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Antioxidant and hepatoprotective activities of the oil fractions from wild carrot (Daucus carota ssp. carota).
Pharm Biol. 2015; 53(9):1285-94.PB

Abstract

CONTEXT

Wild carrot, Daucus carota L. ssp. carota (Apiacae), is widely distributed throughout the world and has various uses in traditional medicine in Lebanon.

OBJECTIVE

The present study aimed to fractionate and analyze the chemical composition of the Daucus carota oil extract (DCOE) fractions and to evaluate their antioxidant and hepatoprotective properties in vitro and in vivo.

MATERIALS AND METHODS

DCOE was chromatographed on silica gel column to produce four fractions: pentane (F1), 50:50 pentane:diethyl ether (F2), diethyl ether (F3), and 93:7 chloroform: methanol (F4). Qualitative and quantitative analyses of oil fractions were performed by GC-MS and HPLC techniques. The in vitro antioxidant properties were assessed using DPPH, FIC, and ferric-reducing antioxidant power (FRAP) assays. The hepatoprotective property was determined by examining the levels of serum markers (alanine transaminase (ALT) and aspartate transaminase (AST)) and hepatic antioxidant (superoxide dismutase (SOD), catalase (CAT), and glutathione-S-transferase (GST)) enzymes in CCl4-intoxicated mice pretreated with intraperitoenal 50, 100, or 200 mg/kg b.w. of the oil fractions for 5 d.

RESULTS

GCMS analysis of F2 revealed the presence of 2-himachalen-6-ol (61.4%) which is reported for the first time in Daucus carota species. F3 and F4 were rich in phenolics and flavonoids and demonstrated significant DPPH activity (IC50 = 0.29 and 0.38 mg/ml, respectively) and high FRAP values (225.11 and 437.59 µmol FeSO4/g, respectively). The sesquiterpene-rich fraction F1 had the highest FIC ability (IC50 = 0.28 mg/ml). Pretreatment with F1 and F4 reversed the CCl4-induced decrease in SOD, CAT, and GST levels and reduced significantly hepatic damage.

DISCUSSION AND CONCLUSION

The current results suggested that wild carrot oil fractions exhibited a unique chemical composition and possessed significant antioxidant activities as well as hepatoprotective effects against CCl4-induced hepatotoxicity.

Authors+Show Affiliations

Department of Microbial and Cellular Sciences, Faculty of Health and Medical Sciences, University of Surrey , UK .No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25856705

Citation

Shebaby, Wassim N., et al. "Antioxidant and Hepatoprotective Activities of the Oil Fractions From Wild Carrot (Daucus Carota Ssp. Carota)." Pharmaceutical Biology, vol. 53, no. 9, 2015, pp. 1285-94.
Shebaby WN, Daher CF, El-Sibai M, et al. Antioxidant and hepatoprotective activities of the oil fractions from wild carrot (Daucus carota ssp. carota). Pharm Biol. 2015;53(9):1285-94.
Shebaby, W. N., Daher, C. F., El-Sibai, M., Bodman-Smith, K., Mansour, A., Karam, M. C., & Mroueh, M. (2015). Antioxidant and hepatoprotective activities of the oil fractions from wild carrot (Daucus carota ssp. carota). Pharmaceutical Biology, 53(9), 1285-94. https://doi.org/10.3109/13880209.2014.976349
Shebaby WN, et al. Antioxidant and Hepatoprotective Activities of the Oil Fractions From Wild Carrot (Daucus Carota Ssp. Carota). Pharm Biol. 2015;53(9):1285-94. PubMed PMID: 25856705.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and hepatoprotective activities of the oil fractions from wild carrot (Daucus carota ssp. carota). AU - Shebaby,Wassim N, AU - Daher,Costantine F, AU - El-Sibai,Mirvat, AU - Bodman-Smith,Kikki, AU - Mansour,Anthony, AU - Karam,Marc C, AU - Mroueh,Mohamad, Y1 - 2015/04/09/ PY - 2015/4/10/entrez PY - 2015/4/10/pubmed PY - 2016/4/12/medline KW - CAT KW - DPPH KW - FIC KW - FRAP KW - GST KW - SOD KW - flavonoids KW - phenolics SP - 1285 EP - 94 JF - Pharmaceutical biology JO - Pharm Biol VL - 53 IS - 9 N2 - CONTEXT: Wild carrot, Daucus carota L. ssp. carota (Apiacae), is widely distributed throughout the world and has various uses in traditional medicine in Lebanon. OBJECTIVE: The present study aimed to fractionate and analyze the chemical composition of the Daucus carota oil extract (DCOE) fractions and to evaluate their antioxidant and hepatoprotective properties in vitro and in vivo. MATERIALS AND METHODS: DCOE was chromatographed on silica gel column to produce four fractions: pentane (F1), 50:50 pentane:diethyl ether (F2), diethyl ether (F3), and 93:7 chloroform: methanol (F4). Qualitative and quantitative analyses of oil fractions were performed by GC-MS and HPLC techniques. The in vitro antioxidant properties were assessed using DPPH, FIC, and ferric-reducing antioxidant power (FRAP) assays. The hepatoprotective property was determined by examining the levels of serum markers (alanine transaminase (ALT) and aspartate transaminase (AST)) and hepatic antioxidant (superoxide dismutase (SOD), catalase (CAT), and glutathione-S-transferase (GST)) enzymes in CCl4-intoxicated mice pretreated with intraperitoenal 50, 100, or 200 mg/kg b.w. of the oil fractions for 5 d. RESULTS: GCMS analysis of F2 revealed the presence of 2-himachalen-6-ol (61.4%) which is reported for the first time in Daucus carota species. F3 and F4 were rich in phenolics and flavonoids and demonstrated significant DPPH activity (IC50 = 0.29 and 0.38 mg/ml, respectively) and high FRAP values (225.11 and 437.59 µmol FeSO4/g, respectively). The sesquiterpene-rich fraction F1 had the highest FIC ability (IC50 = 0.28 mg/ml). Pretreatment with F1 and F4 reversed the CCl4-induced decrease in SOD, CAT, and GST levels and reduced significantly hepatic damage. DISCUSSION AND CONCLUSION: The current results suggested that wild carrot oil fractions exhibited a unique chemical composition and possessed significant antioxidant activities as well as hepatoprotective effects against CCl4-induced hepatotoxicity. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25856705/Antioxidant_and_hepatoprotective_activities_of_the_oil_fractions_from_wild_carrot__Daucus_carota_ssp__carota__ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2014.976349 DB - PRIME DP - Unbound Medicine ER -