Tags

Type your tag names separated by a space and hit enter

Development and validation of a new STR 25-plex typing system.
Forensic Sci Int Genet. 2015 Jul; 17:61-69.FS

Abstract

In this study, a new STR 25-plex typing system, including 23 autosomal STRs (D1S1656, D2S1338, D2S441, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, CSF1PO, FGA, Penta D, Penta E, TH01, TPOX, vWA) and a Y-STR locus of DYS391 and amelogenin, was developed. The included 24 STRs belonged to the main international DNA databases (CODIS, ISSL, ESS-extended, UCL, GCL and NCIDD) except D6S1043 (specially chosen for Chinese population). Developmental validation indicated that the STR 25-plex typing system was reproducible, accurate, sensitive and robust. The sensitivity testing of the system was such that a full profile was obtainable even with 125pg of human DNA. Specificity testing was demonstrated by the lack of cross-reactivity with a variety of commonly encountered animal species and microbial pool. For the stability testing, full profiles can been obtained with humic acid concentration≤60ng/μL and hematin<500μM. Also, this multiplex system is suitable for mixture study. All of the minor alleles were called for ratios of 1:1, 1:3 and 3:1 of the mixture with the system. In addition, the whole PCR amplification can finish within 1h, making the system suitable for fast-detection. For the forensic evaluation of the multiplex system, 23 autosomal STRs included followed the Hardy-Weinberg equilibrium. A total of 268 alleles were detected for the 23 autosomal STR loci among 200 individuals. Since 23 autosomal STRs were independent from each other, CMECduo was 0.99999916563607 and CMECtri was 0.99999999986525. All the forensic efficiency parameters demonstrated that this multiplex system is highly polymorphic and informative in the Han population of China.

Authors+Show Affiliations

Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China; State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China. Electronic address: zsh-daisy@163.com.Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China.PEOPLESPOTINC R&D, Beijing 102206, PR China.Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China.Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China; Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, PR China.Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China. Electronic address: lichengtaohla@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

25828368

Citation

Zhang, Suhua, et al. "Development and Validation of a New STR 25-plex Typing System." Forensic Science International. Genetics, vol. 17, 2015, pp. 61-69.
Zhang S, Bian Y, Tian H, et al. Development and validation of a new STR 25-plex typing system. Forensic Sci Int Genet. 2015;17:61-69.
Zhang, S., Bian, Y., Tian, H., Wang, Z., Hu, Z., & Li, C. (2015). Development and validation of a new STR 25-plex typing system. Forensic Science International. Genetics, 17, 61-69. https://doi.org/10.1016/j.fsigen.2015.03.008
Zhang S, et al. Development and Validation of a New STR 25-plex Typing System. Forensic Sci Int Genet. 2015;17:61-69. PubMed PMID: 25828368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of a new STR 25-plex typing system. AU - Zhang,Suhua, AU - Bian,Yingnan, AU - Tian,Huaizhou, AU - Wang,Zheng, AU - Hu,Zhen, AU - Li,Chengtao, Y1 - 2015/03/14/ PY - 2014/09/29/received PY - 2015/01/19/revised PY - 2015/03/10/accepted PY - 2015/4/2/entrez PY - 2015/4/2/pubmed PY - 2016/4/19/medline KW - DNA typing KW - Forensic science KW - Multiplex PCR KW - Short tandem repeat (STR) KW - Validation SP - 61 EP - 69 JF - Forensic science international. Genetics JO - Forensic Sci Int Genet VL - 17 N2 - In this study, a new STR 25-plex typing system, including 23 autosomal STRs (D1S1656, D2S1338, D2S441, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, CSF1PO, FGA, Penta D, Penta E, TH01, TPOX, vWA) and a Y-STR locus of DYS391 and amelogenin, was developed. The included 24 STRs belonged to the main international DNA databases (CODIS, ISSL, ESS-extended, UCL, GCL and NCIDD) except D6S1043 (specially chosen for Chinese population). Developmental validation indicated that the STR 25-plex typing system was reproducible, accurate, sensitive and robust. The sensitivity testing of the system was such that a full profile was obtainable even with 125pg of human DNA. Specificity testing was demonstrated by the lack of cross-reactivity with a variety of commonly encountered animal species and microbial pool. For the stability testing, full profiles can been obtained with humic acid concentration≤60ng/μL and hematin<500μM. Also, this multiplex system is suitable for mixture study. All of the minor alleles were called for ratios of 1:1, 1:3 and 3:1 of the mixture with the system. In addition, the whole PCR amplification can finish within 1h, making the system suitable for fast-detection. For the forensic evaluation of the multiplex system, 23 autosomal STRs included followed the Hardy-Weinberg equilibrium. A total of 268 alleles were detected for the 23 autosomal STR loci among 200 individuals. Since 23 autosomal STRs were independent from each other, CMECduo was 0.99999916563607 and CMECtri was 0.99999999986525. All the forensic efficiency parameters demonstrated that this multiplex system is highly polymorphic and informative in the Han population of China. SN - 1878-0326 UR - https://www.unboundmedicine.com/medline/citation/25828368/Development_and_validation_of_a_new_STR_25_plex_typing_system_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1872-4973(15)00045-9 DB - PRIME DP - Unbound Medicine ER -