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Assessment of hepatoprotective potential of N. indicum leaf on haloalkane xenobiotic induced hepatic injury in Swiss albino mice.
Chem Biol Interact. 2015 Jun 25; 235:37-46.CB

Abstract

CCl4 is a potent environmental toxin which cause liver damage through free radical mediated inflammatory processes. In this study, hepatoprotective capacity of Nerium indicum leaf extract (NILE) was evaluated on CCl4 induced acute hepatotoxicity in murine model. Animals were divided into 5 groups and treated as following: control group (received only normal saline), CCl4 group (received only CCl4), silymarin group (received CCl4 and 100mg/kg silymarin), NILE low group (received CCl4 and 50mg/kg NILE) and NILE high group (received CCl4 and 200mg/kg NILE). After 10 consecutive days of treatment, the levels of hepatic biochemical markers, malondialdehyde (MDA) content, peroxidase and catalase activities were measured as well as histopathological study was performed. Furthermore, liver explant cultures were set up as following: control (no treatment), CCl4 group (contained 25 μl/ml CCl4), silymarin group (contained 25 μl/ml CCl4 and 100 μg/ml silymarin), NILE low group (contained 25 μl/ml CCl4 and 25 μg/ml NILE) and NILE high group (contained 25 μl/ml CCl4 and 100 μg/ml NILE). Hepatic transaminases and phosphatases, tumor necrosis factor-α (TNF-α) expression, nitric oxide (NO) release and cell viability were studied on the explant cultures. Phytochemical fingerprinting of NILE was performed by gas chromatography-mass spectrometry (GC-MS). The results showed that the biochemical parameters were overexpressed due to CCl4 administration, which were significantly normalized by NILE treatment. The findings were further supported by histopathological evidences showing less hepatocellular necrosis, inflammation and fibrosis in NILE and silymarin treated groups, compared to CCl4 group. GC-MS analysis revealed presence of different bioactive phytochemicals with hepatoprotective and antioxidant properties. Therefore, the present study indicate that NILE possesses potent hepatoprotective capacity to ameliorate haloalkane xenobiotic induced injured liver in murine model.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Dey P
Cellular Immunology Laboratory, Department of Zoology, Life Science Building, University of North Bengal, PO: Raja Rammohunpur, Siliguri 734013, West Bengal, India. Electronic address: priyankardey28@gmail.com.
Dutta S
Cellular Immunology Laboratory, Department of Zoology, Life Science Building, University of North Bengal, PO: Raja Rammohunpur, Siliguri 734013, West Bengal, India. Electronic address: somitimmuno@gmail.com.
Sarkar MP
Chemical Signal and Lipidomics Laboratory, Department of Botany, University of Calcutta, Kolkata 700019, India. Electronic address: mousumipsarkar1@gmail.com.
Chaudhuri TK
Cellular Immunology Laboratory, Department of Zoology, Life Science Building, University of North Bengal, PO: Raja Rammohunpur, Siliguri 734013, West Bengal, India. Electronic address: dr_tkc_nbu@rediffmail.com.

MeSH

AnimalsAntioxidantsCarbon TetrachlorideCatalaseCell SurvivalChemical and Drug Induced Liver InjuryFemaleLiverLiver Function TestsMaleMalondialdehydeMiceNeriumNitric OxidePeroxidasePhosphoric Monoester HydrolasesPlant ExtractsPlant LeavesSilymarinTransaminasesTumor Necrosis Factor-alphaXenobiotics

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25871905

Citation

Dey, Priyankar, et al. "Assessment of Hepatoprotective Potential of N. Indicum Leaf On Haloalkane Xenobiotic Induced Hepatic Injury in Swiss Albino Mice." Chemico-biological Interactions, vol. 235, 2015, pp. 37-46.
Dey P, Dutta S, Sarkar MP, et al. Assessment of hepatoprotective potential of N. indicum leaf on haloalkane xenobiotic induced hepatic injury in Swiss albino mice. Chem Biol Interact. 2015;235:37-46.
Dey, P., Dutta, S., Sarkar, M. P., & Chaudhuri, T. K. (2015). Assessment of hepatoprotective potential of N. indicum leaf on haloalkane xenobiotic induced hepatic injury in Swiss albino mice. Chemico-biological Interactions, 235, 37-46. https://doi.org/10.1016/j.cbi.2015.03.025
Dey P, et al. Assessment of Hepatoprotective Potential of N. Indicum Leaf On Haloalkane Xenobiotic Induced Hepatic Injury in Swiss Albino Mice. Chem Biol Interact. 2015 Jun 25;235:37-46. PubMed PMID: 25871905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of hepatoprotective potential of N. indicum leaf on haloalkane xenobiotic induced hepatic injury in Swiss albino mice. AU - Dey,Priyankar, AU - Dutta,Somit, AU - Sarkar,Mousumi Poddar, AU - Chaudhuri,Tapas Kumar, Y1 - 2015/04/11/ PY - 2014/08/15/received PY - 2015/02/26/revised PY - 2015/03/22/accepted PY - 2015/4/15/entrez PY - 2015/4/15/pubmed PY - 2015/7/28/medline KW - Hepatoprotective KW - Lipid peroxidation KW - MTT KW - Nerium KW - Nitric oxide KW - TNF-α SP - 37 EP - 46 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 235 N2 - CCl4 is a potent environmental toxin which cause liver damage through free radical mediated inflammatory processes. In this study, hepatoprotective capacity of Nerium indicum leaf extract (NILE) was evaluated on CCl4 induced acute hepatotoxicity in murine model. Animals were divided into 5 groups and treated as following: control group (received only normal saline), CCl4 group (received only CCl4), silymarin group (received CCl4 and 100mg/kg silymarin), NILE low group (received CCl4 and 50mg/kg NILE) and NILE high group (received CCl4 and 200mg/kg NILE). After 10 consecutive days of treatment, the levels of hepatic biochemical markers, malondialdehyde (MDA) content, peroxidase and catalase activities were measured as well as histopathological study was performed. Furthermore, liver explant cultures were set up as following: control (no treatment), CCl4 group (contained 25 μl/ml CCl4), silymarin group (contained 25 μl/ml CCl4 and 100 μg/ml silymarin), NILE low group (contained 25 μl/ml CCl4 and 25 μg/ml NILE) and NILE high group (contained 25 μl/ml CCl4 and 100 μg/ml NILE). Hepatic transaminases and phosphatases, tumor necrosis factor-α (TNF-α) expression, nitric oxide (NO) release and cell viability were studied on the explant cultures. Phytochemical fingerprinting of NILE was performed by gas chromatography-mass spectrometry (GC-MS). The results showed that the biochemical parameters were overexpressed due to CCl4 administration, which were significantly normalized by NILE treatment. The findings were further supported by histopathological evidences showing less hepatocellular necrosis, inflammation and fibrosis in NILE and silymarin treated groups, compared to CCl4 group. GC-MS analysis revealed presence of different bioactive phytochemicals with hepatoprotective and antioxidant properties. Therefore, the present study indicate that NILE possesses potent hepatoprotective capacity to ameliorate haloalkane xenobiotic induced injured liver in murine model. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/25871905/Assessment_of_hepatoprotective_potential_of_N__indicum_leaf_on_haloalkane_xenobiotic_induced_hepatic_injury_in_Swiss_albino_mice_ DB - PRIME DP - Unbound Medicine ER -