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Identification and expression analysis of two interleukin-23α (p19) isoforms, in rainbow trout Oncorhynchus mykiss and Atlantic salmon Salmo salar.
Mol Immunol 2015; 66(2):216-28MI

Abstract

Interleukin (IL)-23 is a heterodimeric IL-12 family cytokine composed of a p19 α-chain, linked to a p40 β-chain that is shared with IL-12. IL-23 is distinguished functionally from IL-12 by its ability to induce the production of IL-17, and differentiation of Th17 cells in mammals. Three isoforms of p40 (p40a, p40b and p40c) have been found in some 3R teleosts. Salmonids also possess three p40 isoforms (p40b1, p40b2 and p40c) although p40a is missing, and two copies (paralogues) of p40b are present that have presumably been retained following the 4R duplication in this fish lineage. Teleost p19 has been discovered recently in zebrafish, but to date there is limited information on expression and modulation of this molecule. In this report we have cloned two p19 paralogues (p19a and p19b) in salmonids, suggesting that a salmonid can possess six potential IL-23 isoforms. Whilst Atlantic salmon has two active p19 genes, the rainbow trout p19b gene may have been pseudogenized. The salmonid p19 translations share moderate identities (22.8-29.9%) to zebrafish and mammalian p19 molecules, but their identity was supported by structural features, a conserved 4 exon/3 intron gene organisation, and phylogenetic tree analysis. The active salmonid p19 genes are highly expressed in blood and gonad. Bacterial (Yersinia ruckeri) and viral infection in rainbow trout induces the expression of p19a, suggesting pathogen-specific induction of IL-23 isoforms. Trout p19a expression was also induced by PAMPs (poly IC and peptidoglycan) and the proinflammatory cytokine IL-1β in primary head kidney macrophages. These data may indicate diverse functional roles of trout IL-23 isoforms in regulating the immune response in fish.

Authors+Show Affiliations

Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK; College of Fishery and Life Science, Shanghai Ocean University, Shanghai 201306, China.Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK; Key Laboratory of Aquaculture and Ecology of Coastal pool in Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China.Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK; Molecular Genetics, School of Science, University of Waikato, Hamilton, New Zealand.Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK. Electronic address: t.h.wang@abdn.ac.uk.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25841173

Citation

Jiang, Yousheng, et al. "Identification and Expression Analysis of Two Interleukin-23α (p19) Isoforms, in Rainbow Trout Oncorhynchus Mykiss and Atlantic Salmon Salmo Salar." Molecular Immunology, vol. 66, no. 2, 2015, pp. 216-28.
Jiang Y, Husain M, Qi Z, et al. Identification and expression analysis of two interleukin-23α (p19) isoforms, in rainbow trout Oncorhynchus mykiss and Atlantic salmon Salmo salar. Mol Immunol. 2015;66(2):216-28.
Jiang, Y., Husain, M., Qi, Z., Bird, S., & Wang, T. (2015). Identification and expression analysis of two interleukin-23α (p19) isoforms, in rainbow trout Oncorhynchus mykiss and Atlantic salmon Salmo salar. Molecular Immunology, 66(2), pp. 216-28. doi:10.1016/j.molimm.2015.03.014.
Jiang Y, et al. Identification and Expression Analysis of Two Interleukin-23α (p19) Isoforms, in Rainbow Trout Oncorhynchus Mykiss and Atlantic Salmon Salmo Salar. Mol Immunol. 2015;66(2):216-28. PubMed PMID: 25841173.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and expression analysis of two interleukin-23α (p19) isoforms, in rainbow trout Oncorhynchus mykiss and Atlantic salmon Salmo salar. AU - Jiang,Yousheng, AU - Husain,Mansourah, AU - Qi,Zhitao, AU - Bird,Steve, AU - Wang,Tiehui, Y1 - 2015/04/01/ PY - 2015/02/15/received PY - 2015/03/11/revised PY - 2015/03/12/accepted PY - 2015/4/5/entrez PY - 2015/4/5/pubmed PY - 2015/8/12/medline KW - Atlantic salmon Salmo salar KW - Cloning KW - Expression KW - IL-23 KW - Rainbow trout Oncorhynchus mykiss KW - p19 SP - 216 EP - 28 JF - Molecular immunology JO - Mol. Immunol. VL - 66 IS - 2 N2 - Interleukin (IL)-23 is a heterodimeric IL-12 family cytokine composed of a p19 α-chain, linked to a p40 β-chain that is shared with IL-12. IL-23 is distinguished functionally from IL-12 by its ability to induce the production of IL-17, and differentiation of Th17 cells in mammals. Three isoforms of p40 (p40a, p40b and p40c) have been found in some 3R teleosts. Salmonids also possess three p40 isoforms (p40b1, p40b2 and p40c) although p40a is missing, and two copies (paralogues) of p40b are present that have presumably been retained following the 4R duplication in this fish lineage. Teleost p19 has been discovered recently in zebrafish, but to date there is limited information on expression and modulation of this molecule. In this report we have cloned two p19 paralogues (p19a and p19b) in salmonids, suggesting that a salmonid can possess six potential IL-23 isoforms. Whilst Atlantic salmon has two active p19 genes, the rainbow trout p19b gene may have been pseudogenized. The salmonid p19 translations share moderate identities (22.8-29.9%) to zebrafish and mammalian p19 molecules, but their identity was supported by structural features, a conserved 4 exon/3 intron gene organisation, and phylogenetic tree analysis. The active salmonid p19 genes are highly expressed in blood and gonad. Bacterial (Yersinia ruckeri) and viral infection in rainbow trout induces the expression of p19a, suggesting pathogen-specific induction of IL-23 isoforms. Trout p19a expression was also induced by PAMPs (poly IC and peptidoglycan) and the proinflammatory cytokine IL-1β in primary head kidney macrophages. These data may indicate diverse functional roles of trout IL-23 isoforms in regulating the immune response in fish. SN - 1872-9142 UR - https://www.unboundmedicine.com/medline/citation/25841173/Identification_and_expression_analysis_of_two_interleukin_23α__p19__isoforms_in_rainbow_trout_Oncorhynchus_mykiss_and_Atlantic_salmon_Salmo_salar_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0161-5890(15)00118-2 DB - PRIME DP - Unbound Medicine ER -