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High levels of DJ-1 protein and isoelectric point 6.3 isoform in sera of breast cancer patients.
Cancer Sci. 2015 Jul; 106(7):938-43.CS

Abstract

In patients with cancer and Parkinson's disease, the DJ-1 protein may be secreted into the serum during the impaired response of the underlying cell-protective mechanisms. In order to determine the clinical significance of DJ-1 protein in the sera of breast cancer patients, we examined blood samples from a breast cancer group (n = 180) and a non-cancerous control group (n = 300). Higher levels of DJ-1 were detected in the breast cancer group (mean level, 42.7 ng/mL) than the control group (28.3 ng/mL) by ELISA (P = 0.019). Higher DJ-1 levels were significantly associated with advanced clinical grade, according to the TNM classification, negative hormone receptor status, and high Ki-67 labeling index, of biopsied materials; samples showed low DJ-1 protein expression despite upregulated DJ-1 mRNA. DJ-1 isoforms could be detected clearly in 17 blood samples (from 11 breast cancer patients, and 6 non-cancerous controls) by 2-D gel electrophoresis and immunoblot analysis. The isoform at the pI of 6.3 showed the highest intensity in all 11 cancer cases. Conversely, in the 6 non-cancerous cases, isoforms other than the pI 6.3 isoform were highly expressed, and there was a significant difference in the isoform pattern between breast cancer cases and controls (P = 0.00025). These data indicate that high levels of DJ-1, probably of isoform at pI 6.3, is a candidate serum marker of breast cancer.

Authors+Show Affiliations

Department of Pathology, National Defense Medical College, Saitama, Japan. Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.Department of Pathology, National Defense Medical College, Saitama, Japan. Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.Department of Breast Surgery, Tokyo Medical University Ibaraki Medical Center, Ibaraki, Japan.Department of Surgery, National Defense Medical College, Saitama, Japan.Department of Surgery, National Defense Medical College, Saitama, Japan.Department of Surgery, National Defense Medical College, Saitama, Japan.Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.Department of Breast Surgery, Tokyo Medical University Ibaraki Medical Center, Ibaraki, Japan.Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College, Saitama, Japan.Laboratory for Mathematics, National Defense Medical College, Saitama, Japan.Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.Department of Pathology, National Defense Medical College, Saitama, Japan.Faculty of Agriculture, Hokkaido University, Sapporo, Japan.Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan.Kasumigaura Institute for Health Evaluation, Ibaraki, Japan.Department of Breast Surgery, Tokyo Medical University Ibaraki Medical Center, Ibaraki, Japan.Department of Surgery, National Defense Medical College, Saitama, Japan.Department of Pathology, National Defense Medical College, Saitama, Japan.Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25867058

Citation

Kawate, Takahiko, et al. "High Levels of DJ-1 Protein and Isoelectric Point 6.3 Isoform in Sera of Breast Cancer Patients." Cancer Science, vol. 106, no. 7, 2015, pp. 938-43.
Kawate T, Iwaya K, Koshikawa K, et al. High levels of DJ-1 protein and isoelectric point 6.3 isoform in sera of breast cancer patients. Cancer Sci. 2015;106(7):938-43.
Kawate, T., Iwaya, K., Koshikawa, K., Moriya, T., Yamasaki, T., Hasegawa, S., Kaise, H., Fujita, T., Matsuo, H., Nakamura, T., Ishikawa, T., Hiroi, S., Iguchi-Ariga, S. M., Ariga, H., Murota, K., Fujimori, M., Yamamoto, J., Matsubara, O., & Kohno, N. (2015). High levels of DJ-1 protein and isoelectric point 6.3 isoform in sera of breast cancer patients. Cancer Science, 106(7), 938-43. https://doi.org/10.1111/cas.12673
Kawate T, et al. High Levels of DJ-1 Protein and Isoelectric Point 6.3 Isoform in Sera of Breast Cancer Patients. Cancer Sci. 2015;106(7):938-43. PubMed PMID: 25867058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High levels of DJ-1 protein and isoelectric point 6.3 isoform in sera of breast cancer patients. AU - Kawate,Takahiko, AU - Iwaya,Keiichi, AU - Koshikawa,Kayoko, AU - Moriya,Tomoyuki, AU - Yamasaki,Tamio, AU - Hasegawa,Sho, AU - Kaise,Hiroshi, AU - Fujita,Tomoyuki, AU - Matsuo,Hirotaka, AU - Nakamura,Takahiro, AU - Ishikawa,Takashi, AU - Hiroi,Sadayuki, AU - Iguchi-Ariga,Sanae M M, AU - Ariga,Hiroyoshi, AU - Murota,Keiichi, AU - Fujimori,Minoru, AU - Yamamoto,Junji, AU - Matsubara,Osamu, AU - Kohno,Norio, Y1 - 2015/05/06/ PY - 2014/11/04/received PY - 2015/03/12/revised PY - 2015/04/08/accepted PY - 2015/4/14/entrez PY - 2015/4/14/pubmed PY - 2015/10/7/medline KW - 2-D gel electrophoresis KW - Biomarker KW - ELISA KW - Parkinson's disease KW - oxidative stress SP - 938 EP - 43 JF - Cancer science JO - Cancer Sci VL - 106 IS - 7 N2 - In patients with cancer and Parkinson's disease, the DJ-1 protein may be secreted into the serum during the impaired response of the underlying cell-protective mechanisms. In order to determine the clinical significance of DJ-1 protein in the sera of breast cancer patients, we examined blood samples from a breast cancer group (n = 180) and a non-cancerous control group (n = 300). Higher levels of DJ-1 were detected in the breast cancer group (mean level, 42.7 ng/mL) than the control group (28.3 ng/mL) by ELISA (P = 0.019). Higher DJ-1 levels were significantly associated with advanced clinical grade, according to the TNM classification, negative hormone receptor status, and high Ki-67 labeling index, of biopsied materials; samples showed low DJ-1 protein expression despite upregulated DJ-1 mRNA. DJ-1 isoforms could be detected clearly in 17 blood samples (from 11 breast cancer patients, and 6 non-cancerous controls) by 2-D gel electrophoresis and immunoblot analysis. The isoform at the pI of 6.3 showed the highest intensity in all 11 cancer cases. Conversely, in the 6 non-cancerous cases, isoforms other than the pI 6.3 isoform were highly expressed, and there was a significant difference in the isoform pattern between breast cancer cases and controls (P = 0.00025). These data indicate that high levels of DJ-1, probably of isoform at pI 6.3, is a candidate serum marker of breast cancer. SN - 1349-7006 UR - https://www.unboundmedicine.com/medline/citation/25867058/High_levels_of_DJ_1_protein_and_isoelectric_point_6_3_isoform_in_sera_of_breast_cancer_patients_ L2 - https://doi.org/10.1111/cas.12673 DB - PRIME DP - Unbound Medicine ER -