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Vitexin protects brain against ischemia/reperfusion injury via modulating mitogen-activated protein kinase and apoptosis signaling in mice.
Phytomedicine. 2015 Mar 15; 22(3):379-84.P

Abstract

Vitexin is a major bioactive flavonoid compound derived from the dried leaf of hawthorn (Crataegus pinnatifida), a widely used conventional folk medicine in China. Recent studies have shown that vitexin presents neuroprotective effects in vitro. Whether this protective effect applies to the cerebral ischemia/reperfusion (I/R) injury remains elusive. In the present study, we examined the potential neuroprotective effect of vitexin against cerebral I/R injury and underlying mechanisms. A focal cerebral I/R model in male Kunming mice was induced by middle cerebral artery occlusion (MCAO) for 2 h followed by reperfusion for 22 h. The neurological function and infarct volume were assessed by using Long's five-point scale system and triphenyl-tetrazolium chloride (TTC) staining technique, respectively. Neuronal damage was evaluated by histological staining. Extracellular signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinases (JNK) and p38 phosphorylation, and apoptosis were measured via Western blot at 24 h after reperfusion. As a result, systemic vitexin treatment significantly reduced neurological deficit, cerebral infarct volume and neuronal damage when compared with the I/R group. Western blot analyses revealed that vitexin markedly upregulated p-ERK1/2 and downregulated p-JNK and p-p38. Meanwhile, vitexin increased Bcl-2 expression and suppressed the overexpression of Bax in the I/R injury mice. In conclusion, the results indicate that vitexin protects brain against cerebral I/R injury, and this effect may be regulated by mitogen-activated protein kinase (MAPK) and apoptosis signaling pathways.

Authors+Show Affiliations

Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China.Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China.Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China.Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China.Hefei Qi-xing Medicine and Technology Co. Ltd, Hefei, Anhui 230032, China.Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China.Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China. Electronic address: gongliangzhang@hotmail.com.Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China. Electronic address: dongly@ahmu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25837275

Citation

Wang, Yanan, et al. "Vitexin Protects Brain Against Ischemia/reperfusion Injury Via Modulating Mitogen-activated Protein Kinase and Apoptosis Signaling in Mice." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 22, no. 3, 2015, pp. 379-84.
Wang Y, Zhen Y, Wu X, et al. Vitexin protects brain against ischemia/reperfusion injury via modulating mitogen-activated protein kinase and apoptosis signaling in mice. Phytomedicine. 2015;22(3):379-84.
Wang, Y., Zhen, Y., Wu, X., Jiang, Q., Li, X., Chen, Z., Zhang, G., & Dong, L. (2015). Vitexin protects brain against ischemia/reperfusion injury via modulating mitogen-activated protein kinase and apoptosis signaling in mice. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 22(3), 379-84. https://doi.org/10.1016/j.phymed.2015.01.009
Wang Y, et al. Vitexin Protects Brain Against Ischemia/reperfusion Injury Via Modulating Mitogen-activated Protein Kinase and Apoptosis Signaling in Mice. Phytomedicine. 2015 Mar 15;22(3):379-84. PubMed PMID: 25837275.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitexin protects brain against ischemia/reperfusion injury via modulating mitogen-activated protein kinase and apoptosis signaling in mice. AU - Wang,Yanan, AU - Zhen,Yilan, AU - Wu,Xian, AU - Jiang,Qin, AU - Li,Xiaoliang, AU - Chen,Zhiwu, AU - Zhang,Gongliang, AU - Dong,Liuyi, Y1 - 2015/02/23/ PY - 2014/07/27/received PY - 2015/01/26/revised PY - 2015/01/29/accepted PY - 2015/4/4/entrez PY - 2015/4/4/pubmed PY - 2015/9/30/medline KW - Apoptosis KW - Ischemia/reperfusion injury KW - Mice KW - Mitogen-activated protein kinase KW - Vitexin SP - 379 EP - 84 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 22 IS - 3 N2 - Vitexin is a major bioactive flavonoid compound derived from the dried leaf of hawthorn (Crataegus pinnatifida), a widely used conventional folk medicine in China. Recent studies have shown that vitexin presents neuroprotective effects in vitro. Whether this protective effect applies to the cerebral ischemia/reperfusion (I/R) injury remains elusive. In the present study, we examined the potential neuroprotective effect of vitexin against cerebral I/R injury and underlying mechanisms. A focal cerebral I/R model in male Kunming mice was induced by middle cerebral artery occlusion (MCAO) for 2 h followed by reperfusion for 22 h. The neurological function and infarct volume were assessed by using Long's five-point scale system and triphenyl-tetrazolium chloride (TTC) staining technique, respectively. Neuronal damage was evaluated by histological staining. Extracellular signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinases (JNK) and p38 phosphorylation, and apoptosis were measured via Western blot at 24 h after reperfusion. As a result, systemic vitexin treatment significantly reduced neurological deficit, cerebral infarct volume and neuronal damage when compared with the I/R group. Western blot analyses revealed that vitexin markedly upregulated p-ERK1/2 and downregulated p-JNK and p-p38. Meanwhile, vitexin increased Bcl-2 expression and suppressed the overexpression of Bax in the I/R injury mice. In conclusion, the results indicate that vitexin protects brain against cerebral I/R injury, and this effect may be regulated by mitogen-activated protein kinase (MAPK) and apoptosis signaling pathways. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/25837275/Vitexin_protects_brain_against_ischemia/reperfusion_injury_via_modulating_mitogen_activated_protein_kinase_and_apoptosis_signaling_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(15)00032-X DB - PRIME DP - Unbound Medicine ER -