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Catalpol Modulates Lifespan via DAF-16/FOXO and SKN-1/Nrf2 Activation in Caenorhabditis elegans.

Abstract

Catalpol is an effective component of rehmannia root and known to possess various pharmacological properties. The present study was aimed at investigating the potential effects of catalpol on the lifespan and stress tolerance using C. elegans model system. Herein, catalpol showed potent lifespan extension of wild-type nematode under normal culture condition. In addition, survival rate of catalpol-fed nematodes was significantly elevated compared to untreated control under heat and oxidative stress but not under hyperosmolality conditions. We also found that elevated antioxidant enzyme activities and expressions of stress resistance proteins were attributed to catalpol-mediated increased stress tolerance of nematode. We further investigated whether catalpol's longevity effect is related to aging-related factors including reproduction, food intake, and growth. Interestingly, catalpol exposure could attenuate pharyngeal pumping rate, indicating that catalpol may induce dietary restriction of nematode. Moreover, locomotory ability of aged nematode was significantly improved by catalpol treatment, while lipofuscin levels were attenuated, suggesting that catalpol may affect age-associated changes of nematode. Our mechanistic studies revealed that mek-1, daf-2, age-1, daf-16, and skn-1 are involved in catalpol-mediated longevity. These results indicate that catalpol extends lifespan and increases stress tolerance of C. elegans via DAF-16/FOXO and SKN-1/Nrf activation dependent on insulin/IGF signaling and JNK signaling.

Authors+Show Affiliations

Department of Oriental Pharmacy, College of Pharmacy, Woosuk University, Jeonbuk 565-701, Republic of Korea.Department of Oriental Pharmacy, College of Pharmacy, Woosuk University, Jeonbuk 565-701, Republic of Korea.Department of Medicine, Hematology/Oncology Division, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA.Department of Korean Medical Prescription, College of Korean Medicine, Woosuk University, Jeonbuk 565-701, Republic of Korea.Department of Oriental Pharmacy, College of Pharmacy, Woosuk University, Jeonbuk 565-701, Republic of Korea.Department of Oriental Pharmacy, College of Pharmacy, Woosuk University, Jeonbuk 565-701, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25821490

Citation

Seo, Hyun Won, et al. "Catalpol Modulates Lifespan Via DAF-16/FOXO and SKN-1/Nrf2 Activation in Caenorhabditis Elegans." Evidence-based Complementary and Alternative Medicine : ECAM, vol. 2015, 2015, p. 524878.
Seo HW, Cheon SM, Lee MH, et al. Catalpol Modulates Lifespan via DAF-16/FOXO and SKN-1/Nrf2 Activation in Caenorhabditis elegans. Evid Based Complement Alternat Med. 2015;2015:524878.
Seo, H. W., Cheon, S. M., Lee, M. H., Kim, H. J., Jeon, H., & Cha, D. S. (2015). Catalpol Modulates Lifespan via DAF-16/FOXO and SKN-1/Nrf2 Activation in Caenorhabditis elegans. Evidence-based Complementary and Alternative Medicine : ECAM, 2015, p. 524878. doi:10.1155/2015/524878.
Seo HW, et al. Catalpol Modulates Lifespan Via DAF-16/FOXO and SKN-1/Nrf2 Activation in Caenorhabditis Elegans. Evid Based Complement Alternat Med. 2015;2015:524878. PubMed PMID: 25821490.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Catalpol Modulates Lifespan via DAF-16/FOXO and SKN-1/Nrf2 Activation in Caenorhabditis elegans. AU - Seo,Hyun Won, AU - Cheon,Se Myung, AU - Lee,Myon-Hee, AU - Kim,Hong Jun, AU - Jeon,Hoon, AU - Cha,Dong Seok, Y1 - 2015/03/02/ PY - 2014/11/26/received PY - 2015/01/05/revised PY - 2015/01/09/accepted PY - 2015/3/31/entrez PY - 2015/3/31/pubmed PY - 2015/3/31/medline SP - 524878 EP - 524878 JF - Evidence-based complementary and alternative medicine : eCAM JO - Evid Based Complement Alternat Med VL - 2015 N2 - Catalpol is an effective component of rehmannia root and known to possess various pharmacological properties. The present study was aimed at investigating the potential effects of catalpol on the lifespan and stress tolerance using C. elegans model system. Herein, catalpol showed potent lifespan extension of wild-type nematode under normal culture condition. In addition, survival rate of catalpol-fed nematodes was significantly elevated compared to untreated control under heat and oxidative stress but not under hyperosmolality conditions. We also found that elevated antioxidant enzyme activities and expressions of stress resistance proteins were attributed to catalpol-mediated increased stress tolerance of nematode. We further investigated whether catalpol's longevity effect is related to aging-related factors including reproduction, food intake, and growth. Interestingly, catalpol exposure could attenuate pharyngeal pumping rate, indicating that catalpol may induce dietary restriction of nematode. Moreover, locomotory ability of aged nematode was significantly improved by catalpol treatment, while lipofuscin levels were attenuated, suggesting that catalpol may affect age-associated changes of nematode. Our mechanistic studies revealed that mek-1, daf-2, age-1, daf-16, and skn-1 are involved in catalpol-mediated longevity. These results indicate that catalpol extends lifespan and increases stress tolerance of C. elegans via DAF-16/FOXO and SKN-1/Nrf activation dependent on insulin/IGF signaling and JNK signaling. SN - 1741-427X UR - https://www.unboundmedicine.com/medline/citation/25821490/Catalpol_Modulates_Lifespan_via_DAF_16/FOXO_and_SKN_1/Nrf2_Activation_in_Caenorhabditis_elegans_ L2 - https://dx.doi.org/10.1155/2015/524878 DB - PRIME DP - Unbound Medicine ER -