Tags

Type your tag names separated by a space and hit enter

Growth and development of Colorado potato beetle larvae, Leptinotarsa decemlineata, on potato plants expressing the oryzacystatin II proteinase inhibitor.
Transgenic Res. 2015 Aug; 24(4):729-40.TR

Abstract

Plant proteinase inhibitors (PIs) are attractive tools for crop improvement and their heterologous expression can enhance insect resistance in transgenic plants. PI oryzacystatin II (OCII), isolated from rice, showed potential in controlling pests that utilize cysteine proteinases for protein digestion. To evaluate the applicability of the OCII gene in enhancing plant defence, OCII-transformed potatoes were bioassayed for resistance to Colorado potato beetle (Leptinotarsa decemlineata Say). Feeding on transformed leaves of potato cultivars Desiree and Jelica significantly affected larval growth and development, but did not change mortality rates. During the L2 and L3 developmental stages larvae consumed the OCII-transformed foliage faster as compared to the nontransformed control. Also these larvae reached the prepupal stage (end of L4 stage) 2 days earlier than those fed on control leaves. However, the total amounts of consumed OCII-transformed leaves were up to 23% lower than of control, and the maximal weights of prepupal larvae were reduced by up to 18% as compared to larvae fed on nontransformed leaves. The reduction in insect fitness reported in this study in combination with other control measures, could lead to improved CPB resistance management in potato.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Cingel A
Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar Despota Stefana 142, Belgrade, Serbia, cingel@ibiss.bg.ac.rs.
Savić J
No affiliation info available
Vinterhalter B
No affiliation info available
Vinterhalter D
No affiliation info available
Kostić M
No affiliation info available
Jovanović DŠ
No affiliation info available
Smigocki A
No affiliation info available
Ninković S
No affiliation info available

MeSH

AnimalsColeopteraCystatinsDisease ResistanceGene Expression Regulation, EnzymologicGene Expression Regulation, PlantLarvaPest ControlPlant LeavesPlants, Genetically ModifiedProtease InhibitorsReal-Time Polymerase Chain ReactionSolanum tuberosum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25820664

Citation

Cingel, Aleksandar, et al. "Growth and Development of Colorado Potato Beetle Larvae, Leptinotarsa Decemlineata, On Potato Plants Expressing the Oryzacystatin II Proteinase Inhibitor." Transgenic Research, vol. 24, no. 4, 2015, pp. 729-40.
Cingel A, Savić J, Vinterhalter B, et al. Growth and development of Colorado potato beetle larvae, Leptinotarsa decemlineata, on potato plants expressing the oryzacystatin II proteinase inhibitor. Transgenic Res. 2015;24(4):729-40.
Cingel, A., Savić, J., Vinterhalter, B., Vinterhalter, D., Kostić, M., Jovanović, D. Š., Smigocki, A., & Ninković, S. (2015). Growth and development of Colorado potato beetle larvae, Leptinotarsa decemlineata, on potato plants expressing the oryzacystatin II proteinase inhibitor. Transgenic Research, 24(4), 729-40. https://doi.org/10.1007/s11248-015-9873-9
Cingel A, et al. Growth and Development of Colorado Potato Beetle Larvae, Leptinotarsa Decemlineata, On Potato Plants Expressing the Oryzacystatin II Proteinase Inhibitor. Transgenic Res. 2015;24(4):729-40. PubMed PMID: 25820664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth and development of Colorado potato beetle larvae, Leptinotarsa decemlineata, on potato plants expressing the oryzacystatin II proteinase inhibitor. AU - Cingel,Aleksandar, AU - Savić,Jelena, AU - Vinterhalter,Branka, AU - Vinterhalter,Dragan, AU - Kostić,Miroslav, AU - Jovanović,Darka Šešlija, AU - Smigocki,Ann, AU - Ninković,Slavica, Y1 - 2015/03/29/ PY - 2014/11/21/received PY - 2015/03/23/accepted PY - 2015/3/31/entrez PY - 2015/3/31/pubmed PY - 2016/4/14/medline SP - 729 EP - 40 JF - Transgenic research JO - Transgenic Res VL - 24 IS - 4 N2 - Plant proteinase inhibitors (PIs) are attractive tools for crop improvement and their heterologous expression can enhance insect resistance in transgenic plants. PI oryzacystatin II (OCII), isolated from rice, showed potential in controlling pests that utilize cysteine proteinases for protein digestion. To evaluate the applicability of the OCII gene in enhancing plant defence, OCII-transformed potatoes were bioassayed for resistance to Colorado potato beetle (Leptinotarsa decemlineata Say). Feeding on transformed leaves of potato cultivars Desiree and Jelica significantly affected larval growth and development, but did not change mortality rates. During the L2 and L3 developmental stages larvae consumed the OCII-transformed foliage faster as compared to the nontransformed control. Also these larvae reached the prepupal stage (end of L4 stage) 2 days earlier than those fed on control leaves. However, the total amounts of consumed OCII-transformed leaves were up to 23% lower than of control, and the maximal weights of prepupal larvae were reduced by up to 18% as compared to larvae fed on nontransformed leaves. The reduction in insect fitness reported in this study in combination with other control measures, could lead to improved CPB resistance management in potato. SN - 1573-9368 UR - https://www.unboundmedicine.com/medline/citation/25820664/Growth_and_development_of_Colorado_potato_beetle_larvae_Leptinotarsa_decemlineata_on_potato_plants_expressing_the_oryzacystatin_II_proteinase_inhibitor_ DB - PRIME DP - Unbound Medicine ER -