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Quantification of steroidal alkaloids in Buxus papillosa using electrospray ionization liquid chromatography-triple quadrupole mass spectrometry.
Steroids. 2015 Aug; 100:5-10.S

Abstract

Buxus papillosa is one of the most extensively studied species of the genus Buxus known to possess steroidal alkaloids which can be used for assessing the various pharmacological activities of this plant. This paper describes the liquid chromatography-electrospray ionization triple quadrupole mass spectrometry (LC-ESI-QQQ-MS) method for the quantification of six steroidal alkaloids as chemical markers in the extracts of leaves, roots and stems of B. papillosa. Quantitative MS/MS analysis was carried out by optimization of the most sensitive transition for each analyte. This has yielded detection and quantification limits of 0.486-8.08 ng/mL and 1.473-24.268 ng/mL, respectively for all analytes. Linearity of response was also achieved and the regression coefficient found to be >0.99 for all analyzed compounds. The newly developed MRM (Multiple Reaction Monitoring) method showed excellent sensitivity for the quantification of steroidal alkaloids within 15 min run time. This paper describes the application of LC-QQQ-MS technique for steroidal alkaloids analysis in plant samples.

Authors+Show Affiliations

H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan. Electronic address: musharraf1977@yahoo.com.H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25862613

Citation

Musharraf, Syed Ghulam, et al. "Quantification of Steroidal Alkaloids in Buxus Papillosa Using Electrospray Ionization Liquid Chromatography-triple Quadrupole Mass Spectrometry." Steroids, vol. 100, 2015, pp. 5-10.
Musharraf SG, Goher M, Zareena B. Quantification of steroidal alkaloids in Buxus papillosa using electrospray ionization liquid chromatography-triple quadrupole mass spectrometry. Steroids. 2015;100:5-10.
Musharraf, S. G., Goher, M., & Zareena, B. (2015). Quantification of steroidal alkaloids in Buxus papillosa using electrospray ionization liquid chromatography-triple quadrupole mass spectrometry. Steroids, 100, 5-10. https://doi.org/10.1016/j.steroids.2015.03.018
Musharraf SG, Goher M, Zareena B. Quantification of Steroidal Alkaloids in Buxus Papillosa Using Electrospray Ionization Liquid Chromatography-triple Quadrupole Mass Spectrometry. Steroids. 2015;100:5-10. PubMed PMID: 25862613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of steroidal alkaloids in Buxus papillosa using electrospray ionization liquid chromatography-triple quadrupole mass spectrometry. AU - Musharraf,Syed Ghulam, AU - Goher,Madiha, AU - Zareena,Bibi, Y1 - 2015/04/07/ PY - 2014/11/17/received PY - 2015/02/07/revised PY - 2015/03/31/accepted PY - 2015/4/12/entrez PY - 2015/4/12/pubmed PY - 2016/3/17/medline KW - Buxus papillosa KW - ESI-QQQ-MS/MS KW - Electrospray ionization mass spectrometry KW - Steroidal alkaloids SP - 5 EP - 10 JF - Steroids JO - Steroids VL - 100 N2 - Buxus papillosa is one of the most extensively studied species of the genus Buxus known to possess steroidal alkaloids which can be used for assessing the various pharmacological activities of this plant. This paper describes the liquid chromatography-electrospray ionization triple quadrupole mass spectrometry (LC-ESI-QQQ-MS) method for the quantification of six steroidal alkaloids as chemical markers in the extracts of leaves, roots and stems of B. papillosa. Quantitative MS/MS analysis was carried out by optimization of the most sensitive transition for each analyte. This has yielded detection and quantification limits of 0.486-8.08 ng/mL and 1.473-24.268 ng/mL, respectively for all analytes. Linearity of response was also achieved and the regression coefficient found to be >0.99 for all analyzed compounds. The newly developed MRM (Multiple Reaction Monitoring) method showed excellent sensitivity for the quantification of steroidal alkaloids within 15 min run time. This paper describes the application of LC-QQQ-MS technique for steroidal alkaloids analysis in plant samples. SN - 1878-5867 UR - https://www.unboundmedicine.com/medline/citation/25862613/Quantification_of_steroidal_alkaloids_in_Buxus_papillosa_using_electrospray_ionization_liquid_chromatography_triple_quadrupole_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-128X(15)00117-8 DB - PRIME DP - Unbound Medicine ER -