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Antioxidant, antibacterial, and anti-inflammatory activities of standardized brazilin-rich Caesalpinia sappan extract.
Pharm Biol. 2015; 53(9):1339-43.PB

Abstract

CONTEXT

Brazilin is a major active principle of Caesalpinia sappan L. (Leguminosae or Fabaceae). For industry aspects, brazilin-rich extract (BRE) has been prepared and standardized to contain 39% w/w brazilin. BRE may have more advantages than brazilin in term of a lower-cost production process.

OBJECTIVES

To investigate the antioxidant, antibacterial, and anti-inflammatory activities of BRE.

MATERIAL AND METHODS

BRE was prepared by a simple one-step purification of the crude ethanol extract of C. sappan heartwood (CSE) using a Diaion® HP-20 column. The antioxidant activities were determined using three methods, including DPPH radical scavenging, reducing power, and β-carotene bleaching assays, at concentration ranges of 1-10, 10-100, and 10-100 µg/mL, respectively. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of BRE (15.6-1000 µg/mL) against Gram-positive and Gram-negative bacteria were determined by the broth microdilution method. Anti-inflammatory activity of BRE (0.1-5 µg/mL) was evaluated as anti-denaturation activity using bovine serum albumin as a substrate.

RESULTS AND DISCUSSION

On the basis of β-carotene bleaching assay, BRE showed antioxidant activity with an EC50 value of 60.5 µg/mL, which was almost equal to that of pure brazilin (52.1 µg/mL). Gram-positive bacteria were more sensitive to all tested samples than Gram-negative bacteria. BRE possessed higher antibacterial activities than CSE, but lower than brazilin. MIC/MBC values of 62.5-125/125 and 250-500/250-500 µg/mL were obtained for BRE against Gram-positive and Gram-negative bacteria, respectively. A low concentration (0.1 µg/mL) of brazilin, BRE, and CSE showed anti-inflammatory activity by inhibiting protein denaturation up to 46.8, 54.1, and 61.9%, respectively.

Authors+Show Affiliations

Excellent Research Laboratory, Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla Univesity, Hat-Yai, Songkhla , Thailand .No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25864864

Citation

Nirmal, Nilesh Prakash, and Pharkphoom Panichayupakaranant. "Antioxidant, Antibacterial, and Anti-inflammatory Activities of Standardized Brazilin-rich Caesalpinia Sappan Extract." Pharmaceutical Biology, vol. 53, no. 9, 2015, pp. 1339-43.
Nirmal NP, Panichayupakaranant P. Antioxidant, antibacterial, and anti-inflammatory activities of standardized brazilin-rich Caesalpinia sappan extract. Pharm Biol. 2015;53(9):1339-43.
Nirmal, N. P., & Panichayupakaranant, P. (2015). Antioxidant, antibacterial, and anti-inflammatory activities of standardized brazilin-rich Caesalpinia sappan extract. Pharmaceutical Biology, 53(9), 1339-43. https://doi.org/10.3109/13880209.2014.982295
Nirmal NP, Panichayupakaranant P. Antioxidant, Antibacterial, and Anti-inflammatory Activities of Standardized Brazilin-rich Caesalpinia Sappan Extract. Pharm Biol. 2015;53(9):1339-43. PubMed PMID: 25864864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant, antibacterial, and anti-inflammatory activities of standardized brazilin-rich Caesalpinia sappan extract. AU - Nirmal,Nilesh Prakash, AU - Panichayupakaranant,Pharkphoom, Y1 - 2015/04/13/ PY - 2015/4/14/entrez PY - 2015/4/14/pubmed PY - 2016/4/12/medline KW - Anti-denaturation KW - natural preservative KW - sappan wood SP - 1339 EP - 43 JF - Pharmaceutical biology JO - Pharm Biol VL - 53 IS - 9 N2 - CONTEXT: Brazilin is a major active principle of Caesalpinia sappan L. (Leguminosae or Fabaceae). For industry aspects, brazilin-rich extract (BRE) has been prepared and standardized to contain 39% w/w brazilin. BRE may have more advantages than brazilin in term of a lower-cost production process. OBJECTIVES: To investigate the antioxidant, antibacterial, and anti-inflammatory activities of BRE. MATERIAL AND METHODS: BRE was prepared by a simple one-step purification of the crude ethanol extract of C. sappan heartwood (CSE) using a Diaion® HP-20 column. The antioxidant activities were determined using three methods, including DPPH radical scavenging, reducing power, and β-carotene bleaching assays, at concentration ranges of 1-10, 10-100, and 10-100 µg/mL, respectively. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of BRE (15.6-1000 µg/mL) against Gram-positive and Gram-negative bacteria were determined by the broth microdilution method. Anti-inflammatory activity of BRE (0.1-5 µg/mL) was evaluated as anti-denaturation activity using bovine serum albumin as a substrate. RESULTS AND DISCUSSION: On the basis of β-carotene bleaching assay, BRE showed antioxidant activity with an EC50 value of 60.5 µg/mL, which was almost equal to that of pure brazilin (52.1 µg/mL). Gram-positive bacteria were more sensitive to all tested samples than Gram-negative bacteria. BRE possessed higher antibacterial activities than CSE, but lower than brazilin. MIC/MBC values of 62.5-125/125 and 250-500/250-500 µg/mL were obtained for BRE against Gram-positive and Gram-negative bacteria, respectively. A low concentration (0.1 µg/mL) of brazilin, BRE, and CSE showed anti-inflammatory activity by inhibiting protein denaturation up to 46.8, 54.1, and 61.9%, respectively. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25864864/Antioxidant_antibacterial_and_anti_inflammatory_activities_of_standardized_brazilin_rich_Caesalpinia_sappan_extract_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2014.982295 DB - PRIME DP - Unbound Medicine ER -