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Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients.
Andrology. 2015 May; 3(3):526-31.A

Abstract

The absence of the acrosome causes the situation which is called globozoospermia. There are a few studies, mostly as case reports, about correlation between levels of sperm DNA damage in patients with total round-headed spermatozoa. We investigated this correlation as well as CMA3 positive spermatozoa in 20 globozoospermic men (with more than 90% round-headed spermatozoa) attending to Royan Institute. Semen samples divided into three parts to semen analysis, to measure DNA fragmentation index (DFI) using sperm chromatin structure assay (SCSA) and to detect CMA3(+) sperm cells by chromomycin A3 staining and fluorescent microscopy. Our results showed that there were significant differences in sperm concentration, total sperm motility, and normal morphology between patients and controls group (p < 0.001). Moreover, the average of DFI and CMA3 positive spermatozoa in patients group significantly increases compared with control group (p < 0.001). A significant correlation between DFI and CMA3(+) in total population was also detected in patients group (r = 0.45, p = 0.046). To our knowledge, this is the largest study about correlation between DNA damage levels and CMA3 positive spermatozoa with round head sperm cells in total globozoospermic men. It seems that the increase in DNA damage may be because of defective sperm DNA compaction, as we detected CMA3 positive sperm cells in these patients.

Authors+Show Affiliations

Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25865619

Citation

Hosseinifar, H, et al. "Correlation Between Sperm DNA Fragmentation Index and CMA3 Positive Spermatozoa in Globozoospermic Patients." Andrology, vol. 3, no. 3, 2015, pp. 526-31.
Hosseinifar H, Yazdanikhah S, Modarresi T, et al. Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients. Andrology. 2015;3(3):526-31.
Hosseinifar, H., Yazdanikhah, S., Modarresi, T., Totonchi, M., Sadighi Gilani, M. A., & Sabbaghian, M. (2015). Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients. Andrology, 3(3), 526-31. https://doi.org/10.1111/andr.12030
Hosseinifar H, et al. Correlation Between Sperm DNA Fragmentation Index and CMA3 Positive Spermatozoa in Globozoospermic Patients. Andrology. 2015;3(3):526-31. PubMed PMID: 25865619.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients. AU - Hosseinifar,H, AU - Yazdanikhah,S, AU - Modarresi,T, AU - Totonchi,M, AU - Sadighi Gilani,M A, AU - Sabbaghian,M, Y1 - 2015/04/10/ PY - 2014/11/02/received PY - 2015/02/17/revised PY - 2015/02/18/accepted PY - 2015/4/14/entrez PY - 2015/4/14/pubmed PY - 2016/3/25/medline KW - DNA damage KW - chromomycinA3 KW - protamines KW - round head KW - spermatozoa SP - 526 EP - 31 JF - Andrology JO - Andrology VL - 3 IS - 3 N2 - The absence of the acrosome causes the situation which is called globozoospermia. There are a few studies, mostly as case reports, about correlation between levels of sperm DNA damage in patients with total round-headed spermatozoa. We investigated this correlation as well as CMA3 positive spermatozoa in 20 globozoospermic men (with more than 90% round-headed spermatozoa) attending to Royan Institute. Semen samples divided into three parts to semen analysis, to measure DNA fragmentation index (DFI) using sperm chromatin structure assay (SCSA) and to detect CMA3(+) sperm cells by chromomycin A3 staining and fluorescent microscopy. Our results showed that there were significant differences in sperm concentration, total sperm motility, and normal morphology between patients and controls group (p < 0.001). Moreover, the average of DFI and CMA3 positive spermatozoa in patients group significantly increases compared with control group (p < 0.001). A significant correlation between DFI and CMA3(+) in total population was also detected in patients group (r = 0.45, p = 0.046). To our knowledge, this is the largest study about correlation between DNA damage levels and CMA3 positive spermatozoa with round head sperm cells in total globozoospermic men. It seems that the increase in DNA damage may be because of defective sperm DNA compaction, as we detected CMA3 positive sperm cells in these patients. SN - 2047-2927 UR - https://www.unboundmedicine.com/medline/citation/25865619/Correlation_between_sperm_DNA_fragmentation_index_and_CMA3_positive_spermatozoa_in_globozoospermic_patients_ L2 - https://doi.org/10.1111/andr.12030 DB - PRIME DP - Unbound Medicine ER -