Tags

Type your tag names separated by a space and hit enter

In vitro antioxidant and anti-cholinesterase activities of Rhizophora mucronata.
Pharm Biol. 2016; 54(1):118-29.PB

Abstract

CONTEXT

Rhizophora mucronata Lam. (Rhizophoraceae), commonly known as Asiatic mangrove, has been used traditionally among Asian countries as folk medicine.

OBJECTIVE

This study investigates the cholinesterase inhibitory potential and antioxidant activities of R. mucronata.

MATERIALS AND METHOD

Rhizophora mucronata leaves were successively extracted using solvents of varying polarity and a dosage of 100-500 µg/ml were used for each assay. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities were assessed according to the method of Ellman. In vitro antioxidant activity was assessed using free radical scavenging, reducing power, and metal-chelating activity (duration - 3 months). Total phenolic and flavonoid content were quantified spectrophotometrically. Compound characterization was done using column chromatography, NMR, FTIR, and LC-MS analysis.

RESULTS

Methanolic leaf extract (500 µg/ml) exhibited the highest inhibitory activity against AChE (92.73 ± 0.54%) and BuChE (98.98 ± 0.17%), with an IC50 value of 59.31 ± 0.35 and 51.72 ± 0.33 µg/ml, respectively. Among the different solvent extracts, methanolic extract exhibited the highest antioxidant activity with an IC50 value of 47.39 ± 0.43, 401.45 ± 18.52, 80.23 ± 0.70, and 316.47 ± 3.56 µg/ml for DPPH, hydroxyl, nitric oxide radical, and hydrogen peroxide, respectively. Total polyphenolic and flavonoid contents in methanolic extract were observed to be 598.13 ± 1.85 µg of gallic acid equivalent and 48.85 ± 0.70 μg of rutin equivalent/mg of extract. Compound characterization illustrated (+)-catechin as the bioactive compound responsible for cholinesterase inhibitory and antioxidant activities.

CONCLUSION

The presence of rich source of flavonoids, in particular catechin, might be responsible for its cholinesterase inhibitory and antioxidant activities.

Authors+Show Affiliations

a Department of Biotechnology , Alagappa University , Karaikudi , Tamil Nadu , India.a Department of Biotechnology , Alagappa University , Karaikudi , Tamil Nadu , India.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25856713

Citation

Suganthy, N, and K Pandima Devi. "In Vitro Antioxidant and Anti-cholinesterase Activities of Rhizophora Mucronata." Pharmaceutical Biology, vol. 54, no. 1, 2016, pp. 118-29.
Suganthy N, Devi KP. In vitro antioxidant and anti-cholinesterase activities of Rhizophora mucronata. Pharm Biol. 2016;54(1):118-29.
Suganthy, N., & Devi, K. P. (2016). In vitro antioxidant and anti-cholinesterase activities of Rhizophora mucronata. Pharmaceutical Biology, 54(1), 118-29. https://doi.org/10.3109/13880209.2015.1017886
Suganthy N, Devi KP. In Vitro Antioxidant and Anti-cholinesterase Activities of Rhizophora Mucronata. Pharm Biol. 2016;54(1):118-29. PubMed PMID: 25856713.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antioxidant and anti-cholinesterase activities of Rhizophora mucronata. AU - Suganthy,N, AU - Devi,K Pandima, Y1 - 2015/04/09/ PY - 2015/4/10/entrez PY - 2015/4/10/pubmed PY - 2016/9/22/medline KW - Alzheimer’s disease KW - butyryl cholinesterase KW - catechin KW - enzyme kinetics KW - total polyphenolic conent SP - 118 EP - 29 JF - Pharmaceutical biology JO - Pharm Biol VL - 54 IS - 1 N2 - CONTEXT: Rhizophora mucronata Lam. (Rhizophoraceae), commonly known as Asiatic mangrove, has been used traditionally among Asian countries as folk medicine. OBJECTIVE: This study investigates the cholinesterase inhibitory potential and antioxidant activities of R. mucronata. MATERIALS AND METHOD: Rhizophora mucronata leaves were successively extracted using solvents of varying polarity and a dosage of 100-500 µg/ml were used for each assay. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities were assessed according to the method of Ellman. In vitro antioxidant activity was assessed using free radical scavenging, reducing power, and metal-chelating activity (duration - 3 months). Total phenolic and flavonoid content were quantified spectrophotometrically. Compound characterization was done using column chromatography, NMR, FTIR, and LC-MS analysis. RESULTS: Methanolic leaf extract (500 µg/ml) exhibited the highest inhibitory activity against AChE (92.73 ± 0.54%) and BuChE (98.98 ± 0.17%), with an IC50 value of 59.31 ± 0.35 and 51.72 ± 0.33 µg/ml, respectively. Among the different solvent extracts, methanolic extract exhibited the highest antioxidant activity with an IC50 value of 47.39 ± 0.43, 401.45 ± 18.52, 80.23 ± 0.70, and 316.47 ± 3.56 µg/ml for DPPH, hydroxyl, nitric oxide radical, and hydrogen peroxide, respectively. Total polyphenolic and flavonoid contents in methanolic extract were observed to be 598.13 ± 1.85 µg of gallic acid equivalent and 48.85 ± 0.70 μg of rutin equivalent/mg of extract. Compound characterization illustrated (+)-catechin as the bioactive compound responsible for cholinesterase inhibitory and antioxidant activities. CONCLUSION: The presence of rich source of flavonoids, in particular catechin, might be responsible for its cholinesterase inhibitory and antioxidant activities. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25856713/In_vitro_antioxidant_and_anti_cholinesterase_activities_of_Rhizophora_mucronata_ DB - PRIME DP - Unbound Medicine ER -