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Bi-functionalized aptasensor for ultrasensitive detection of thrombin.
Talanta. 2015 Jun 01; 138:273-278.T

Abstract

A novel bi-functionalized aptasensor for thrombin detection was fabricated by using electrochemiluminescence (ECL) and electrochemical impedance spectroscopy (EIS) techniques. A thiol-terminated aptamer with 15 oligonucleotides was hybridized with its complementary oligonucleotides to form a double-stranded DNA (dsDNA). Then, the thiol-labeled dsDNA was assembled on a Au electrode via Au-S bond; the other distal of the dsDNA labeled with biotin was bound to QDs through a biotin-avidin conjugation. When thrombin is present in the detection solution, the aptamer can combine with thrombin, resulting in loss of CdSe QDs from the electrode surface and thereby decreasing the ECL intensity and increasing the impedance. The decreased ECL and increased EIS signals are logarithmically linear with respect to the concentration of thrombin. The linear range was 10(-10)-10(-3) μg mL(-1) (R=0.9924) for the ECL signal and 10(-10)-10(-1) μg mL(-1) (R=0.9875) for the EIS method with a common detection limit of 10(-10) μg mL(-1) (2.7 aM). In addition, the bi-functionalized aptasensor exhibited excellent selectivity, super sensitivity, a low detection limit and a wide linear range.

Authors+Show Affiliations

Key Laboratory of Beijing on Regional Air Pollution Control, Beijing University of Technology, Beijing 100124, China. Electronic address: lipinglu@bjut.edu.cn.Key Laboratory of Beijing on Regional Air Pollution Control, Beijing University of Technology, Beijing 100124, China.Key Laboratory of Beijing on Regional Air Pollution Control, Beijing University of Technology, Beijing 100124, China.Key Laboratory of Beijing on Regional Air Pollution Control, Beijing University of Technology, Beijing 100124, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25863401

Citation

Lu, Liping, et al. "Bi-functionalized Aptasensor for Ultrasensitive Detection of Thrombin." Talanta, vol. 138, 2015, pp. 273-278.
Lu L, Li J, Kang T, et al. Bi-functionalized aptasensor for ultrasensitive detection of thrombin. Talanta. 2015;138:273-278.
Lu, L., Li, J., Kang, T., & Cheng, S. (2015). Bi-functionalized aptasensor for ultrasensitive detection of thrombin. Talanta, 138, 273-278. https://doi.org/10.1016/j.talanta.2015.03.016
Lu L, et al. Bi-functionalized Aptasensor for Ultrasensitive Detection of Thrombin. Talanta. 2015 Jun 1;138:273-278. PubMed PMID: 25863401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bi-functionalized aptasensor for ultrasensitive detection of thrombin. AU - Lu,Liping, AU - Li,Jiao, AU - Kang,Tianfang, AU - Cheng,Shuiyuan, Y1 - 2015/03/18/ PY - 2014/11/27/received PY - 2015/03/07/revised PY - 2015/03/10/accepted PY - 2015/4/13/entrez PY - 2015/4/13/pubmed PY - 2016/1/20/medline KW - Aptasensor KW - DNA KW - ECL KW - Quantum dots KW - Thrombin SP - 273 EP - 278 JF - Talanta JO - Talanta VL - 138 N2 - A novel bi-functionalized aptasensor for thrombin detection was fabricated by using electrochemiluminescence (ECL) and electrochemical impedance spectroscopy (EIS) techniques. A thiol-terminated aptamer with 15 oligonucleotides was hybridized with its complementary oligonucleotides to form a double-stranded DNA (dsDNA). Then, the thiol-labeled dsDNA was assembled on a Au electrode via Au-S bond; the other distal of the dsDNA labeled with biotin was bound to QDs through a biotin-avidin conjugation. When thrombin is present in the detection solution, the aptamer can combine with thrombin, resulting in loss of CdSe QDs from the electrode surface and thereby decreasing the ECL intensity and increasing the impedance. The decreased ECL and increased EIS signals are logarithmically linear with respect to the concentration of thrombin. The linear range was 10(-10)-10(-3) μg mL(-1) (R=0.9924) for the ECL signal and 10(-10)-10(-1) μg mL(-1) (R=0.9875) for the EIS method with a common detection limit of 10(-10) μg mL(-1) (2.7 aM). In addition, the bi-functionalized aptasensor exhibited excellent selectivity, super sensitivity, a low detection limit and a wide linear range. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/25863401/Bi_functionalized_aptasensor_for_ultrasensitive_detection_of_thrombin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(15)00169-1 DB - PRIME DP - Unbound Medicine ER -