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Cytotoxic activity of Piper cubeba extract in breast cancer cell lines.
Nutrients. 2015 Apr 10; 7(4):2707-18.N

Abstract

This study aimed to evaluate the cytotoxicity of a crude extract of Piper cubeba against normal and breast cancer cell lines. To prepare the extract, P. cubeba seeds were ground, soaked in methanol and dichloromethane and isolated by column chromatography. Fractions were tested for cytotoxicity effects on normal fibroblast (L929), normal breast (MCF-12A) and breast cancer cell lines (MCF-7, MDA-MB-468 and MDA-MB-231). The most effective fraction was selected for DNA fragmentation assay to detect apoptotic activity. The results showed that the methanolic crude extract had a higher cytotoxic activity against MDA-MB-468 and MCF-7 than a dichloromethane crude extract. Then, the methanolic crude extract was separated into six fractions, designated A to F. Fraction C was highly active against breast cancer cell lines with an IC50 value less than 4 μg/mL. Therefore, Fraction C was further separated into seven fractions, CA to CG. The 1H-NMR profile showed that Fraction CE was long chain hydrocarbons. Moreover, Fraction CE demonstrated the highest activity against MCF-7 cells with an IC50 value of 2.69 ± 0.09 μg/mL and lower cytotoxicity against normal fibroblast L929 cells with an IC50 value of 4.17 ± 0.77 μg/mL. Finally, DNA fragmentation with a ladder pattern characteristic of apoptosis was observed in MCF-7, MDA-MB-468, MDA-MB-231 and L929 cells, but not in MCF-12A cells.

Authors+Show Affiliations

Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand. gpotchan@medicine.psu.ac.th. The Cancer Molecular Biology Excellence Research Laboratory, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand. gpotchan@medicine.psu.ac.th.Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand. mmananya@gmail.com.Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand. yaowapa.suk@psu.ac.th.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25867951

Citation

Graidist, Potchanapond, et al. "Cytotoxic Activity of Piper Cubeba Extract in Breast Cancer Cell Lines." Nutrients, vol. 7, no. 4, 2015, pp. 2707-18.
Graidist P, Martla M, Sukpondma Y. Cytotoxic activity of Piper cubeba extract in breast cancer cell lines. Nutrients. 2015;7(4):2707-18.
Graidist, P., Martla, M., & Sukpondma, Y. (2015). Cytotoxic activity of Piper cubeba extract in breast cancer cell lines. Nutrients, 7(4), 2707-18. https://doi.org/10.3390/nu7042707
Graidist P, Martla M, Sukpondma Y. Cytotoxic Activity of Piper Cubeba Extract in Breast Cancer Cell Lines. Nutrients. 2015 Apr 10;7(4):2707-18. PubMed PMID: 25867951.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxic activity of Piper cubeba extract in breast cancer cell lines. AU - Graidist,Potchanapond, AU - Martla,Mananya, AU - Sukpondma,Yaowapa, Y1 - 2015/04/10/ PY - 2015/01/23/received PY - 2015/03/20/revised PY - 2015/04/06/accepted PY - 2015/4/14/entrez PY - 2015/4/14/pubmed PY - 2016/1/31/medline SP - 2707 EP - 18 JF - Nutrients JO - Nutrients VL - 7 IS - 4 N2 - This study aimed to evaluate the cytotoxicity of a crude extract of Piper cubeba against normal and breast cancer cell lines. To prepare the extract, P. cubeba seeds were ground, soaked in methanol and dichloromethane and isolated by column chromatography. Fractions were tested for cytotoxicity effects on normal fibroblast (L929), normal breast (MCF-12A) and breast cancer cell lines (MCF-7, MDA-MB-468 and MDA-MB-231). The most effective fraction was selected for DNA fragmentation assay to detect apoptotic activity. The results showed that the methanolic crude extract had a higher cytotoxic activity against MDA-MB-468 and MCF-7 than a dichloromethane crude extract. Then, the methanolic crude extract was separated into six fractions, designated A to F. Fraction C was highly active against breast cancer cell lines with an IC50 value less than 4 μg/mL. Therefore, Fraction C was further separated into seven fractions, CA to CG. The 1H-NMR profile showed that Fraction CE was long chain hydrocarbons. Moreover, Fraction CE demonstrated the highest activity against MCF-7 cells with an IC50 value of 2.69 ± 0.09 μg/mL and lower cytotoxicity against normal fibroblast L929 cells with an IC50 value of 4.17 ± 0.77 μg/mL. Finally, DNA fragmentation with a ladder pattern characteristic of apoptosis was observed in MCF-7, MDA-MB-468, MDA-MB-231 and L929 cells, but not in MCF-12A cells. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/25867951/Cytotoxic_activity_of_Piper_cubeba_extract_in_breast_cancer_cell_lines_ L2 - https://www.mdpi.com/resolver?pii=nu7042707 DB - PRIME DP - Unbound Medicine ER -