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The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis elegans but has No Effect on Life Span.
Phytother Res 2015; 29(6):894-901PR

Abstract

The lignan pinoresinol is a constituent of flaxseed, sesame seeds and olive oil. Because of different molecular effects reported for this compound, e.g. antioxidative activity, pinoresinol is suggested to cause positive effects on humans. Because experimental data are limited, we have analysed the effects of the lignan on the nematode Caenorhabditis elegans: in spite of a strong antioxidative capacity detected in an in vitro assay, no antioxidative effects were detectable in vivo. In analogy to this result, no modulation of the sensitivity against thermal stress was detectable. However, incubation with pinoresinol caused an enhanced nuclear accumulation of the transcription factor DAF-16 (insulin/IGF-like signalling pathway). Using a strain with an enhanced oxidative stress level (mev-1 mutant), we clearly see an increase in stress resistance caused by this lignan, but no change in reactive oxygen species. Furthermore, we investigated the effects of pinoresinol on the life span of the nematode, but no modulation was found, neither in wild-type nor in mev-1 mutant nematodes. These results suggest that pinoresinol may exert pharmacologically interesting effects via modulation of the insulin-like signalling pathway in C. elegans as well as in other species like mammals due to the evolutionary conservation of this signalling pathway.

Authors+Show Affiliations

Martin Luther University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Biofunctionality of Secondary Plant Compounds, Weinbergweg 22 (Biozentrum), 06120, Halle/Saale, Germany.Martin Luther University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Biofunctionality of Secondary Plant Compounds, Weinbergweg 22 (Biozentrum), 06120, Halle/Saale, Germany.Martin Luther University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Biofunctionality of Secondary Plant Compounds, Weinbergweg 22 (Biozentrum), 06120, Halle/Saale, Germany.Martin Luther University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Biofunctionality of Secondary Plant Compounds, Weinbergweg 22 (Biozentrum), 06120, Halle/Saale, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25826281

Citation

Koch, Karoline, et al. "The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis Elegans but Has No Effect On Life Span." Phytotherapy Research : PTR, vol. 29, no. 6, 2015, pp. 894-901.
Koch K, Büchter C, Havermann S, et al. The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis elegans but has No Effect on Life Span. Phytother Res. 2015;29(6):894-901.
Koch, K., Büchter, C., Havermann, S., & Wätjen, W. (2015). The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis elegans but has No Effect on Life Span. Phytotherapy Research : PTR, 29(6), pp. 894-901. doi:10.1002/ptr.5330.
Koch K, et al. The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis Elegans but Has No Effect On Life Span. Phytother Res. 2015;29(6):894-901. PubMed PMID: 25826281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis elegans but has No Effect on Life Span. AU - Koch,Karoline, AU - Büchter,Christian, AU - Havermann,Susannah, AU - Wätjen,Wim, Y1 - 2015/03/31/ PY - 2014/08/13/received PY - 2015/02/23/revised PY - 2015/02/25/accepted PY - 2015/4/1/entrez PY - 2015/4/1/pubmed PY - 2015/10/7/medline KW - DAF-16 KW - ageing KW - lignan KW - oxidative stress KW - pinoresinol KW - secondary plant compounds SP - 894 EP - 901 JF - Phytotherapy research : PTR JO - Phytother Res VL - 29 IS - 6 N2 - The lignan pinoresinol is a constituent of flaxseed, sesame seeds and olive oil. Because of different molecular effects reported for this compound, e.g. antioxidative activity, pinoresinol is suggested to cause positive effects on humans. Because experimental data are limited, we have analysed the effects of the lignan on the nematode Caenorhabditis elegans: in spite of a strong antioxidative capacity detected in an in vitro assay, no antioxidative effects were detectable in vivo. In analogy to this result, no modulation of the sensitivity against thermal stress was detectable. However, incubation with pinoresinol caused an enhanced nuclear accumulation of the transcription factor DAF-16 (insulin/IGF-like signalling pathway). Using a strain with an enhanced oxidative stress level (mev-1 mutant), we clearly see an increase in stress resistance caused by this lignan, but no change in reactive oxygen species. Furthermore, we investigated the effects of pinoresinol on the life span of the nematode, but no modulation was found, neither in wild-type nor in mev-1 mutant nematodes. These results suggest that pinoresinol may exert pharmacologically interesting effects via modulation of the insulin-like signalling pathway in C. elegans as well as in other species like mammals due to the evolutionary conservation of this signalling pathway. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/25826281/The_Lignan_Pinoresinol_Induces_Nuclear_Translocation_of_DAF_16_in_Caenorhabditis_elegans_but_has_No_Effect_on_Life_Span_ L2 - https://doi.org/10.1002/ptr.5330 DB - PRIME DP - Unbound Medicine ER -