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Some biological activities of Epaltes divaricata L. - an in vitro study.
Ann Clin Microbiol Antimicrob. 2015 Mar 24; 14:18.AC

Abstract

BACKGROUND

Novel chemical molecules recovered from endangered medicinal plants have wide applications and have the potential to cure different diseases caused by microorganisms. The aim of this study was to investigate In vitro antimicrobial, α-glucosidase inhibition and antioxidant activity of different solvent extracts of Epaltes divaricata L.

METHODS

Antimicrobial activity of hexane, ethyl acetate and methanol extract of Epaltes divaricata was determined against bacteria and fungi using disc diffusion and microdilution method respectively. α-glucosidase inhibition, Total phenolic content (TPC), Reducing power activity, DPPH radical scavenging assay, hydroxyl radical scavenging activity, nitric oxide scavenging activity, superoxide scavenging activity and lipid peroxidation assay of plant extracts were performed according to standard protocol. Compound detection from the potential solvent extract was done through GC-MS analysis.

RESULTS

Epaltes divaricata ethyl acetate extracts (EDEa) (1.25 mg/disc) showed significant inhibition for E. lentum (23 mm), E. aerogenes (18 mm), P. fluorescence (15 mm) and A. baumanii (15 mm). Minimum inhibitory concentration (MIC) of EDEa was found to be 31.25 μg/ml, 62.5 μg/ml and 62.5 μg/ml against A. flavus, A. niger and T. rubrum respectively. EDEa showed more α-glucosidase inhibition and antioxidant activity compared to hexane and methanol. EDEa showed 50% α-glucosidase inhibition at the concentration of 525.20 ± 2.37 μg/ml. The TPC of EDEa was 412.0 ± 2.21 mg of catechol equivalents/g extract. EDEa showed great scavenging activity on 2,2-diphenyl-picrylhydrazyl (DPPH) (IC50 560 ± 2.02 μg/ml), hydroxyl (IC50 314.75 ± 2.56 μg/ml), nitric oxide (IC50 648.20 ± 2.09 μg/ml) and superoxide (IC50 361.14 ± 1.45 μg/ml) radicals, as well as high reducing power. EDEa also showed a more suppressive effect on lipid peroxidation. Using Antioxidant β-carotene linoleate method, the scavenging values of EDEa was significantly lower than BHT. GC-MS analysis of EDEa showed maximum amount of 2-butenamide, N-(4-fluorophenyl)-3-methyl trans-cinnamyl tiglate silane and trichlorocyclohexyl silane (36.86%).

CONCLUSION

The results obtained in this study clearly indicate that EDEa can be used as a natural antimicrobial, α-glucosidase inhibition and antioxidant agent.

Authors+Show Affiliations

Department of Botany, N.K.R. Govt. Arts College (W), Namakkal, 637 001, India. leelaglorybai@gmail.com.Department of Plant Biology and Biotechnology, Loyola College, Chennai, 600034, India. bharathikannan1210@gmail.com.Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia. mvalanarasu@gmail.com.Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia. naldhabi@ksu.edu.sa.Department of Plant Biology and Biotechnology, Loyola College, Chennai, 600034, India. agastian@loyolacollege.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25879935

Citation

Glorybai, Leela, et al. "Some Biological Activities of Epaltes Divaricata L. - an in Vitro Study." Annals of Clinical Microbiology and Antimicrobials, vol. 14, 2015, p. 18.
Glorybai L, Kannan K B, Arasu MV, et al. Some biological activities of Epaltes divaricata L. - an in vitro study. Ann Clin Microbiol Antimicrob. 2015;14:18.
Glorybai, L., Kannan K, B., Arasu, M. V., Al-Dhabi, N. A., & Agastian, P. (2015). Some biological activities of Epaltes divaricata L. - an in vitro study. Annals of Clinical Microbiology and Antimicrobials, 14, 18. https://doi.org/10.1186/s12941-015-0074-4
Glorybai L, et al. Some Biological Activities of Epaltes Divaricata L. - an in Vitro Study. Ann Clin Microbiol Antimicrob. 2015 Mar 24;14:18. PubMed PMID: 25879935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Some biological activities of Epaltes divaricata L. - an in vitro study. AU - Glorybai,Leela, AU - Kannan K,Barathi, AU - Arasu,Mariadhas Valan, AU - Al-Dhabi,Naif Abdullah, AU - Agastian,Paul, Y1 - 2015/03/24/ PY - 2015/01/07/received PY - 2015/03/05/accepted PY - 2015/4/17/entrez PY - 2015/4/17/pubmed PY - 2015/12/22/medline SP - 18 EP - 18 JF - Annals of clinical microbiology and antimicrobials JO - Ann Clin Microbiol Antimicrob VL - 14 N2 - BACKGROUND: Novel chemical molecules recovered from endangered medicinal plants have wide applications and have the potential to cure different diseases caused by microorganisms. The aim of this study was to investigate In vitro antimicrobial, α-glucosidase inhibition and antioxidant activity of different solvent extracts of Epaltes divaricata L. METHODS: Antimicrobial activity of hexane, ethyl acetate and methanol extract of Epaltes divaricata was determined against bacteria and fungi using disc diffusion and microdilution method respectively. α-glucosidase inhibition, Total phenolic content (TPC), Reducing power activity, DPPH radical scavenging assay, hydroxyl radical scavenging activity, nitric oxide scavenging activity, superoxide scavenging activity and lipid peroxidation assay of plant extracts were performed according to standard protocol. Compound detection from the potential solvent extract was done through GC-MS analysis. RESULTS: Epaltes divaricata ethyl acetate extracts (EDEa) (1.25 mg/disc) showed significant inhibition for E. lentum (23 mm), E. aerogenes (18 mm), P. fluorescence (15 mm) and A. baumanii (15 mm). Minimum inhibitory concentration (MIC) of EDEa was found to be 31.25 μg/ml, 62.5 μg/ml and 62.5 μg/ml against A. flavus, A. niger and T. rubrum respectively. EDEa showed more α-glucosidase inhibition and antioxidant activity compared to hexane and methanol. EDEa showed 50% α-glucosidase inhibition at the concentration of 525.20 ± 2.37 μg/ml. The TPC of EDEa was 412.0 ± 2.21 mg of catechol equivalents/g extract. EDEa showed great scavenging activity on 2,2-diphenyl-picrylhydrazyl (DPPH) (IC50 560 ± 2.02 μg/ml), hydroxyl (IC50 314.75 ± 2.56 μg/ml), nitric oxide (IC50 648.20 ± 2.09 μg/ml) and superoxide (IC50 361.14 ± 1.45 μg/ml) radicals, as well as high reducing power. EDEa also showed a more suppressive effect on lipid peroxidation. Using Antioxidant β-carotene linoleate method, the scavenging values of EDEa was significantly lower than BHT. GC-MS analysis of EDEa showed maximum amount of 2-butenamide, N-(4-fluorophenyl)-3-methyl trans-cinnamyl tiglate silane and trichlorocyclohexyl silane (36.86%). CONCLUSION: The results obtained in this study clearly indicate that EDEa can be used as a natural antimicrobial, α-glucosidase inhibition and antioxidant agent. SN - 1476-0711 UR - https://www.unboundmedicine.com/medline/citation/25879935/Some_biological_activities_of_Epaltes_divaricata_L____an_in_vitro_study_ L2 - https://ann-clinmicrob.biomedcentral.com/articles/10.1186/s12941-015-0074-4 DB - PRIME DP - Unbound Medicine ER -