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Chemical composition and pharmacological properties of the essential oils obtained seasonally from Lippia thymoides.
Pharm Biol. 2016; 54(1):25-34.PB

Abstract

CONTEXT

Lippia thymoides Mart. & Schauer (Verbenaceae) is used in folk medicine to treat wounds, fever, bronchitis, rheumatism, headaches, and weakness.

OBJECTIVE

This study determinates the chemical composition of essential oils from L. thymoides, obtained at during each of the four seasons and correlates with pharmacological properties.

MATERIALS AND METHODS

Essential oils were obtained by hydrodistillation and analyzed by gas chromatography coupled to mass spectroscopy (GC-MS). Antioxidant activity was determined by DPPH free radical scavenging and β-carotene bleaching methods. The antimicrobial assays were performed by minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) methods. Isolated rat aorta and uterus, and guinea-pig trachea were utilized to evaluate relaxant potential in pre-contracted smooth muscle.

RESULTS AND DISCUSSION

Essential oils from leaves of L. thymoides had the sesquiterpene β-caryophyllene (17.22-26.27%) as the major constituent followed by borneol (4.45-7.36%), camphor (3.22-8.61%), camphene (2.64-5.66%), and germacrene D (4.72-6.18%). In vitro assays showed that these essential oils do not have antioxidant activity, have antimicrobial selectivity to Gram-positive bacteria Staphylococcus aureus (MIC = 0.004 mg/mL and MMC = 0.26-10.19 mg/mL) and Micrococcus luteus (MIC = 0.03 mg/mL and MMC = 8.43 mg/mL), relax isolated rat aorta (EC50 = 305-544 μg/mL, with endothelium; and EC50 = 150-283 μg/mL, without endothelium), and uterus (EC50 = 74-257 μg/mL), and minor potency, isolated guinea-pig trachea.

CONCLUSIONS

Lippia thymoides is a source of natural products of pharmaceutical interest, being necessary additional studies to determine the substances involved in the biological activities.

Authors+Show Affiliations

a Núcleo de Estudos e Pesquisas em Plantas Medicinais, Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco , Petrolina , Pernambuco , Brazil . b Programa de Pós-Graduação em Biotecnologia, Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana , Feira de Santana , Bahia , Brazil .a Núcleo de Estudos e Pesquisas em Plantas Medicinais, Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco , Petrolina , Pernambuco , Brazil .a Núcleo de Estudos e Pesquisas em Plantas Medicinais, Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco , Petrolina , Pernambuco , Brazil .c Laboratório de Química de Produtos Naturais e Sintéticos Bioativos, Departamento de Ciências Exatas , Universidade Estadual de Feira de Santana, Feira de Santana , Bahia , Brazil .a Núcleo de Estudos e Pesquisas em Plantas Medicinais, Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco , Petrolina , Pernambuco , Brazil .a Núcleo de Estudos e Pesquisas em Plantas Medicinais, Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco , Petrolina , Pernambuco , Brazil . d Programa de Pós-Graduação em Recursos Naturais do Semi-Árido, Universidade Federal do Vale do São Francisco, Petrolina , Pernambuco , Brazil .a Núcleo de Estudos e Pesquisas em Plantas Medicinais, Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco , Petrolina , Pernambuco , Brazil . d Programa de Pós-Graduação em Recursos Naturais do Semi-Árido, Universidade Federal do Vale do São Francisco, Petrolina , Pernambuco , Brazil .e Laboratório de Microbiologia da Agroindústria , Universidade Estadual de Santa Cruz , Ilhéus, Bahia , Brazil , and.f Departamento de Ciências Biológicas , Universidade Estadual de Feira de Santana , Feira de Santana , Bahia , Brazil.c Laboratório de Química de Produtos Naturais e Sintéticos Bioativos, Departamento de Ciências Exatas , Universidade Estadual de Feira de Santana, Feira de Santana , Bahia , Brazil .b Programa de Pós-Graduação em Biotecnologia, Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana , Feira de Santana , Bahia , Brazil . c Laboratório de Química de Produtos Naturais e Sintéticos Bioativos, Departamento de Ciências Exatas , Universidade Estadual de Feira de Santana, Feira de Santana , Bahia , Brazil .

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25856708

Citation

Silva, Fabrício Souza, et al. "Chemical Composition and Pharmacological Properties of the Essential Oils Obtained Seasonally From Lippia Thymoides." Pharmaceutical Biology, vol. 54, no. 1, 2016, pp. 25-34.
Silva FS, Menezes PM, de Sá PG, et al. Chemical composition and pharmacological properties of the essential oils obtained seasonally from Lippia thymoides. Pharm Biol. 2016;54(1):25-34.
Silva, F. S., Menezes, P. M., de Sá, P. G., Oliveira, A. L., Souza, E. A., Almeida, J. R., de Lima, J. T., Uetanabaro, A. P., Silva, T. R., Peralta, E. D., & Lucchese, A. M. (2016). Chemical composition and pharmacological properties of the essential oils obtained seasonally from Lippia thymoides. Pharmaceutical Biology, 54(1), 25-34. https://doi.org/10.3109/13880209.2015.1005751
Silva FS, et al. Chemical Composition and Pharmacological Properties of the Essential Oils Obtained Seasonally From Lippia Thymoides. Pharm Biol. 2016;54(1):25-34. PubMed PMID: 25856708.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical composition and pharmacological properties of the essential oils obtained seasonally from Lippia thymoides. AU - Silva,Fabrício Souza, AU - Menezes,Pedro Modesto Nascimento, AU - de Sá,Pedro Guilherme Souza, AU - Oliveira,André Luís de Santana, AU - Souza,Eric Alencar Araújo, AU - Almeida,Jackson Roberto Guedes da Silva, AU - de Lima,Julianeli Tolentino, AU - Uetanabaro,Ana Paula Trovatti, AU - Silva,Tânia Regina dos Santos, AU - Peralta,Edna Dória, AU - Lucchese,Angélica Maria, Y1 - 2015/04/09/ PY - 2015/4/10/entrez PY - 2015/4/10/pubmed PY - 2016/9/22/medline KW - Antimicrobial activity KW - essential oils KW - sesquiterpenes KW - spasmolytic activity KW - tocolytic activity KW - vasorelaxation SP - 25 EP - 34 JF - Pharmaceutical biology JO - Pharm Biol VL - 54 IS - 1 N2 - CONTEXT: Lippia thymoides Mart. & Schauer (Verbenaceae) is used in folk medicine to treat wounds, fever, bronchitis, rheumatism, headaches, and weakness. OBJECTIVE: This study determinates the chemical composition of essential oils from L. thymoides, obtained at during each of the four seasons and correlates with pharmacological properties. MATERIALS AND METHODS: Essential oils were obtained by hydrodistillation and analyzed by gas chromatography coupled to mass spectroscopy (GC-MS). Antioxidant activity was determined by DPPH free radical scavenging and β-carotene bleaching methods. The antimicrobial assays were performed by minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) methods. Isolated rat aorta and uterus, and guinea-pig trachea were utilized to evaluate relaxant potential in pre-contracted smooth muscle. RESULTS AND DISCUSSION: Essential oils from leaves of L. thymoides had the sesquiterpene β-caryophyllene (17.22-26.27%) as the major constituent followed by borneol (4.45-7.36%), camphor (3.22-8.61%), camphene (2.64-5.66%), and germacrene D (4.72-6.18%). In vitro assays showed that these essential oils do not have antioxidant activity, have antimicrobial selectivity to Gram-positive bacteria Staphylococcus aureus (MIC = 0.004 mg/mL and MMC = 0.26-10.19 mg/mL) and Micrococcus luteus (MIC = 0.03 mg/mL and MMC = 8.43 mg/mL), relax isolated rat aorta (EC50 = 305-544 μg/mL, with endothelium; and EC50 = 150-283 μg/mL, without endothelium), and uterus (EC50 = 74-257 μg/mL), and minor potency, isolated guinea-pig trachea. CONCLUSIONS: Lippia thymoides is a source of natural products of pharmaceutical interest, being necessary additional studies to determine the substances involved in the biological activities. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25856708/Chemical_composition_and_pharmacological_properties_of_the_essential_oils_obtained_seasonally_from_Lippia_thymoides_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2015.1005751 DB - PRIME DP - Unbound Medicine ER -