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A novel polysaccharide from mycelia of cultured Phellinus linteus displays antitumor activity through apoptosis.
Carbohydr Polym. 2015 Jun 25; 124:90-7.CP

Abstract

Two novel polysaccharides termed PLPS-1 and PLPS-2 were isolated from mycelia of cultured Phellinus linteus by hot water extraction, purified by DEAE-52 cellulose and Sephadex G-100 column chromatography, and structurally characterized by FTIR and NMR spectroscopy, GC-MS, periodate oxidation/Smith degradation, and methylation analysis. The monosaccharide compositions of PLPS-1 (MW 2.5×10(5)Da) and PLPS-2 (MW 2.8×10(4)Da) were respectively Glc, Ara, Fuc, Gal, and Xyl in molar ratio 21.964:1.336:1.182:1:1, and Glc, Gal, Man, Ara, Fuc, Xyl in molar ratio 14.368:2.594:1.956:1.552:1.466:1; i.e., both were heteropolysaccharides. The backbone of PLPS-1 consisted primarily of repeating α-d-Glc(1→4)-α-d-Glc(1→6) units, while that of PLPS-2 consisted of α-(1→3)-d-Glc and α-(1→6)-d-Glc. The side branches were also different in their carbohydrate components. In in vitro antitumor assays, PLPS-1 displayed strong anti-proliferative effect against S-180 sarcoma cells through apoptosis, whereas PLPS-2 had no such effect. The difference in antitumor activity between the two PLPS evidently results from their structural differences. PLPS-1 has potential as a novel anticancer agent.

Authors+Show Affiliations

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: lyxhzau@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25839798

Citation

Mei, Yuxia, et al. "A Novel Polysaccharide From Mycelia of Cultured Phellinus Linteus Displays Antitumor Activity Through Apoptosis." Carbohydrate Polymers, vol. 124, 2015, pp. 90-7.
Mei Y, Zhu H, Hu Q, et al. A novel polysaccharide from mycelia of cultured Phellinus linteus displays antitumor activity through apoptosis. Carbohydr Polym. 2015;124:90-7.
Mei, Y., Zhu, H., Hu, Q., Liu, Y., Zhao, S., Peng, N., & Liang, Y. (2015). A novel polysaccharide from mycelia of cultured Phellinus linteus displays antitumor activity through apoptosis. Carbohydrate Polymers, 124, 90-7. https://doi.org/10.1016/j.carbpol.2015.02.009
Mei Y, et al. A Novel Polysaccharide From Mycelia of Cultured Phellinus Linteus Displays Antitumor Activity Through Apoptosis. Carbohydr Polym. 2015 Jun 25;124:90-7. PubMed PMID: 25839798.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel polysaccharide from mycelia of cultured Phellinus linteus displays antitumor activity through apoptosis. AU - Mei,Yuxia, AU - Zhu,Hai, AU - Hu,Qiming, AU - Liu,Yangyang, AU - Zhao,Shumiao, AU - Peng,Nan, AU - Liang,Yunxiang, Y1 - 2015/02/14/ PY - 2014/09/22/received PY - 2015/01/30/revised PY - 2015/02/08/accepted PY - 2015/4/4/entrez PY - 2015/4/4/pubmed PY - 2015/12/17/medline KW - Antitumor activity KW - Apoptosis KW - Phellinus linteus KW - Polysaccharides KW - Structural analysis SP - 90 EP - 7 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 124 N2 - Two novel polysaccharides termed PLPS-1 and PLPS-2 were isolated from mycelia of cultured Phellinus linteus by hot water extraction, purified by DEAE-52 cellulose and Sephadex G-100 column chromatography, and structurally characterized by FTIR and NMR spectroscopy, GC-MS, periodate oxidation/Smith degradation, and methylation analysis. The monosaccharide compositions of PLPS-1 (MW 2.5×10(5)Da) and PLPS-2 (MW 2.8×10(4)Da) were respectively Glc, Ara, Fuc, Gal, and Xyl in molar ratio 21.964:1.336:1.182:1:1, and Glc, Gal, Man, Ara, Fuc, Xyl in molar ratio 14.368:2.594:1.956:1.552:1.466:1; i.e., both were heteropolysaccharides. The backbone of PLPS-1 consisted primarily of repeating α-d-Glc(1→4)-α-d-Glc(1→6) units, while that of PLPS-2 consisted of α-(1→3)-d-Glc and α-(1→6)-d-Glc. The side branches were also different in their carbohydrate components. In in vitro antitumor assays, PLPS-1 displayed strong anti-proliferative effect against S-180 sarcoma cells through apoptosis, whereas PLPS-2 had no such effect. The difference in antitumor activity between the two PLPS evidently results from their structural differences. PLPS-1 has potential as a novel anticancer agent. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/25839798/A_novel_polysaccharide_from_mycelia_of_cultured_Phellinus_linteus_displays_antitumor_activity_through_apoptosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0144-8617(15)00122-8 DB - PRIME DP - Unbound Medicine ER -