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A myelin basic protein-specific T lymphocyte line that mediates experimental autoimmune encephalomyelitis.
J Immunol. 1985 Jul; 135(1):223-8.JI

Abstract

A T lymphocyte line, BP-1, expressing the T helper phenotype was selected from Lewis rats immunized with guinea pig myelin basic protein (GP-BP) in complete Freund's adjuvant (CFA). The BP-1 line responded specifically to GP-BP but not to PPD after the first round of selection, and responded to rat but not human or bovine BP. When injected i.p. into histocompatible Lewis or F1 (Lewis X P2) recipients, the BP-1 line induced both clinical signs of experimental autoimmune encephalomyelitis (EAE) and delayed type hypersensitivity (DTH) reactions in ears challenged intradermally with GP-BP but not PPD. The severity of clinical signs and the degree of ear swelling were dependent on the dose of BP-1 cells injected. Both activities were detectable with as few as 0.1 X 10(6) BP-1 line cells and required prior activation of the line cells with GP-BP presented by accessory cells. Lewis rats that had recovered from EAE induced by injection of GP-BP in CFA were more susceptible than naive rats to BP-1 line-mediated disease, requiring as few as 0.03 X 10(6) line cells. Clinical EAE and DTH could be serially transferred into F1 (Lewis X P2) recipients with BP-1 cells and back to nonirradiated Lewis parents with activated splenocytes, suggesting that BP-1 cells persist in recipient rats. These results demonstrate the potent biologic activities of an autoreactive BP-specific T lymphocyte line. This line possesses properties similar to BP lines described previously as well as to culture-conditioned splenic T effector cells; thus, the data presented here bridge the gap between these two approaches for studying T effector lymphocyte functions.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

2582032

Citation

Vandenbark, A A., et al. "A Myelin Basic Protein-specific T Lymphocyte Line That Mediates Experimental Autoimmune Encephalomyelitis." Journal of Immunology (Baltimore, Md. : 1950), vol. 135, no. 1, 1985, pp. 223-8.
Vandenbark AA, Gill T, Offner H. A myelin basic protein-specific T lymphocyte line that mediates experimental autoimmune encephalomyelitis. J Immunol. 1985;135(1):223-8.
Vandenbark, A. A., Gill, T., & Offner, H. (1985). A myelin basic protein-specific T lymphocyte line that mediates experimental autoimmune encephalomyelitis. Journal of Immunology (Baltimore, Md. : 1950), 135(1), 223-8.
Vandenbark AA, Gill T, Offner H. A Myelin Basic Protein-specific T Lymphocyte Line That Mediates Experimental Autoimmune Encephalomyelitis. J Immunol. 1985;135(1):223-8. PubMed PMID: 2582032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A myelin basic protein-specific T lymphocyte line that mediates experimental autoimmune encephalomyelitis. AU - Vandenbark,A A, AU - Gill,T, AU - Offner,H, PY - 1985/7/1/pubmed PY - 1985/7/1/medline PY - 1985/7/1/entrez SP - 223 EP - 8 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 135 IS - 1 N2 - A T lymphocyte line, BP-1, expressing the T helper phenotype was selected from Lewis rats immunized with guinea pig myelin basic protein (GP-BP) in complete Freund's adjuvant (CFA). The BP-1 line responded specifically to GP-BP but not to PPD after the first round of selection, and responded to rat but not human or bovine BP. When injected i.p. into histocompatible Lewis or F1 (Lewis X P2) recipients, the BP-1 line induced both clinical signs of experimental autoimmune encephalomyelitis (EAE) and delayed type hypersensitivity (DTH) reactions in ears challenged intradermally with GP-BP but not PPD. The severity of clinical signs and the degree of ear swelling were dependent on the dose of BP-1 cells injected. Both activities were detectable with as few as 0.1 X 10(6) BP-1 line cells and required prior activation of the line cells with GP-BP presented by accessory cells. Lewis rats that had recovered from EAE induced by injection of GP-BP in CFA were more susceptible than naive rats to BP-1 line-mediated disease, requiring as few as 0.03 X 10(6) line cells. Clinical EAE and DTH could be serially transferred into F1 (Lewis X P2) recipients with BP-1 cells and back to nonirradiated Lewis parents with activated splenocytes, suggesting that BP-1 cells persist in recipient rats. These results demonstrate the potent biologic activities of an autoreactive BP-specific T lymphocyte line. This line possesses properties similar to BP lines described previously as well as to culture-conditioned splenic T effector cells; thus, the data presented here bridge the gap between these two approaches for studying T effector lymphocyte functions. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2582032/A_myelin_basic_protein_specific_T_lymphocyte_line_that_mediates_experimental_autoimmune_encephalomyelitis_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2582032 DB - PRIME DP - Unbound Medicine ER -