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Acute effect of 3β-hidroxihop-22(29)ene on insulin secretion is mediated by GLP-1, potassium and calcium channels for the glucose homeostasis.
J Steroid Biochem Mol Biol 2015; 150:112-22JS

Abstract

The effect of 3β-hidroxihop-22(29)ene (3-BHO) on insulin and glucagon-like peptide 1 (GLP-1) secretion as well as the mechanism of action of the compound in pancreatic islet on glucose homeostasis was investigated. The data from in vivo treatment show that 3-BHO significantly reduces the hyperglycemia by increasing the insulin and GLP-1 secretion, as well as by accumulating hepatic glycogen in hyperglycemic rats. In rat pancreatic β-cell, 3-BHO stimulates the glucose uptake, insulin vesicles translocation to the plasma membrane and thus the insulin secretion through the involvement of potassium channels (ATP- and Ca(2+)-dependent K(+) channels) and calcium channels (L-type voltage-dependent calcium channels (L-VDCC)). Furthermore, this study also provides evidence for a crosstalk between intracellular high calcium concentration, PKA and PKC in the signal transduction of 3-BHO to stimulate insulin secretion. In conclusion, 3-BHO diminishes glycaemia, stimulates GLP-1 secretion and potentiates insulin secretion and increase hepatic glycogen content. Moreover, this triterpene modulates calcium influx characterizing ATP-K(+), Ca(2+)-K(+) and L-VDCC channels-dependent pathways as well as PKA and PKC activity in pancreatic islets underlying the signaling of 3-BHO for the secretory activity and contribution on glucose homeostasis.

Authors+Show Affiliations

Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx. Postal 5069, CEP 88040-900, Florianópolis, SC, Brazil.Universidade Federal da Fronteira Sul, Campus Universitário Laranjeiras do Sul, Laranjeiras do Sul, PR, Brazil.Departamento de Química, Centro de Ciências Físicas e Matemáticas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Florianópolis, SC, Brazil.Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx. Postal 5069, CEP 88040-900, Florianópolis, SC, Brazil.Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx. Postal 5069, CEP 88040-900, Florianópolis, SC, Brazil.Universidade Federal de Santa Catarina, Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Campus Universitário, Bairro Trindade, Florianópolis, SC, Brazil.Departamento de Química, Centro de Ciências Físicas e Matemáticas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Florianópolis, SC, Brazil.Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx. Postal 5069, CEP 88040-900, Florianópolis, SC, Brazil. Electronic address: mena.barreto@ufsc.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25843210

Citation

Castro, Allisson Jhonatan Gomes, et al. "Acute Effect of 3β-hidroxihop-22(29)ene On Insulin Secretion Is Mediated By GLP-1, Potassium and Calcium Channels for the Glucose Homeostasis." The Journal of Steroid Biochemistry and Molecular Biology, vol. 150, 2015, pp. 112-22.
Castro AJ, Cazarolli LH, de Carvalho FK, et al. Acute effect of 3β-hidroxihop-22(29)ene on insulin secretion is mediated by GLP-1, potassium and calcium channels for the glucose homeostasis. J Steroid Biochem Mol Biol. 2015;150:112-22.
Castro, A. J., Cazarolli, L. H., de Carvalho, F. K., da Luz, G., Altenhofen, D., dos Santos, A. R., ... Silva, F. R. (2015). Acute effect of 3β-hidroxihop-22(29)ene on insulin secretion is mediated by GLP-1, potassium and calcium channels for the glucose homeostasis. The Journal of Steroid Biochemistry and Molecular Biology, 150, pp. 112-22. doi:10.1016/j.jsbmb.2015.03.011.
Castro AJ, et al. Acute Effect of 3β-hidroxihop-22(29)ene On Insulin Secretion Is Mediated By GLP-1, Potassium and Calcium Channels for the Glucose Homeostasis. J Steroid Biochem Mol Biol. 2015;150:112-22. PubMed PMID: 25843210.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acute effect of 3β-hidroxihop-22(29)ene on insulin secretion is mediated by GLP-1, potassium and calcium channels for the glucose homeostasis. AU - Castro,Allisson Jhonatan Gomes, AU - Cazarolli,Luisa Helena, AU - de Carvalho,Francieli Kanumfre, AU - da Luz,Gabrielle, AU - Altenhofen,Delsi, AU - dos Santos,Adair Roberto Soares, AU - Pizzolatti,Moacir Geraldo, AU - Silva,Fátima Regina Mena Barreto, Y1 - 2015/04/02/ PY - 2014/11/25/received PY - 2015/03/05/revised PY - 2015/03/17/accepted PY - 2015/4/7/entrez PY - 2015/4/7/pubmed PY - 2015/7/21/medline KW - Calcium KW - Glycaemia KW - Insulin KW - Pancreas KW - Potassium KW - Triterpenes SP - 112 EP - 22 JF - The Journal of steroid biochemistry and molecular biology JO - J. Steroid Biochem. Mol. Biol. VL - 150 N2 - The effect of 3β-hidroxihop-22(29)ene (3-BHO) on insulin and glucagon-like peptide 1 (GLP-1) secretion as well as the mechanism of action of the compound in pancreatic islet on glucose homeostasis was investigated. The data from in vivo treatment show that 3-BHO significantly reduces the hyperglycemia by increasing the insulin and GLP-1 secretion, as well as by accumulating hepatic glycogen in hyperglycemic rats. In rat pancreatic β-cell, 3-BHO stimulates the glucose uptake, insulin vesicles translocation to the plasma membrane and thus the insulin secretion through the involvement of potassium channels (ATP- and Ca(2+)-dependent K(+) channels) and calcium channels (L-type voltage-dependent calcium channels (L-VDCC)). Furthermore, this study also provides evidence for a crosstalk between intracellular high calcium concentration, PKA and PKC in the signal transduction of 3-BHO to stimulate insulin secretion. In conclusion, 3-BHO diminishes glycaemia, stimulates GLP-1 secretion and potentiates insulin secretion and increase hepatic glycogen content. Moreover, this triterpene modulates calcium influx characterizing ATP-K(+), Ca(2+)-K(+) and L-VDCC channels-dependent pathways as well as PKA and PKC activity in pancreatic islets underlying the signaling of 3-BHO for the secretory activity and contribution on glucose homeostasis. SN - 1879-1220 UR - https://www.unboundmedicine.com/medline/citation/25843210/Acute_effect_of_3β_hidroxihop_22_29_ene_on_insulin_secretion_is_mediated_by_GLP_1_potassium_and_calcium_channels_for_the_glucose_homeostasis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-0760(15)00096-5 DB - PRIME DP - Unbound Medicine ER -