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MicroRNA-93 regulates collagen loss by targeting MMP3 in human nucleus pulposus cells.
Cell Prolif. 2015 Jun; 48(3):284-92.CP

Abstract

OBJECTIVES

Degenerated disc disease is one of the most common medical conditions in patients suffering from low back pain. Recent studies have shown that microRNAs can regulate cell function in many pathological conditions. The aim of this study was to investigate expression and role of miR-93 in disc degeneration.

MATERIALS AND METHODS

Quantitative RT-PCR was employed to investigate level of miR-93 in degenerative nucleus pulposus (NP) tissues. Then, functional analysis of miR-93 in regulating collagen II expression was performed. Subsequently, western blotting and luciferase reporter assay were used to detect the target gene.

RESULTS

We showed that miR-93 was significantly down-regulated in degenerative NP tissues and its levels were associated with grade of disc degeneration. Overexpression of miR-93 stimulated type II collagen expression in NP cells. Moreover, MMP3 was identified as a putative target of miR-93. MiR-93 inhibited MMP3 expression by directly targeting its 3'UTR, and this was abolished by miR-93 binding site mutations. Additionally, restoration of MMP3 in miR-93-overexpressed NP cells reversed effects of type II collagen expression. Expression of MMP3 inversely correlated with miR-93 expression in degenerative NP tissues.

CONCLUSIONS

Taken together, we demonstrated that miR-93 contributed to abnormal NP cell type II collagen expression by targeting MMP3, involved in intervertebral disc degeneration.

Authors+Show Affiliations

Department of Orthopaedics, Tianjin First Central Hospital, Tianjin, 300192, China.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25818544

Citation

Jing, Wanli, and Wenxue Jiang. "MicroRNA-93 Regulates Collagen Loss By Targeting MMP3 in Human Nucleus Pulposus Cells." Cell Proliferation, vol. 48, no. 3, 2015, pp. 284-92.
Jing W, Jiang W. MicroRNA-93 regulates collagen loss by targeting MMP3 in human nucleus pulposus cells. Cell Prolif. 2015;48(3):284-92.
Jing, W., & Jiang, W. (2015). MicroRNA-93 regulates collagen loss by targeting MMP3 in human nucleus pulposus cells. Cell Proliferation, 48(3), 284-92. https://doi.org/10.1111/cpr.12176
Jing W, Jiang W. MicroRNA-93 Regulates Collagen Loss By Targeting MMP3 in Human Nucleus Pulposus Cells. Cell Prolif. 2015;48(3):284-92. PubMed PMID: 25818544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MicroRNA-93 regulates collagen loss by targeting MMP3 in human nucleus pulposus cells. AU - Jing,Wanli, AU - Jiang,Wenxue, Y1 - 2015/03/27/ PY - 2014/09/24/received PY - 2014/11/27/accepted PY - 2015/3/31/entrez PY - 2015/3/31/pubmed PY - 2015/7/16/medline SP - 284 EP - 92 JF - Cell proliferation JO - Cell Prolif. VL - 48 IS - 3 N2 - OBJECTIVES: Degenerated disc disease is one of the most common medical conditions in patients suffering from low back pain. Recent studies have shown that microRNAs can regulate cell function in many pathological conditions. The aim of this study was to investigate expression and role of miR-93 in disc degeneration. MATERIALS AND METHODS: Quantitative RT-PCR was employed to investigate level of miR-93 in degenerative nucleus pulposus (NP) tissues. Then, functional analysis of miR-93 in regulating collagen II expression was performed. Subsequently, western blotting and luciferase reporter assay were used to detect the target gene. RESULTS: We showed that miR-93 was significantly down-regulated in degenerative NP tissues and its levels were associated with grade of disc degeneration. Overexpression of miR-93 stimulated type II collagen expression in NP cells. Moreover, MMP3 was identified as a putative target of miR-93. MiR-93 inhibited MMP3 expression by directly targeting its 3'UTR, and this was abolished by miR-93 binding site mutations. Additionally, restoration of MMP3 in miR-93-overexpressed NP cells reversed effects of type II collagen expression. Expression of MMP3 inversely correlated with miR-93 expression in degenerative NP tissues. CONCLUSIONS: Taken together, we demonstrated that miR-93 contributed to abnormal NP cell type II collagen expression by targeting MMP3, involved in intervertebral disc degeneration. SN - 1365-2184 UR - https://www.unboundmedicine.com/medline/citation/25818544/MicroRNA_93_regulates_collagen_loss_by_targeting_MMP3_in_human_nucleus_pulposus_cells_ L2 - https://doi.org/10.1111/cpr.12176 DB - PRIME DP - Unbound Medicine ER -