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Diversity of Arbuscular Mycorrhizal Fungi Associated with a Sb Accumulator Plant, Ramie (Boehmeria nivea), in an Active Sb Mining.
J Microbiol Biotechnol. 2015 Aug; 25(8):1205-15.JM

Abstract

Arbuscular mycorrhizal fungi (AMF) have great potential for assisting heavy metal hyperaccumulators in the remediation of contaminated soils. However, little information is available about the symbiosis of AMF associated with an antimony (Sb) accumulator plant under natural conditions. Therefore, the objective of this study was to investigate the colonization and molecular diversity of AMF associated with the Sb accumulator ramie (Boehmeria nivea) growing in Sb-contaminated soils. Four Sb mine spoils and one adjacent reference area were selected from Xikuangshan in southern China. PCR-DGGE was used to analyze the AMF community composition in ramie roots. Morphological identification was also used to analyze the species in the rhizosphere soil of ramie. Results obtained showed that mycorrhizal symbiosis was established successfully even in the most heavily polluted sites. From the unpolluted site Ref to the highest polluted site T4, the spore numbers and AMF diversity increased at first and then decreased. Colonization increased consistently with the increasing Sb concentrations in the soil. A total of 14 species were identified by morphological analysis. From the total number of species, 4 (29%) belonged to Glomus, 2 (14%) belonged to Acaulospora, 2 (14%) belonged to Funneliformis, 1 (7%) belonged to Claroideoglomus, 1 (7%) belonged to Gigaspora, 1 (7%) belonged to Paraglomus, 1 (7%) belonging to Rhizophagus, 1 (7%) belonging to Sclervocystis, and 1 (7%) belonged to Scutellospora. Some AMF sequences were present even in the most polluted site. Morphological identification and phylogenetic analysis both revealed that most species were affiliated withGlomus, suggesting that Glomus was the dominant genus in this AMF community. This study demonstrated that ramie associated with AMF may have great potential for remediation of Sb-contaminated soils.

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Publisher Full Text (DOI)

Authors+Show Affiliations

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, P.R. China.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, P.R. China.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, P.R. China.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, P.R. China.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, P.R. China.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, P.R. China.

College of Resources and Environment, Hunan Agricultural University, Changsha 410128, P.R. China.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, P.R. China.

MeSH

AntimonyBiodiversityBoehmeriaChinaCluster AnalysisDNA, FungalDNA, RibosomalDenaturing Gradient Gel ElectrophoresisGenes, rRNAMolecular Sequence DataMycorrhizaePhylogenyPolymerase Chain ReactionRNA, FungalRNA, RibosomalRhizosphereSequence Analysis, DNASoil Microbiology

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25876600

Citation

Wei, Yuan, et al. "Diversity of Arbuscular Mycorrhizal Fungi Associated With a Sb Accumulator Plant, Ramie (Boehmeria Nivea), in an Active Sb Mining." Journal of Microbiology and Biotechnology, vol. 25, no. 8, 2015, pp. 1205-15.

Wei Y, Chen Z, Wu F, et al. Diversity of Arbuscular Mycorrhizal Fungi Associated with a Sb Accumulator Plant, Ramie (Boehmeria nivea), in an Active Sb Mining. J Microbiol Biotechnol. 2015;25(8):1205-15.

Wei, Y., Chen, Z., Wu, F., Li, J., ShangGuan, Y., Li, F., Zeng, Q. R., & Hou, H. (2015). Diversity of Arbuscular Mycorrhizal Fungi Associated with a Sb Accumulator Plant, Ramie (Boehmeria nivea), in an Active Sb Mining. Journal of Microbiology and Biotechnology, 25(8), 1205-15. https://doi.org/10.4014/jmb.1411.11033

Wei Y, et al. Diversity of Arbuscular Mycorrhizal Fungi Associated With a Sb Accumulator Plant, Ramie (Boehmeria Nivea), in an Active Sb Mining. J Microbiol Biotechnol. 2015;25(8):1205-15. PubMed PMID: 25876600.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Diversity of Arbuscular Mycorrhizal Fungi Associated with a Sb Accumulator Plant, Ramie (Boehmeria nivea), in an Active Sb Mining. AU - Wei,Yuan, AU - Chen,ZhiPeng, AU - Wu,FengChang, AU - Li,JiNing, AU - ShangGuan,YuXian, AU - Li,FaSheng, AU - Zeng,Qing Ru, AU - Hou,Hong, PY - 2015/4/17/entrez PY - 2015/4/17/pubmed PY - 2016/5/14/medline KW - antimony KW - arbuscular mycorrhizal fungi KW - phytoremediation KW - soil pollution SP - 1205 EP - 15 JF - Journal of microbiology and biotechnology JO - J Microbiol Biotechnol VL - 25 IS - 8 N2 - Arbuscular mycorrhizal fungi (AMF) have great potential for assisting heavy metal hyperaccumulators in the remediation of contaminated soils. However, little information is available about the symbiosis of AMF associated with an antimony (Sb) accumulator plant under natural conditions. Therefore, the objective of this study was to investigate the colonization and molecular diversity of AMF associated with the Sb accumulator ramie (Boehmeria nivea) growing in Sb-contaminated soils. Four Sb mine spoils and one adjacent reference area were selected from Xikuangshan in southern China. PCR-DGGE was used to analyze the AMF community composition in ramie roots. Morphological identification was also used to analyze the species in the rhizosphere soil of ramie. Results obtained showed that mycorrhizal symbiosis was established successfully even in the most heavily polluted sites. From the unpolluted site Ref to the highest polluted site T4, the spore numbers and AMF diversity increased at first and then decreased. Colonization increased consistently with the increasing Sb concentrations in the soil. A total of 14 species were identified by morphological analysis. From the total number of species, 4 (29%) belonged to Glomus, 2 (14%) belonged to Acaulospora, 2 (14%) belonged to Funneliformis, 1 (7%) belonged to Claroideoglomus, 1 (7%) belonged to Gigaspora, 1 (7%) belonged to Paraglomus, 1 (7%) belonging to Rhizophagus, 1 (7%) belonging to Sclervocystis, and 1 (7%) belonged to Scutellospora. Some AMF sequences were present even in the most polluted site. Morphological identification and phylogenetic analysis both revealed that most species were affiliated withGlomus, suggesting that Glomus was the dominant genus in this AMF community. This study demonstrated that ramie associated with AMF may have great potential for remediation of Sb-contaminated soils. SN - 1738-8872 UR - https://www.unboundmedicine.com/medline/citation/25876600/Diversity_of_Arbuscular_Mycorrhizal_Fungi_Associated_with_a_Sb_Accumulator_Plant_Ramie__Boehmeria_nivea__in_an_Active_Sb_Mining_ DB - PRIME DP - Unbound Medicine ER -