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The regulatory effects of Cissus quadrangularis on some enzymes involved in carbohydrate metabolism in streptozotocin-induced diabetic rats.
Pharm Biol. 2015 Aug; 53(8):1194-200.PB

Abstract

CONTEXT

Diabetes mellitus (DM) is probably the single most important metabolic disease and is widely recognized as one of the leading causes of death and disability. Cissus quadrangularis Linn. (Vitaceae) is a medicinal food and is reported to possess hypoglycemic activity.

OBJECTIVE

The present study evaluates the effect of ethyl acetate fraction of C. quadrangularis stem (CQSF) on carbohydrate metabolism in hepatic tissues of experimental diabetic rats. The phytochemical compounds present in the CQSF extract were identified by gas chromatography-mass spectrometry (GC-MS) analysis.

MATERIALS AND METHODS

Diabetic animals were treated with CQSF (50, 100, and 200 mg/kg body weight) for 45 d. Several indices such as blood glucose, glycated hemoglobin (HbA1c), insulin, liver function tests, hepatic glycogen content, and the activities of carbohydrate-metabolizing enzymes were assayed after 45 d of extract treatment.

RESULTS

A pronounced effect was observed with extract doses 100 and 200 mg/kg body weight. CQSF at a dose of 100 mg/kg body weight significantly decreased the altered levels of blood glucose by about 56%. CQSF also modulated the activities of carbohydrate-metabolizing enzymes by significantly increasing the activity of hexokinase (1.9-fold) and pyruvate kinase (2.2-fold) and significantly reducing the activity of glucose-6-phosphatase (41.23%), fructose-1,6-diphosphatase (29.43%), and glycogen phosphorylase (35.07%). GC-MS analysis revealed the presence of 10 chemical constituents, and N-methyl-1-adamantane acetamide was found to be the prevailing compound in the extract.

DISCUSSION AND CONCLUSION

The current study suggests the antidiabetic potential of CQSF, mediated through the regulation of carbohydrate metabolic enzyme activities.

Authors+Show Affiliations

Department of Biochemistry, University of Kerala , Kariavattom, Trivandrum, Kerala , India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25857823

Citation

Lekshmi, R K., et al. "The Regulatory Effects of Cissus Quadrangularis On some Enzymes Involved in Carbohydrate Metabolism in Streptozotocin-induced Diabetic Rats." Pharmaceutical Biology, vol. 53, no. 8, 2015, pp. 1194-200.
Lekshmi RK, Sreekutty MS, Mini S. The regulatory effects of Cissus quadrangularis on some enzymes involved in carbohydrate metabolism in streptozotocin-induced diabetic rats. Pharm Biol. 2015;53(8):1194-200.
Lekshmi, R. K., Sreekutty, M. S., & Mini, S. (2015). The regulatory effects of Cissus quadrangularis on some enzymes involved in carbohydrate metabolism in streptozotocin-induced diabetic rats. Pharmaceutical Biology, 53(8), 1194-200. https://doi.org/10.3109/13880209.2014.970286
Lekshmi RK, Sreekutty MS, Mini S. The Regulatory Effects of Cissus Quadrangularis On some Enzymes Involved in Carbohydrate Metabolism in Streptozotocin-induced Diabetic Rats. Pharm Biol. 2015;53(8):1194-200. PubMed PMID: 25857823.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The regulatory effects of Cissus quadrangularis on some enzymes involved in carbohydrate metabolism in streptozotocin-induced diabetic rats. AU - Lekshmi,R K, AU - Sreekutty,M S, AU - Mini,S, Y1 - 2015/04/10/ PY - 2015/4/11/entrez PY - 2015/4/11/pubmed PY - 2016/3/2/medline KW - Diabetes mellitus KW - GC–MS analysis KW - ethyl acetate fraction KW - hypoglycemic activity SP - 1194 EP - 200 JF - Pharmaceutical biology JO - Pharm Biol VL - 53 IS - 8 N2 - CONTEXT: Diabetes mellitus (DM) is probably the single most important metabolic disease and is widely recognized as one of the leading causes of death and disability. Cissus quadrangularis Linn. (Vitaceae) is a medicinal food and is reported to possess hypoglycemic activity. OBJECTIVE: The present study evaluates the effect of ethyl acetate fraction of C. quadrangularis stem (CQSF) on carbohydrate metabolism in hepatic tissues of experimental diabetic rats. The phytochemical compounds present in the CQSF extract were identified by gas chromatography-mass spectrometry (GC-MS) analysis. MATERIALS AND METHODS: Diabetic animals were treated with CQSF (50, 100, and 200 mg/kg body weight) for 45 d. Several indices such as blood glucose, glycated hemoglobin (HbA1c), insulin, liver function tests, hepatic glycogen content, and the activities of carbohydrate-metabolizing enzymes were assayed after 45 d of extract treatment. RESULTS: A pronounced effect was observed with extract doses 100 and 200 mg/kg body weight. CQSF at a dose of 100 mg/kg body weight significantly decreased the altered levels of blood glucose by about 56%. CQSF also modulated the activities of carbohydrate-metabolizing enzymes by significantly increasing the activity of hexokinase (1.9-fold) and pyruvate kinase (2.2-fold) and significantly reducing the activity of glucose-6-phosphatase (41.23%), fructose-1,6-diphosphatase (29.43%), and glycogen phosphorylase (35.07%). GC-MS analysis revealed the presence of 10 chemical constituents, and N-methyl-1-adamantane acetamide was found to be the prevailing compound in the extract. DISCUSSION AND CONCLUSION: The current study suggests the antidiabetic potential of CQSF, mediated through the regulation of carbohydrate metabolic enzyme activities. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25857823/The_regulatory_effects_of_Cissus_quadrangularis_on_some_enzymes_involved_in_carbohydrate_metabolism_in_streptozotocin_induced_diabetic_rats_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2014.970286 DB - PRIME DP - Unbound Medicine ER -