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Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.
Biol Res. 2015 Mar 25; 48:19.BR

Abstract

BACKGROUND

To evaluate the hepatoprotective potential and invitro cytotoxicity studies of whole plant methanol extract of Rumex vesicarius L. Methanol extract at a dose of 100 mg/kg bw and 200 mg/kg bw were assessed for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of SGOT (Serum glutamic oxaloacetic transaminase), SGPT (Serum glutamic pyruvic transaminase), ALP (Alkaline phosphatase), TP (Total protein), TB (Total bilirubin) and SOD (Superoxide dismutase), CAT (Catalase), MDA (Malondialdehyde). The cytotoxicity of the same extract on HepG2 cell lines were also assessed using MTT assay method at the concentration of 62.5, 125, 250, 500 μg/ml.

RESULTS

Pretreatment of animals with whole plant methanol extracts of Rumex vesicarius L. significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver. The biochemical parameters in serum also improved in treated groups compared to the control and standard (silymarin) groups. Histopathological investigation further corroborated these biochemical observations. The cytotoxicity results indicated that the plant extract which were inhibitory to the proliferation of HepG2 cell line with IC50 value of 563.33 ± 0.8 μg/ml were not cytotoxic and appears to be safe.

CONCLUSIONS

Rumex vesicarius L. whole plant methanol extract exhibit hepatoprotective activity. However the cytotoxicity in HepG2 is inexplicable and warrants further study.

Links

PMC Free PDF

Authors

Tukappa N K A
No affiliation info available
Londonkar RL
No affiliation info available
Nayaka HB
No affiliation info available
Kumar C B S
No affiliation info available

MeSH

Alanine TransaminaseAlkaline PhosphataseAnimalsAnticarcinogenic AgentsAntioxidantsAspartate AminotransferasesBilirubinCarbon TetrachlorideCatalaseCell ProliferationChemical and Drug Induced Liver InjuryCytotoxinsDrinkingEatingFormazansHep G2 CellsHumansLiverMaleMalondialdehydeMethanolPhytotherapyPlant ExtractsRats, WistarRumexSilymarinSuperoxide DismutaseTetrazolium Salts

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25857314

Clinical Trial Links

Effect of Oral Administration of a Herbarium Mixture (Guazuma Ulmifolia and Tecoma Stans) on Metabolic Profile in Type 2 Diabetic Patients

Citation

Tukappa N K, Asha, et al. "Cytotoxicity and Hepatoprotective Attributes of Methanolic Extract of Rumex Vesicarius L." Biological Research, vol. 48, 2015, p. 19.
Tukappa N K A, Londonkar RL, Nayaka HB, et al. Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L. Biol Res. 2015;48:19.
Tukappa N K, A., Londonkar, R. L., Nayaka, H. B., & Kumar C B, S. (2015). Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L. Biological Research, 48, 19. https://doi.org/10.1186/s40659-015-0009-8
Tukappa N K A, et al. Cytotoxicity and Hepatoprotective Attributes of Methanolic Extract of Rumex Vesicarius L. Biol Res. 2015 Mar 25;48:19. PubMed PMID: 25857314.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L. AU - Tukappa N K,Asha, AU - Londonkar,Ramesh L, AU - Nayaka,Hanumantappa B, AU - Kumar C B,Sanjeev, Y1 - 2015/03/25/ PY - 2014/08/30/received PY - 2015/03/04/accepted PY - 2015/4/11/entrez PY - 2015/4/11/pubmed PY - 2015/10/16/medline SP - 19 EP - 19 JF - Biological research JO - Biol Res VL - 48 N2 - BACKGROUND: To evaluate the hepatoprotective potential and invitro cytotoxicity studies of whole plant methanol extract of Rumex vesicarius L. Methanol extract at a dose of 100 mg/kg bw and 200 mg/kg bw were assessed for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of SGOT (Serum glutamic oxaloacetic transaminase), SGPT (Serum glutamic pyruvic transaminase), ALP (Alkaline phosphatase), TP (Total protein), TB (Total bilirubin) and SOD (Superoxide dismutase), CAT (Catalase), MDA (Malondialdehyde). The cytotoxicity of the same extract on HepG2 cell lines were also assessed using MTT assay method at the concentration of 62.5, 125, 250, 500 μg/ml. RESULTS: Pretreatment of animals with whole plant methanol extracts of Rumex vesicarius L. significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver. The biochemical parameters in serum also improved in treated groups compared to the control and standard (silymarin) groups. Histopathological investigation further corroborated these biochemical observations. The cytotoxicity results indicated that the plant extract which were inhibitory to the proliferation of HepG2 cell line with IC50 value of 563.33 ± 0.8 μg/ml were not cytotoxic and appears to be safe. CONCLUSIONS: Rumex vesicarius L. whole plant methanol extract exhibit hepatoprotective activity. However the cytotoxicity in HepG2 is inexplicable and warrants further study. SN - 0717-6287 UR - https://www.unboundmedicine.com/medline/citation/25857314/Cytotoxicity_and_hepatoprotective_attributes_of_methanolic_extract_of_Rumex_vesicarius_L_ DB - PRIME DP - Unbound Medicine ER -