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Determination of cyproheptadine in feeds using molecularly imprinted solid-phase extraction coupled with HPLC.
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 May 15; 990:39-44.JC

Abstract

A novel method was developed for the determination of cyproheptadine in feeds using molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography. The polymers were prepared using cyproheptadine as a template molecule, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linking agent, and dichloromethane as a solvent by bulk polymerization. Under the optimum solid-phase extraction conditions, the molecular imprinting cartridge can selectively extract and enrich cyproheptadine from a variety of feeds. Mean recoveries of cyproheptadine from four kinds of feeds spiked at 0.1, 1.0 and 10mgkg(-1) ranged from 85.5% to 96.2%, with intra-day and inter-day relative standard deviation less than 10%. The calibration curve of cyproheptadine was good linear relationship (r>0.9993) within the range of 0.1-50μgmL(-1). The limit of detection (LOD) and the limit of quantification (LOQ) were 0.04 and 0.1mgkg(-1), respectively.

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Publisher Full Text (DOI)

Authors+Show Affiliations

National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: liminokhe@scau.edu.cn.

MeSH

Animal FeedChromatography, High Pressure LiquidCyproheptadineLinear ModelsMolecular ImprintingReproducibility of ResultsSensitivity and SpecificitySolid Phase Extraction

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25855316

Citation

Yang, Jianwen, et al. "Determination of Cyproheptadine in Feeds Using Molecularly Imprinted Solid-phase Extraction Coupled With HPLC." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 990, 2015, pp. 39-44.

Yang J, Wang Z, Zhou T, et al. Determination of cyproheptadine in feeds using molecularly imprinted solid-phase extraction coupled with HPLC. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;990:39-44.

Yang, J., Wang, Z., Zhou, T., Song, X., Liu, Q., Zhang, Y., & He, L. (2015). Determination of cyproheptadine in feeds using molecularly imprinted solid-phase extraction coupled with HPLC. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 990, 39-44. https://doi.org/10.1016/j.jchromb.2015.03.009

Yang J, et al. Determination of Cyproheptadine in Feeds Using Molecularly Imprinted Solid-phase Extraction Coupled With HPLC. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 May 15;990:39-44. PubMed PMID: 25855316.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Determination of cyproheptadine in feeds using molecularly imprinted solid-phase extraction coupled with HPLC. AU - Yang,Jianwen, AU - Wang,Zongnan, AU - Zhou,Tong, AU - Song,Xuqin, AU - Liu,Qingyong, AU - Zhang,Yuman, AU - He,Limin, Y1 - 2015/03/24/ PY - 2014/12/13/received PY - 2015/03/16/revised PY - 2015/03/18/accepted PY - 2015/4/10/entrez PY - 2015/4/10/pubmed PY - 2016/1/26/medline KW - Cyproheptadine KW - Feeds KW - High performance liquid chromatography KW - Molecularly imprinted polymers SP - 39 EP - 44 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 990 N2 - A novel method was developed for the determination of cyproheptadine in feeds using molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography. The polymers were prepared using cyproheptadine as a template molecule, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linking agent, and dichloromethane as a solvent by bulk polymerization. Under the optimum solid-phase extraction conditions, the molecular imprinting cartridge can selectively extract and enrich cyproheptadine from a variety of feeds. Mean recoveries of cyproheptadine from four kinds of feeds spiked at 0.1, 1.0 and 10mgkg(-1) ranged from 85.5% to 96.2%, with intra-day and inter-day relative standard deviation less than 10%. The calibration curve of cyproheptadine was good linear relationship (r>0.9993) within the range of 0.1-50μgmL(-1). The limit of detection (LOD) and the limit of quantification (LOQ) were 0.04 and 0.1mgkg(-1), respectively. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/25855316/Determination_of_cyproheptadine_in_feeds_using_molecularly_imprinted_solid_phase_extraction_coupled_with_HPLC_ DB - PRIME DP - Unbound Medicine ER -