratório de Biologia de Insetos, Departamento de Biologia Geral, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Azambuja P
Laboratório de Bioquímica e Fisiologia de Insetos, IOC/FIOCRUZ, Rio de Janeiro, RJ, Brazil. Electronic address: azambuja.p@gmail.com.

MeSH

AnimalsAnti-Bacterial AgentsAnti-Infective AgentsBodily SecretionsBrazilDipteraDrug DiscoveryEscherichia coli K12LarvaSerratia marcescensStaphylococcus aureus

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25817237

Citation

Ratcliffe, Norman Arthur, et al. "Detection and Preliminary Physico-chemical Properties of Antimicrobial Components in the Native Excretions/secretions of Three Species of Chrysomya (Diptera, Calliphoridae) in Brazil." Acta Tropica, vol. 147, 2015, pp. 6-11.
Ratcliffe NA, Vieira CS, Mendonça PM, et al. Detection and preliminary physico-chemical properties of antimicrobial components in the native excretions/secretions of three species of Chrysomya (Diptera, Calliphoridae) in Brazil. Acta Trop. 2015;147:6-11.
Ratcliffe, N. A., Vieira, C. S., Mendonça, P. M., Caetano, R. L., Queiroz, M. M., Garcia, E. S., Mello, C. B., & Azambuja, P. (2015). Detection and preliminary physico-chemical properties of antimicrobial components in the native excretions/secretions of three species of Chrysomya (Diptera, Calliphoridae) in Brazil. Acta Tropica, 147, 6-11. https://doi.org/10.1016/j.actatropica.2015.03.021
Ratcliffe NA, et al. Detection and Preliminary Physico-chemical Properties of Antimicrobial Components in the Native Excretions/secretions of Three Species of Chrysomya (Diptera, Calliphoridae) in Brazil. Acta Trop. 2015;147:6-11. PubMed PMID: 25817237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and preliminary physico-chemical properties of antimicrobial components in the native excretions/secretions of three species of Chrysomya (Diptera, Calliphoridae) in Brazil. AU - Ratcliffe,Norman Arthur, AU - Vieira,Cecilia Stahl, AU - Mendonça,Paloma Martins, AU - Caetano,Rebecca Leal, AU - Queiroz,Margareth Maria de Carvalho, AU - Garcia,Eloi Souza, AU - Mello,Cicero Brasileiro, AU - Azambuja,Patricia, Y1 - 2015/03/24/ PY - 2014/11/04/received PY - 2015/03/12/revised PY - 2015/03/17/accepted PY - 2015/3/31/entrez PY - 2015/3/31/pubmed PY - 2015/12/31/medline KW - Antibacterial agent KW - Blowflies KW - Chrysomya KW - Escherichia coli KW - Staphylococcus aureus SP - 6 EP - 11 JF - Acta tropica JO - Acta Trop VL - 147 N2 - Antibiotic-resistant bacteria in hospitals and communities increasingly threaten public health in Brazil and the rest of the World. There is an urgent need for additional antimicrobial drugs. Calliphorid blowfly larvae are a rich source of antimicrobial factors but the potential of Neotropical species has been neglected. This preliminary study evaluates the antimicrobial activity of the native excretions/secretions of larvae of three species of Brazilian calliphorids, Chrysomya megacephala, Chrysomya albiceps and Chrysomya putoria. Native excretions/secretions were collected from third instar larvae, sterile filtered and tested for antibacterial activity against Staphylococcus aureus 9518, Escherichia coli K12 4401 and Serratia marcescens 365. Turbidometric assays were made in micro-plates, using an ELISA reader, with readings taken up to 22 h. Bacterial suspensions at the start and end of each experiment were also serially diluted, spread on nutrient agar plates and then colony forming units counted. The physico-chemical characteristics of the native excretions/secretions were also tested by freezing/thawing, boiling, and protease digestion. The native excretions/secretions of larvae from these three Chrysomya species significantly inhibited bacterial growth. Therefore, Brazilian calliphorid flies could potentially provide new classes of antibiotics. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/25817237/Detection_and_preliminary_physico_chemical_properties_of_antimicrobial_components_in_the_native_excretions/secretions_of_three_species_of_Chrysomya__Diptera_Calliphoridae__in_Brazil_ DB - PRIME DP - Unbound Medicine ER -