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Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu.
BMC Plant Biol. 2015 Feb 28; 15:68.BP

Abstract

BACKGROUND

Wheat (AABBDD, 2n = 6x = 42) is a major dietary component for many populations across the world. Bread-making quality of wheat is mainly determined by glutenin subunits, but it remains challenging to elucidate the composition and variation of low-molecular-weight glutenin subunits (LMW-GS) genes, the major components for glutenin subunits in hexaploid wheat. This problem, however, can be greatly simplified by characterizing the LMW-GS genes in Triticum urartu, the A-genome donor of hexaploid wheat. In the present study, we exploited the high-throughput molecular marker system, gene cloning, proteomic methods and molecular evolutionary genetic analysis to reveal the composition, variation, expression and evolution of LMW-GS genes in a T. urartu population from the Fertile Crescent region.

RESULTS

Eight LMW-GS genes, including four m-type, one s-type and three i-type, were characterized in the T. urartu population. Six or seven genes, the highest number at the Glu-A3 locus, were detected in each accession. Three i-type genes, each containing more than six allelic variants, were tightly linked because of their co-segregation in every accession. Only 2-3 allelic variants were detected for each m- and s-type gene. The m-type gene, TuA3-385, for which homologs were previously characterized only at Glu-D3 locus in common wheat and Aegilops tauschii, was detected at Glu-A3 locus in T. urartu. TuA3-460 was the first s-type gene identified at Glu-A3 locus. Proteomic analysis showed 1-4 genes, mainly i-type, expressed in individual accessions. About 62% accessions had three active i-type genes, rather than one or two in common wheat. Southeastern Turkey might be the center of origin and diversity for T. urartu due to its abundance of LMW-GS genes/genotypes. Phylogenetic reconstruction demonstrated that the characterized T. urartu might be the direct donor of the Glu-A3 locus in common wheat varieties.

CONCLUSIONS

Compared with the Glu-A3 locus in common wheat, a large number of highly diverse LMW-GS genes and active genes were characterized in T. urartu, demonstrating that this progenitor might provide valuable genetic resources for LMW-GS genes to improve the quality of common wheat. The phylogenetic analysis provided molecular evidence and confirmed that T. urartu was the A-genome donor of hexaploid wheat.

Authors

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Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25849991

Citation

Luo, Guangbin, et al. "Composition, Variation, Expression and Evolution of Low-molecular-weight Glutenin Subunit Genes in Triticum Urartu." BMC Plant Biology, vol. 15, 2015, p. 68.
Luo G, Zhang X, Zhang Y, et al. Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu. BMC Plant Biol. 2015;15:68.
Luo, G., Zhang, X., Zhang, Y., Yang, W., Li, Y., Sun, J., Zhan, K., Zhang, A., & Liu, D. (2015). Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu. BMC Plant Biology, 15, 68. https://doi.org/10.1186/s12870-014-0322-3
Luo G, et al. Composition, Variation, Expression and Evolution of Low-molecular-weight Glutenin Subunit Genes in Triticum Urartu. BMC Plant Biol. 2015 Feb 28;15:68. PubMed PMID: 25849991.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu. AU - Luo,Guangbin, AU - Zhang,Xiaofei, AU - Zhang,Yanlin, AU - Yang,Wenlong, AU - Li,Yiwen, AU - Sun,Jiazhu, AU - Zhan,Kehui, AU - Zhang,Aimin, AU - Liu,Dongcheng, Y1 - 2015/02/28/ PY - 2014/07/25/received PY - 2014/11/06/accepted PY - 2015/4/8/entrez PY - 2015/4/8/pubmed PY - 2016/1/8/medline SP - 68 EP - 68 JF - BMC plant biology JO - BMC Plant Biol VL - 15 N2 - BACKGROUND: Wheat (AABBDD, 2n = 6x = 42) is a major dietary component for many populations across the world. Bread-making quality of wheat is mainly determined by glutenin subunits, but it remains challenging to elucidate the composition and variation of low-molecular-weight glutenin subunits (LMW-GS) genes, the major components for glutenin subunits in hexaploid wheat. This problem, however, can be greatly simplified by characterizing the LMW-GS genes in Triticum urartu, the A-genome donor of hexaploid wheat. In the present study, we exploited the high-throughput molecular marker system, gene cloning, proteomic methods and molecular evolutionary genetic analysis to reveal the composition, variation, expression and evolution of LMW-GS genes in a T. urartu population from the Fertile Crescent region. RESULTS: Eight LMW-GS genes, including four m-type, one s-type and three i-type, were characterized in the T. urartu population. Six or seven genes, the highest number at the Glu-A3 locus, were detected in each accession. Three i-type genes, each containing more than six allelic variants, were tightly linked because of their co-segregation in every accession. Only 2-3 allelic variants were detected for each m- and s-type gene. The m-type gene, TuA3-385, for which homologs were previously characterized only at Glu-D3 locus in common wheat and Aegilops tauschii, was detected at Glu-A3 locus in T. urartu. TuA3-460 was the first s-type gene identified at Glu-A3 locus. Proteomic analysis showed 1-4 genes, mainly i-type, expressed in individual accessions. About 62% accessions had three active i-type genes, rather than one or two in common wheat. Southeastern Turkey might be the center of origin and diversity for T. urartu due to its abundance of LMW-GS genes/genotypes. Phylogenetic reconstruction demonstrated that the characterized T. urartu might be the direct donor of the Glu-A3 locus in common wheat varieties. CONCLUSIONS: Compared with the Glu-A3 locus in common wheat, a large number of highly diverse LMW-GS genes and active genes were characterized in T. urartu, demonstrating that this progenitor might provide valuable genetic resources for LMW-GS genes to improve the quality of common wheat. The phylogenetic analysis provided molecular evidence and confirmed that T. urartu was the A-genome donor of hexaploid wheat. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/25849991/Composition_variation_expression_and_evolution_of_low_molecular_weight_glutenin_subunit_genes_in_Triticum_urartu_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0322-3 DB - PRIME DP - Unbound Medicine ER -