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Comparative Study of the Biological Activity of Allantoin and Aqueous Extract of the Comfrey Root.
Phytother Res 2015; 29(8):1117-22PR

Abstract

This study investigates the biological activity of pure allantoin (PA) and aqueous extract of the comfrey (Symphytum officinale L.) root (AECR) standardized to the allantoin content. Cell viability and proliferation of epithelial (MDCK) and fibroblastic (L929) cell line were studied by using MTT test. Anti-irritant potential was determined by measuring electrical capacitance, erythema index (EI) and transepidermal water loss of artificially irritated skin of young healthy volunteers, 3 and 7 days after application of creams and gels with PA or AECR. Pure allantoin showed mild inhibitory effect on proliferation of both cell lines at concentrations 40 and 100 µg/ml, but more pronounced on MDCK cells. Aqueous extract of the comfrey root effect on cell proliferation in concentrations higher than 40 µg/ml was significantly stimulatory for L929 but inhibitory for MDCK cells. Pharmaceutical preparations that contained AECR showed better anti-irritant potential compared with PA. Creams showed better effect on hydration and EI compared with the gels that contained the same components. Our results indicate that the biological activity of the comfrey root extract cannot be attributed only to allantoin but is also likely the result of the interaction of different compounds present in AECR. Topical preparations that contain comfrey extract may have a great application in the treatment of skin irritation.

Authors+Show Affiliations

University of Niš, Department for Pharmacy, Faculty of Medicine, Blvd. Dr Zoran Djindjić 81, 18000 Niš, Serbia.University of Niš, Faculty of Technology, Bulevar oslobodjenja 124, Leskovac, Serbia.University of Niš, Department for Pharmacy, Faculty of Medicine, Blvd. Dr Zoran Djindjić 81, 18000 Niš, Serbia.University of Niš, Faculty of Technology, Bulevar oslobodjenja 124, Leskovac, Serbia.University of Niš, Department for Cell and Tissue Engineering, Institute of Biology and Human Genetics, Faculty of Medicine, Blvd. Dr Zoran Djindjić 81, 18000 Niš, Serbia.University of Niš, Department for Cell and Tissue Engineering, Institute of Biology and Human Genetics, Faculty of Medicine, Blvd. Dr Zoran Djindjić 81, 18000 Niš, Serbia.University of Niš, Faculty of Technology, Bulevar oslobodjenja 124, Leskovac, Serbia.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25880800

Citation

Savić, Vesna Lj, et al. "Comparative Study of the Biological Activity of Allantoin and Aqueous Extract of the Comfrey Root." Phytotherapy Research : PTR, vol. 29, no. 8, 2015, pp. 1117-22.
Savić VLj, Nikolić VD, Arsić IA, et al. Comparative Study of the Biological Activity of Allantoin and Aqueous Extract of the Comfrey Root. Phytother Res. 2015;29(8):1117-22.
Savić, V. L. j., Nikolić, V. D., Arsić, I. A., Stanojević, L. P., Najman, S. J., Stojanović, S., & Mladenović-Ranisavljević, I. I. (2015). Comparative Study of the Biological Activity of Allantoin and Aqueous Extract of the Comfrey Root. Phytotherapy Research : PTR, 29(8), pp. 1117-22. doi:10.1002/ptr.5356.
Savić VLj, et al. Comparative Study of the Biological Activity of Allantoin and Aqueous Extract of the Comfrey Root. Phytother Res. 2015;29(8):1117-22. PubMed PMID: 25880800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative Study of the Biological Activity of Allantoin and Aqueous Extract of the Comfrey Root. AU - Savić,Vesna Lj, AU - Nikolić,Vesna D, AU - Arsić,Ivana A, AU - Stanojević,Ljiljana P, AU - Najman,Stevo J, AU - Stojanović,Sanja, AU - Mladenović-Ranisavljević,Ivana I, Y1 - 2015/04/16/ PY - 2014/09/13/received PY - 2015/03/18/revised PY - 2015/03/22/accepted PY - 2015/4/17/entrez PY - 2015/4/17/pubmed PY - 2015/12/15/medline KW - allantoin KW - anti-irritant potential KW - comfrey KW - extract KW - proliferation KW - viability SP - 1117 EP - 22 JF - Phytotherapy research : PTR JO - Phytother Res VL - 29 IS - 8 N2 - This study investigates the biological activity of pure allantoin (PA) and aqueous extract of the comfrey (Symphytum officinale L.) root (AECR) standardized to the allantoin content. Cell viability and proliferation of epithelial (MDCK) and fibroblastic (L929) cell line were studied by using MTT test. Anti-irritant potential was determined by measuring electrical capacitance, erythema index (EI) and transepidermal water loss of artificially irritated skin of young healthy volunteers, 3 and 7 days after application of creams and gels with PA or AECR. Pure allantoin showed mild inhibitory effect on proliferation of both cell lines at concentrations 40 and 100 µg/ml, but more pronounced on MDCK cells. Aqueous extract of the comfrey root effect on cell proliferation in concentrations higher than 40 µg/ml was significantly stimulatory for L929 but inhibitory for MDCK cells. Pharmaceutical preparations that contained AECR showed better anti-irritant potential compared with PA. Creams showed better effect on hydration and EI compared with the gels that contained the same components. Our results indicate that the biological activity of the comfrey root extract cannot be attributed only to allantoin but is also likely the result of the interaction of different compounds present in AECR. Topical preparations that contain comfrey extract may have a great application in the treatment of skin irritation. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/25880800/Comparative_Study_of_the_Biological_Activity_of_Allantoin_and_Aqueous_Extract_of_the_Comfrey_Root_ L2 - https://doi.org/10.1002/ptr.5356 DB - PRIME DP - Unbound Medicine ER -