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Molecular cloning and tissue expression of uncoupling protein 1, 2 and 3 genes in Chinese perch (Siniperca chuatsi).

Abstract

Uncoupling proteins (UCPs) are mitochondrial anion carrier proteins, which play important roles in several physiological processes, including thermogenesis, reactive oxygen species generation, growth, lipid metabolism and insulin secretion. Although the roles of UCPs are well understood in mammals, little is known in fish. To investigate the thermogenesis roles in Chinese perch (Siniperca chuatsi), we cloned the UCP1, 2 and 3. The UCP1 consisted of six exons and five introns, and the UCP2 consisted of eight exons and seven introns. The UCP1 was primarily expressed in liver, UCP2 was ubiquitously expressed, and UCP3 was primarily expressed in muscle. The mRNA levels of UCP1 and UCP2 in liver, and UCP3 in muscle were significantly increased after prolonged cold exposure, but did not change after prolonged heat exposure, suggesting that Chinese perch might have a mechanism of response to cold environment, but not to hot environment. The intestinal UCP1 mRNA level was significantly up-regulated after prolonged heat exposure, while the UCP2 mRNA level was significantly up-regulated after prolonged cold exposure, suggesting that the two paralogs might play different roles in intestine of Chinese perch. In addition, the phylogenetic analysis could shed new light on the evolutionary diversification of UCP gene family.

Authors+Show Affiliations

College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China. Electronic address: xufang_liang@yahoo.com.College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.Department of Anatomy, Physiology, and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849-5519, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25829150

Citation

Wen, Zheng-Yong, et al. "Molecular Cloning and Tissue Expression of Uncoupling Protein 1, 2 and 3 Genes in Chinese Perch (Siniperca Chuatsi)." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 185, 2015, pp. 24-33.
Wen ZY, Liang XF, He S, et al. Molecular cloning and tissue expression of uncoupling protein 1, 2 and 3 genes in Chinese perch (Siniperca chuatsi). Comp Biochem Physiol B Biochem Mol Biol. 2015;185:24-33.
Wen, Z. Y., Liang, X. F., He, S., Li, L., Shen, D., & Tao, Y. X. (2015). Molecular cloning and tissue expression of uncoupling protein 1, 2 and 3 genes in Chinese perch (Siniperca chuatsi). Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 185, 24-33. https://doi.org/10.1016/j.cbpb.2015.03.005
Wen ZY, et al. Molecular Cloning and Tissue Expression of Uncoupling Protein 1, 2 and 3 Genes in Chinese Perch (Siniperca Chuatsi). Comp Biochem Physiol B Biochem Mol Biol. 2015;185:24-33. PubMed PMID: 25829150.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and tissue expression of uncoupling protein 1, 2 and 3 genes in Chinese perch (Siniperca chuatsi). AU - Wen,Zheng-Yong, AU - Liang,Xu-Fang, AU - He,Shan, AU - Li,Ling, AU - Shen,Dan, AU - Tao,Ya-Xiong, Y1 - 2015/03/28/ PY - 2014/11/17/received PY - 2015/03/17/revised PY - 2015/03/22/accepted PY - 2015/4/2/entrez PY - 2015/4/2/pubmed PY - 2016/1/27/medline KW - Gene expression KW - Gene structures KW - Thermogenesis KW - Uncoupling protein SP - 24 EP - 33 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp Biochem Physiol B Biochem Mol Biol VL - 185 N2 - Uncoupling proteins (UCPs) are mitochondrial anion carrier proteins, which play important roles in several physiological processes, including thermogenesis, reactive oxygen species generation, growth, lipid metabolism and insulin secretion. Although the roles of UCPs are well understood in mammals, little is known in fish. To investigate the thermogenesis roles in Chinese perch (Siniperca chuatsi), we cloned the UCP1, 2 and 3. The UCP1 consisted of six exons and five introns, and the UCP2 consisted of eight exons and seven introns. The UCP1 was primarily expressed in liver, UCP2 was ubiquitously expressed, and UCP3 was primarily expressed in muscle. The mRNA levels of UCP1 and UCP2 in liver, and UCP3 in muscle were significantly increased after prolonged cold exposure, but did not change after prolonged heat exposure, suggesting that Chinese perch might have a mechanism of response to cold environment, but not to hot environment. The intestinal UCP1 mRNA level was significantly up-regulated after prolonged heat exposure, while the UCP2 mRNA level was significantly up-regulated after prolonged cold exposure, suggesting that the two paralogs might play different roles in intestine of Chinese perch. In addition, the phylogenetic analysis could shed new light on the evolutionary diversification of UCP gene family. SN - 1879-1107 UR - https://www.unboundmedicine.com/medline/citation/25829150/Molecular_cloning_and_tissue_expression_of_uncoupling_protein_1_2_and_3_genes_in_Chinese_perch__Siniperca_chuatsi__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096-4959(15)00047-0 DB - PRIME DP - Unbound Medicine ER -