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Carboxylate groups play a major role in antitumor activity of Ganoderma applanatum polysaccharide.
Carbohydr Polym. 2015 Jun 05; 123:283-7.CP

Abstract

In this paper, the structure difference between the polysaccharides isolated from fruit bodies (FGAP) and submerged fermentation system (SGAP) of Ganoderma applanatum was investigated by means of GPC, HPLC and IR, respectively. And their antitumor activities were evaluated against Sarcoma 180 in vivo. The results showed that FGAP and SGAP were typical polysaccharides with different molecular weights, monosaccharide components, and functional groups. Closely related to the distinct structures, FGAP exhibited a better antitumor activity than SGAP. Moreover, since FGAP contained carboxylate groups rather than SGAP, such groups were chemically introduced into SGAP (CSGAP) by carboxymethylation in order to identify their contribution to antitumor activity. The results demonstrated that the inhibition of CSGAP against Sarcoma 180 in vivo was significantly enhanced by comparison to the native SGAP and even higher than that of FGAP, suggesting that the carboxylate groups played a major role in antitumor activity of G. applanatum polysaccharide.

Authors+Show Affiliations

College of Chemical and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, PR China.College of Chemical and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, PR China.College of Chemical and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, PR China.College of Chemical and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, PR China. Electronic address: jpwang@qau.edu.cn.Faculty of Education, University of Ottawa, Ottawa, ON K1N6N5, Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25843860

Citation

Sun, Xiaobo, et al. "Carboxylate Groups Play a Major Role in Antitumor Activity of Ganoderma Applanatum Polysaccharide." Carbohydrate Polymers, vol. 123, 2015, pp. 283-7.
Sun X, Zhao C, Pan W, et al. Carboxylate groups play a major role in antitumor activity of Ganoderma applanatum polysaccharide. Carbohydr Polym. 2015;123:283-7.
Sun, X., Zhao, C., Pan, W., Wang, J., & Wang, W. (2015). Carboxylate groups play a major role in antitumor activity of Ganoderma applanatum polysaccharide. Carbohydrate Polymers, 123, 283-7. https://doi.org/10.1016/j.carbpol.2015.01.062
Sun X, et al. Carboxylate Groups Play a Major Role in Antitumor Activity of Ganoderma Applanatum Polysaccharide. Carbohydr Polym. 2015 Jun 5;123:283-7. PubMed PMID: 25843860.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carboxylate groups play a major role in antitumor activity of Ganoderma applanatum polysaccharide. AU - Sun,Xiaobo, AU - Zhao,Chen, AU - Pan,Wei, AU - Wang,Jinping, AU - Wang,Weijun, Y1 - 2015/02/04/ PY - 2014/12/18/received PY - 2015/01/18/revised PY - 2015/01/26/accepted PY - 2015/4/7/entrez PY - 2015/4/7/pubmed PY - 2015/12/29/medline KW - Antitumor KW - Carboxylate KW - Carboxymethylation KW - Polysaccharide SP - 283 EP - 7 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 123 N2 - In this paper, the structure difference between the polysaccharides isolated from fruit bodies (FGAP) and submerged fermentation system (SGAP) of Ganoderma applanatum was investigated by means of GPC, HPLC and IR, respectively. And their antitumor activities were evaluated against Sarcoma 180 in vivo. The results showed that FGAP and SGAP were typical polysaccharides with different molecular weights, monosaccharide components, and functional groups. Closely related to the distinct structures, FGAP exhibited a better antitumor activity than SGAP. Moreover, since FGAP contained carboxylate groups rather than SGAP, such groups were chemically introduced into SGAP (CSGAP) by carboxymethylation in order to identify their contribution to antitumor activity. The results demonstrated that the inhibition of CSGAP against Sarcoma 180 in vivo was significantly enhanced by comparison to the native SGAP and even higher than that of FGAP, suggesting that the carboxylate groups played a major role in antitumor activity of G. applanatum polysaccharide. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/25843860/Carboxylate_groups_play_a_major_role_in_antitumor_activity_of_Ganoderma_applanatum_polysaccharide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0144-8617(15)00093-4 DB - PRIME DP - Unbound Medicine ER -