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Modulatory effect of Senecio brasiliensis (Spreng) Less. in a murine model of inflammation induced by carrageenan into the pleural cavity.
J Ethnopharmacol. 2015 Jun 20; 168:373-9.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Senecio brasiliensis (Spreng) Less (S. brasiliensis), known as "Flor-das-almas", "Margaridinha" or "Maria mole", is used in folk medicine as an anti-inflammatory and to treat gastric ulcers and stomach pain. While the Senecio genus has been widely studied for its pharmacological activities to support its use in traditional medicine, few studies focus on the anti-inflammatory activities of the species.

AIM OF THE STUDY

To investigate the anti-inflammatory activities of S. brasiliensis, a specie native to Brazil, using a murine model of pleurisy induced by carrageenan.

MATERIAL AND METHODS

The flowers of S. brasiliensis were air-dried for 3 days and subjected to ethanol (96%) extraction for 7 days to obtain the crude extract (CE). The CE was subjected to acid-base extraction to obtain the alkaloid fraction (AF). The hexane (HEX), dichloromethane (DCM) and ethyl acetate (EtOAc) fractions were obtained by extracting from CE with different solvents. The alkaloids senecionine (Sen), integerrimine (Int) and senecionine N-oxide were obtained from AF by chromatographic fractionation and a mixture of 1,4-, 3,4-, 3,5- and 4,5-dicaffeoylquinic acids (DCQs) were obtained from the EtOAc fraction. The isolated alkaloids were identified through spectroscopic analysis of IR, NMR and LC-MS coupled with electrospray ionization mass spectrometry (ESI-MS), and the dicaffeoylquinic acids through the hierarchical key method. Swiss mice were used in the in vivo experiments. We evaluated the effect of the CE, its derived fractions (AF, HEX, DCM and EtOAc), and the isolated compounds (Sen, Int, N-oxide senecionine, and DCQs) on: leukocyte migration, exudate concentrations, myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities, and tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) and interleukin 17A levels in the fluid leakage from the pleural cavity using a mouse model of pleurisy induced by carrageenan. The effects of the isolated compounds, Sen, Int, N-oxide senecionine and DCQs, were also analyzed for their ability to inhibit p65 phosphorylation (p-p65) in the nuclear factor-kappa B (NF-κB) pathway in the lung tissue. MPO and ADA were analyzed by colorimetric assays, and the cytokines and protein p65 levels were determined using an enzyme immunoassay (EIA).

RESULTS

The CE, its EtOAc and AF fractions, and its isolated compounds (Sen, Int and DCQs), significantly reduced leukocyte migration (P < 0.05), MPO and ADA activities (P < 0.01), and TNF-α (P < 0.05), and IL-17A levels (P < 0.01). The CE, the EtOAc and AF fractions, and the DCQs also decreased IL-1β levels (P < 0.01). The isolated compounds, Sen, Int and the DCQs, inhibited p65 phosphorylation (NF-κB) (P < 0.05).

CONCLUSION

This study demonstrated that S. brasiliensis has important anti-inflammatory properties that are capable of inhibiting activated leukocytes by decreasing neutrophil migration. This effect may be attributed to the inhibition of pro-inflammatory cytokines and the reduction of the NF-κB pathway. The compounds Sen, Int, and DCQs may be responsible for the anti-inflammatory actions of S. brasiliensis.

Authors+Show Affiliations

Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis 88040-970, SC, Brazil.Department of Chemistry, Center of Physical and Mathematical Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis 88040-900, SC, Brazil.Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis 88040-970, SC, Brazil.Department of Chemistry, Center of Physical and Mathematical Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis 88040-900, SC, Brazil.Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis 88040-970, SC, Brazil. Electronic address: tania.frode@ufsc.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25839117

Citation

de Souza, Renata Rodrigues, et al. "Modulatory Effect of Senecio Brasiliensis (Spreng) Less. in a Murine Model of Inflammation Induced By Carrageenan Into the Pleural Cavity." Journal of Ethnopharmacology, vol. 168, 2015, pp. 373-9.
de Souza RR, Bretanha LC, Dalmarco EM, et al. Modulatory effect of Senecio brasiliensis (Spreng) Less. in a murine model of inflammation induced by carrageenan into the pleural cavity. J Ethnopharmacol. 2015;168:373-9.
de Souza, R. R., Bretanha, L. C., Dalmarco, E. M., Pizzolatti, M. G., & Fröde, T. S. (2015). Modulatory effect of Senecio brasiliensis (Spreng) Less. in a murine model of inflammation induced by carrageenan into the pleural cavity. Journal of Ethnopharmacology, 168, 373-9. https://doi.org/10.1016/j.jep.2015.03.032
de Souza RR, et al. Modulatory Effect of Senecio Brasiliensis (Spreng) Less. in a Murine Model of Inflammation Induced By Carrageenan Into the Pleural Cavity. J Ethnopharmacol. 2015 Jun 20;168:373-9. PubMed PMID: 25839117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulatory effect of Senecio brasiliensis (Spreng) Less. in a murine model of inflammation induced by carrageenan into the pleural cavity. AU - de Souza,Renata Rodrigues, AU - Bretanha,Lizandra Czermainski, AU - Dalmarco,Eduardo Monguilhott, AU - Pizzolatti,Moacir Geraldo, AU - Fröde,Tânia Silvia, Y1 - 2015/03/31/ PY - 2015/01/22/received PY - 2015/02/20/revised PY - 2015/03/02/accepted PY - 2015/4/4/entrez PY - 2015/4/4/pubmed PY - 2016/3/22/medline KW - Anti-inflammatory effect KW - Cytokines KW - Dicaffeoylquinic acid KW - NF-Kappa B pathway KW - Pyrrolizidine alkaloids KW - Senecio brasiliensis SP - 373 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 168 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Senecio brasiliensis (Spreng) Less (S. brasiliensis), known as "Flor-das-almas", "Margaridinha" or "Maria mole", is used in folk medicine as an anti-inflammatory and to treat gastric ulcers and stomach pain. While the Senecio genus has been widely studied for its pharmacological activities to support its use in traditional medicine, few studies focus on the anti-inflammatory activities of the species. AIM OF THE STUDY: To investigate the anti-inflammatory activities of S. brasiliensis, a specie native to Brazil, using a murine model of pleurisy induced by carrageenan. MATERIAL AND METHODS: The flowers of S. brasiliensis were air-dried for 3 days and subjected to ethanol (96%) extraction for 7 days to obtain the crude extract (CE). The CE was subjected to acid-base extraction to obtain the alkaloid fraction (AF). The hexane (HEX), dichloromethane (DCM) and ethyl acetate (EtOAc) fractions were obtained by extracting from CE with different solvents. The alkaloids senecionine (Sen), integerrimine (Int) and senecionine N-oxide were obtained from AF by chromatographic fractionation and a mixture of 1,4-, 3,4-, 3,5- and 4,5-dicaffeoylquinic acids (DCQs) were obtained from the EtOAc fraction. The isolated alkaloids were identified through spectroscopic analysis of IR, NMR and LC-MS coupled with electrospray ionization mass spectrometry (ESI-MS), and the dicaffeoylquinic acids through the hierarchical key method. Swiss mice were used in the in vivo experiments. We evaluated the effect of the CE, its derived fractions (AF, HEX, DCM and EtOAc), and the isolated compounds (Sen, Int, N-oxide senecionine, and DCQs) on: leukocyte migration, exudate concentrations, myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities, and tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) and interleukin 17A levels in the fluid leakage from the pleural cavity using a mouse model of pleurisy induced by carrageenan. The effects of the isolated compounds, Sen, Int, N-oxide senecionine and DCQs, were also analyzed for their ability to inhibit p65 phosphorylation (p-p65) in the nuclear factor-kappa B (NF-κB) pathway in the lung tissue. MPO and ADA were analyzed by colorimetric assays, and the cytokines and protein p65 levels were determined using an enzyme immunoassay (EIA). RESULTS: The CE, its EtOAc and AF fractions, and its isolated compounds (Sen, Int and DCQs), significantly reduced leukocyte migration (P < 0.05), MPO and ADA activities (P < 0.01), and TNF-α (P < 0.05), and IL-17A levels (P < 0.01). The CE, the EtOAc and AF fractions, and the DCQs also decreased IL-1β levels (P < 0.01). The isolated compounds, Sen, Int and the DCQs, inhibited p65 phosphorylation (NF-κB) (P < 0.05). CONCLUSION: This study demonstrated that S. brasiliensis has important anti-inflammatory properties that are capable of inhibiting activated leukocytes by decreasing neutrophil migration. This effect may be attributed to the inhibition of pro-inflammatory cytokines and the reduction of the NF-κB pathway. The compounds Sen, Int, and DCQs may be responsible for the anti-inflammatory actions of S. brasiliensis. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/25839117/Modulatory_effect_of_Senecio_brasiliensis__Spreng__Less__in_a_murine_model_of_inflammation_induced_by_carrageenan_into_the_pleural_cavity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)00176-2 DB - PRIME DP - Unbound Medicine ER -