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G-CSF supports long-term muscle regeneration in mouse models of muscular dystrophy.
Nat Commun. 2015 Apr 13; 6:6745.NC

Abstract

Duchenne muscular dystrophy (DMD) is a chronic and life-threatening disease that is initially supported by muscle regeneration but eventually shows satellite cell exhaustion and muscular dysfunction. The life-long maintenance of skeletal muscle homoeostasis requires the satellite stem cell pool to be preserved. Asymmetric cell division plays a pivotal role in the maintenance of the satellite cell pool. Here we show that granulocyte colony-stimulating factor receptor (G-CSFR) is asymmetrically expressed in activated satellite cells. G-CSF positively affects the satellite cell population during multiple stages of differentiation in ex vivo cultured fibres. G-CSF could be important in developing an effective therapy for DMD based on its potential to modulate the supply of multiple stages of regenerated myocytes. This study shows that the G-CSF-G-CSFR axis is fundamentally important for long-term muscle regeneration, functional maintenance and lifespan extension in mouse models of DMD with varying severities.

Authors+Show Affiliations

Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.Department of Comparative and Experimental Medicine, Brain Research Institute, Niigata University, Niigata 951-8585, Japan.Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, Japan.Department of Cardiology, Keio University School of Medicine, 35-Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25865621

Citation

Hayashiji, Nozomi, et al. "G-CSF Supports Long-term Muscle Regeneration in Mouse Models of Muscular Dystrophy." Nature Communications, vol. 6, 2015, p. 6745.
Hayashiji N, Yuasa S, Miyagoe-Suzuki Y, et al. G-CSF supports long-term muscle regeneration in mouse models of muscular dystrophy. Nat Commun. 2015;6:6745.
Hayashiji, N., Yuasa, S., Miyagoe-Suzuki, Y., Hara, M., Ito, N., Hashimoto, H., Kusumoto, D., Seki, T., Tohyama, S., Kodaira, M., Kunitomi, A., Kashimura, S., Takei, M., Saito, Y., Okata, S., Egashira, T., Endo, J., Sasaoka, T., Takeda, S., & Fukuda, K. (2015). G-CSF supports long-term muscle regeneration in mouse models of muscular dystrophy. Nature Communications, 6, 6745. https://doi.org/10.1038/ncomms7745
Hayashiji N, et al. G-CSF Supports Long-term Muscle Regeneration in Mouse Models of Muscular Dystrophy. Nat Commun. 2015 Apr 13;6:6745. PubMed PMID: 25865621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - G-CSF supports long-term muscle regeneration in mouse models of muscular dystrophy. AU - Hayashiji,Nozomi, AU - Yuasa,Shinsuke, AU - Miyagoe-Suzuki,Yuko, AU - Hara,Mie, AU - Ito,Naoki, AU - Hashimoto,Hisayuki, AU - Kusumoto,Dai, AU - Seki,Tomohisa, AU - Tohyama,Shugo, AU - Kodaira,Masaki, AU - Kunitomi,Akira, AU - Kashimura,Shin, AU - Takei,Makoto, AU - Saito,Yuki, AU - Okata,Shinichiro, AU - Egashira,Toru, AU - Endo,Jin, AU - Sasaoka,Toshikuni, AU - Takeda,Shin'ichi, AU - Fukuda,Keiichi, Y1 - 2015/04/13/ PY - 2014/11/25/received PY - 2015/02/24/accepted PY - 2015/4/14/entrez PY - 2015/4/14/pubmed PY - 2016/1/9/medline SP - 6745 EP - 6745 JF - Nature communications JO - Nat Commun VL - 6 N2 - Duchenne muscular dystrophy (DMD) is a chronic and life-threatening disease that is initially supported by muscle regeneration but eventually shows satellite cell exhaustion and muscular dysfunction. The life-long maintenance of skeletal muscle homoeostasis requires the satellite stem cell pool to be preserved. Asymmetric cell division plays a pivotal role in the maintenance of the satellite cell pool. Here we show that granulocyte colony-stimulating factor receptor (G-CSFR) is asymmetrically expressed in activated satellite cells. G-CSF positively affects the satellite cell population during multiple stages of differentiation in ex vivo cultured fibres. G-CSF could be important in developing an effective therapy for DMD based on its potential to modulate the supply of multiple stages of regenerated myocytes. This study shows that the G-CSF-G-CSFR axis is fundamentally important for long-term muscle regeneration, functional maintenance and lifespan extension in mouse models of DMD with varying severities. SN - 2041-1723 UR - https://www.unboundmedicine.com/medline/citation/25865621/G_CSF_supports_long_term_muscle_regeneration_in_mouse_models_of_muscular_dystrophy_ L2 - https://doi.org/10.1038/ncomms7745 DB - PRIME DP - Unbound Medicine ER -