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In vitro lethal effects of various extracts of Nigella sativa seed on hydatid cyst protoscoleces.

Abstract

OBJECTIVES

There are several scolicidal agents for inactivation of hydatid cyst protoscolices during surgery, but most of them are associated with adverse side effects such as sclerosing cholangitis and liver necrosis. The present study was aimed to evaluate scolicidal effects of various extracts of Nigella sativa seeds against protoscoleces of hydatid cyst in an in vitro model.

MATERIALS AND METHODS

Protoscoleces were aseptically aspirated from naturally infected livers of sheep and goats. Various concentrations of the different extracts of N. sativa (5 to 50 mg/ml) were used for 5 to 60 min. Viability of protoscoleces was confirmed by 0.1% eosin staining.

RESULTS

The findings exhibited that methanolic extract at the concentration of 50 mg/ml after 10 min of incubation, and aqueous extract at the concentration of 50 mg/ml after 30 min of incubation can kill 100% of protoscoleces. In addition, all of experiments revealed dose-dependent and also time-dependent scolicidal effect of various extracts of N. sativa on the protoscoleces of hydatid cyst.

CONCLUSION

The results of the present study demonstrated that N. sativa may be a natural source for the production of new scolicidal agent for use in hydatid cyst surgery. However, further studies will be required to evaluate scolicidal effects of N. sativa in the in vivo model.

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  • Authors+Show Affiliations

    ,

    Research Center for Tropical and Infectious Diseases, Kerman University of Medical Science, Kerman, Iran ; Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.

    ,

    Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences Kerman, Iran.

    ,

    Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.

    ,

    Department of Pharmacognosy, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.

    ,

    Research Center for Tropical and Infectious Diseases, Kerman University of Medical Science, Kerman, Iran ; Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.

    Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    25859304

    Citation

    Mahmoudvand, Hossein, et al. "In Vitro Lethal Effects of Various Extracts of Nigella Sativa Seed On Hydatid Cyst Protoscoleces." Iranian Journal of Basic Medical Sciences, vol. 17, no. 12, 2014, pp. 1001-6.
    Mahmoudvand H, Asadi A, Harandi MF, et al. In vitro lethal effects of various extracts of Nigella sativa seed on hydatid cyst protoscoleces. Iran J Basic Med Sci. 2014;17(12):1001-6.
    Mahmoudvand, H., Asadi, A., Harandi, M. F., Sharififar, F., Jahanbakhsh, S., & Dezaki, E. S. (2014). In vitro lethal effects of various extracts of Nigella sativa seed on hydatid cyst protoscoleces. Iranian Journal of Basic Medical Sciences, 17(12), pp. 1001-6.
    Mahmoudvand H, et al. In Vitro Lethal Effects of Various Extracts of Nigella Sativa Seed On Hydatid Cyst Protoscoleces. Iran J Basic Med Sci. 2014;17(12):1001-6. PubMed PMID: 25859304.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - In vitro lethal effects of various extracts of Nigella sativa seed on hydatid cyst protoscoleces. AU - Mahmoudvand,Hossein, AU - Asadi,Arash, AU - Harandi,Majid Fasihi, AU - Sharififar,Fariba, AU - Jahanbakhsh,Sareh, AU - Dezaki,Ebrahimm Saedi, PY - 2014/04/06/received PY - 2014/08/19/accepted PY - 2015/4/11/entrez PY - 2015/4/11/pubmed PY - 2015/4/11/medline KW - Echinococcus granulosus KW - Hydatid cyst KW - Nigella sativa SP - 1001 EP - 6 JF - Iranian journal of basic medical sciences JO - Iran J Basic Med Sci VL - 17 IS - 12 N2 - OBJECTIVES: There are several scolicidal agents for inactivation of hydatid cyst protoscolices during surgery, but most of them are associated with adverse side effects such as sclerosing cholangitis and liver necrosis. The present study was aimed to evaluate scolicidal effects of various extracts of Nigella sativa seeds against protoscoleces of hydatid cyst in an in vitro model. MATERIALS AND METHODS: Protoscoleces were aseptically aspirated from naturally infected livers of sheep and goats. Various concentrations of the different extracts of N. sativa (5 to 50 mg/ml) were used for 5 to 60 min. Viability of protoscoleces was confirmed by 0.1% eosin staining. RESULTS: The findings exhibited that methanolic extract at the concentration of 50 mg/ml after 10 min of incubation, and aqueous extract at the concentration of 50 mg/ml after 30 min of incubation can kill 100% of protoscoleces. In addition, all of experiments revealed dose-dependent and also time-dependent scolicidal effect of various extracts of N. sativa on the protoscoleces of hydatid cyst. CONCLUSION: The results of the present study demonstrated that N. sativa may be a natural source for the production of new scolicidal agent for use in hydatid cyst surgery. However, further studies will be required to evaluate scolicidal effects of N. sativa in the in vivo model. SN - 2008-3866 UR - https://www.unboundmedicine.com/medline/citation/25859304/In_vitro_lethal_effects_of_various_extracts_of_Nigella_sativa_seed_on_hydatid_cyst_protoscoleces_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25859304/ DB - PRIME DP - Unbound Medicine ER -