Tags

Type your tag names separated by a space and hit enter

Exposure medium: key in identifying free Ag+ as the exclusive species of silver nanoparticles with acute toxicity to Daphnia magna.
Sci Rep. 2015 Apr 10; 5:9674.SR

Abstract

It is still not very clear what roles the various Ag species play in the toxicity of silver nanoparticles (AgNPs). In this study, we found that traditional exposure media result in uncontrollable but consistent physicochemical transformation of AgNPs, causing artifacts in determination of median lethal concentration (LC50) and hindering the identification of Ag species responsible for the acute toxicity of AgNPs to Daphnia magna. This obstacle was overcome by using 8 h exposure in 0.1 mmol L(-1) NaNO3 medium, in which we measured the 8-h LC50 of seven AgNPs with different sizes and coatings, and determined the concentrations of various Ag species. The LC50 as free Ag(+) of the seven AgNPs (0.37-0.44 μg L(-1)) agreed very well with that of AgNO3 (0.40 μg L(-1)), and showed the lowest value compared to that as total Ag, total Ag(+), and dissolved Ag, demonstrating free Ag(+) is exclusively responsible for the acute toxicity of AgNPs to D. magna, while other Ag species in AgNPs have no contribution to the acute toxicity. Our results demonstrated the great importance of developing appropriate exposure media for evaluating risk of nanomaterials.

Authors+Show Affiliations

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P. O. Box 2871, Beijing 100085, China.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P. O. Box 2871, Beijing 100085, China.1] State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P. O. Box 2871, Beijing 100085, China [2] School of the Environment, Jiangsu University, Zhenjiang 212013, China.Chemical Metrology and Analytical Science Division, National Institute of Metrology, Beijing 100013, China.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P. O. Box 2871, Beijing 100085, China.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P. O. Box 2871, Beijing 100085, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25858866

Citation

Shen, Mo-Hai, et al. "Exposure Medium: Key in Identifying Free Ag+ as the Exclusive Species of Silver Nanoparticles With Acute Toxicity to Daphnia Magna." Scientific Reports, vol. 5, 2015, p. 9674.
Shen MH, Zhou XX, Yang XY, et al. Exposure medium: key in identifying free Ag+ as the exclusive species of silver nanoparticles with acute toxicity to Daphnia magna. Sci Rep. 2015;5:9674.
Shen, M. H., Zhou, X. X., Yang, X. Y., Chao, J. B., Liu, R., & Liu, J. F. (2015). Exposure medium: key in identifying free Ag+ as the exclusive species of silver nanoparticles with acute toxicity to Daphnia magna. Scientific Reports, 5, 9674. https://doi.org/10.1038/srep09674
Shen MH, et al. Exposure Medium: Key in Identifying Free Ag+ as the Exclusive Species of Silver Nanoparticles With Acute Toxicity to Daphnia Magna. Sci Rep. 2015 Apr 10;5:9674. PubMed PMID: 25858866.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exposure medium: key in identifying free Ag+ as the exclusive species of silver nanoparticles with acute toxicity to Daphnia magna. AU - Shen,Mo-Hai, AU - Zhou,Xiao-Xia, AU - Yang,Xiao-Ya, AU - Chao,Jing-Bo, AU - Liu,Rui, AU - Liu,Jing-Fu, Y1 - 2015/04/10/ PY - 2014/12/31/received PY - 2015/03/16/accepted PY - 2015/4/11/entrez PY - 2015/4/11/pubmed PY - 2016/1/7/medline SP - 9674 EP - 9674 JF - Scientific reports JO - Sci Rep VL - 5 N2 - It is still not very clear what roles the various Ag species play in the toxicity of silver nanoparticles (AgNPs). In this study, we found that traditional exposure media result in uncontrollable but consistent physicochemical transformation of AgNPs, causing artifacts in determination of median lethal concentration (LC50) and hindering the identification of Ag species responsible for the acute toxicity of AgNPs to Daphnia magna. This obstacle was overcome by using 8 h exposure in 0.1 mmol L(-1) NaNO3 medium, in which we measured the 8-h LC50 of seven AgNPs with different sizes and coatings, and determined the concentrations of various Ag species. The LC50 as free Ag(+) of the seven AgNPs (0.37-0.44 μg L(-1)) agreed very well with that of AgNO3 (0.40 μg L(-1)), and showed the lowest value compared to that as total Ag, total Ag(+), and dissolved Ag, demonstrating free Ag(+) is exclusively responsible for the acute toxicity of AgNPs to D. magna, while other Ag species in AgNPs have no contribution to the acute toxicity. Our results demonstrated the great importance of developing appropriate exposure media for evaluating risk of nanomaterials. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/25858866/Exposure_medium:_key_in_identifying_free_Ag+_as_the_exclusive_species_of_silver_nanoparticles_with_acute_toxicity_to_Daphnia_magna_ L2 - https://doi.org/10.1038/srep09674 DB - PRIME DP - Unbound Medicine ER -