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Free radical scavenging actions of three Trifolium species in the protection of blood plasma antioxidant capacity in vitro.
Pharm Biol. 2015; 53(9):1277-84.PB

Abstract

CONTEXT

Three clover [Trifolium L. (Leguminosae)] species were selected on the basis of data from traditional medicine, phytochemical profiles, and agricultural significance.

OBJECTIVE

The in vitro evaluations of free radical scavenging properties, ferric reducing abilities, and antioxidant effects of extracts from T. pratense L. (crude extract and phenolic fraction), T. pallidum L., and T. scabrum L. (phenolic fractions) were performed.

MATERIALS AND METHODS

Activities of the Trifolium extracts were determined at their final concentrations of 1.5-50 µg/ml. Free radical scavenging properties of methanol extract solutions were estimated by the reduction of DPPH(•) and ABTS(•) radicals. Measurements of the total antioxidant capacity (TAC) were carried out to assess the antioxidant activities of the extracts in human blood plasma under conditions of oxidative stress, induced by 200 μM peroxynitrite.

RESULTS

The phenolic fraction of T. pratense displayed the strongest ABTS(•) and DPPH(•) radical scavenging effects (EC50 value of 21.69 and 12.27 µg/ml, respectively). The EC50 value for T. pallidum extract attained 29.77 and 30.06 µg/ml. The two remaining extracts were less potent scavengers (EC50 value higher than 50 µg/ml). Similar differences were obtained during evaluation of the ferric reducing abilities. Analysis of antioxidant properties of the extracts in blood plasma did not provide such evident differences in their actions, however, it indicated that the T. pratense phenolic fraction displayed the strongest effect.

CONCLUSIONS

The examined Trifolium extracts partly protected blood plasma and enhanced its non-enzymatic antioxidant defense against harmful action of peroxynitrite in vitro.

Authors+Show Affiliations

Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz , Lodz , Poland and.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25856697

Citation

Kolodziejczyk-Czepas, Joanna, et al. "Free Radical Scavenging Actions of Three Trifolium Species in the Protection of Blood Plasma Antioxidant Capacity in Vitro." Pharmaceutical Biology, vol. 53, no. 9, 2015, pp. 1277-84.
Kolodziejczyk-Czepas J, Nowak P, Moniuszko-Szajwaj B, et al. Free radical scavenging actions of three Trifolium species in the protection of blood plasma antioxidant capacity in vitro. Pharm Biol. 2015;53(9):1277-84.
Kolodziejczyk-Czepas, J., Nowak, P., Moniuszko-Szajwaj, B., Kowalska, I., & Stochmal, A. (2015). Free radical scavenging actions of three Trifolium species in the protection of blood plasma antioxidant capacity in vitro. Pharmaceutical Biology, 53(9), 1277-84. https://doi.org/10.3109/13880209.2014.974064
Kolodziejczyk-Czepas J, et al. Free Radical Scavenging Actions of Three Trifolium Species in the Protection of Blood Plasma Antioxidant Capacity in Vitro. Pharm Biol. 2015;53(9):1277-84. PubMed PMID: 25856697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Free radical scavenging actions of three Trifolium species in the protection of blood plasma antioxidant capacity in vitro. AU - Kolodziejczyk-Czepas,Joanna, AU - Nowak,Pawel, AU - Moniuszko-Szajwaj,Barbara, AU - Kowalska,Iwona, AU - Stochmal,Anna, Y1 - 2015/04/09/ PY - 2015/4/10/entrez PY - 2015/4/10/pubmed PY - 2016/4/12/medline KW - Clover KW - oxidative stress KW - peroxynitrite SP - 1277 EP - 84 JF - Pharmaceutical biology JO - Pharm Biol VL - 53 IS - 9 N2 - CONTEXT: Three clover [Trifolium L. (Leguminosae)] species were selected on the basis of data from traditional medicine, phytochemical profiles, and agricultural significance. OBJECTIVE: The in vitro evaluations of free radical scavenging properties, ferric reducing abilities, and antioxidant effects of extracts from T. pratense L. (crude extract and phenolic fraction), T. pallidum L., and T. scabrum L. (phenolic fractions) were performed. MATERIALS AND METHODS: Activities of the Trifolium extracts were determined at their final concentrations of 1.5-50 µg/ml. Free radical scavenging properties of methanol extract solutions were estimated by the reduction of DPPH(•) and ABTS(•) radicals. Measurements of the total antioxidant capacity (TAC) were carried out to assess the antioxidant activities of the extracts in human blood plasma under conditions of oxidative stress, induced by 200 μM peroxynitrite. RESULTS: The phenolic fraction of T. pratense displayed the strongest ABTS(•) and DPPH(•) radical scavenging effects (EC50 value of 21.69 and 12.27 µg/ml, respectively). The EC50 value for T. pallidum extract attained 29.77 and 30.06 µg/ml. The two remaining extracts were less potent scavengers (EC50 value higher than 50 µg/ml). Similar differences were obtained during evaluation of the ferric reducing abilities. Analysis of antioxidant properties of the extracts in blood plasma did not provide such evident differences in their actions, however, it indicated that the T. pratense phenolic fraction displayed the strongest effect. CONCLUSIONS: The examined Trifolium extracts partly protected blood plasma and enhanced its non-enzymatic antioxidant defense against harmful action of peroxynitrite in vitro. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25856697/Free_radical_scavenging_actions_of_three_Trifolium_species_in_the_protection_of_blood_plasma_antioxidant_capacity_in_vitro_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2014.974064 DB - PRIME DP - Unbound Medicine ER -