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The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model.
BMC Complement Altern Med. 2015 Apr 15; 15:119.BC

Abstract

BACKGROUND

Ginsenoside Rg1 (Rg1) is one of the most active ingredients in Panax ginseng and has been proven to have anti-oxidative and anti-aging properties. However, there have been few reports concerning the anti-aging effects of Rg1 on the hematopoietic microenvironment and bone marrow stromal cells (BMSCs).

METHODS

Thirty Sprague-Dawley rats were randomly divided into four groups (control, D-galactose (D-gal)-administration, Rg1-treatment, and D-gal-administration + Rg1-treatment groups). After D-gal and Rg1 treatment, BMSCs were extracted from femoral bone marrow for culture. After three passages, BMSCs were tested by senescence-associated β-galactosidase (SA-β-gal) staining, flow cytometric cell cycle phase distribution assay, CCK-8 cell proliferation assay, oxidative stress (reactive oxygen species [ROS], superoxide dismutase [SOD], and malondialdehyde [MDA]) assays, inflammatory marker (interleukin (IL)-2, IL-6, and tumor necrosis factor (TNF)-α) enzyme-linked immunosorbent assay (ELISA), stem cell factor (SCF) ELISA, and senescence-associated protein (p16, p21, and p53) Western blotting.

RESULTS

Compared to the D-gal-administration group, the D-gal-administration + Rg1-treatment group showed significantly decreased levels of SA-β-gal + cell %, ROS, MDA, inflammatory marker expression, and senescence-associated protein expression as well as significantly increased levels of S-phase %, cell proliferation, SOD activity, and SCF expression. Compared to controls, the Rg-1-treatment group displayed significantly reduced levels of SA-β-gal + cell %, G1 phase %, ROS, MDA, inflammatory marker expression, senescence-associated protein expression, and SCF expression as well as significantly increased levels of S-phase %, cell proliferation, and SOD activity.

CONCLUSIONS

Rg1 improves the anti-aging ability of hematopoietic microenvironment through enhancing the anti-oxidant and anti-inflammatory capacities of BMSCs.

Authors+Show Affiliations

Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China. sorocker@163.com. Department of stomatology and oral & maxillofacial surgery, YongChuan Hospital, Chongqing Medical University, 402160, Chongqing, China. sorocker@163.com.Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China. 150136487@qq.com.Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China. 76893096@qq.com.Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China. yanyan_zhangcq@163.com.Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China. jdy_cq023@163.com.Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China. yaping_wang023@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25881060

Citation

Hu, Wenxu, et al. "The Positive Effects of Ginsenoside Rg1 Upon the Hematopoietic Microenvironment in a D-Galactose-induced Aged Rat Model." BMC Complementary and Alternative Medicine, vol. 15, 2015, p. 119.
Hu W, Jing P, Wang L, et al. The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model. BMC Complement Altern Med. 2015;15:119.
Hu, W., Jing, P., Wang, L., Zhang, Y., Yong, J., & Wang, Y. (2015). The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model. BMC Complementary and Alternative Medicine, 15, 119. https://doi.org/10.1186/s12906-015-0642-3
Hu W, et al. The Positive Effects of Ginsenoside Rg1 Upon the Hematopoietic Microenvironment in a D-Galactose-induced Aged Rat Model. BMC Complement Altern Med. 2015 Apr 15;15:119. PubMed PMID: 25881060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model. AU - Hu,Wenxu, AU - Jing,Pengwei, AU - Wang,Lu, AU - Zhang,Yanyan, AU - Yong,Jiadao, AU - Wang,Yaping, Y1 - 2015/04/15/ PY - 2014/11/03/received PY - 2015/04/07/accepted PY - 2015/4/17/entrez PY - 2015/4/17/pubmed PY - 2015/12/15/medline SP - 119 EP - 119 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 15 N2 - BACKGROUND: Ginsenoside Rg1 (Rg1) is one of the most active ingredients in Panax ginseng and has been proven to have anti-oxidative and anti-aging properties. However, there have been few reports concerning the anti-aging effects of Rg1 on the hematopoietic microenvironment and bone marrow stromal cells (BMSCs). METHODS: Thirty Sprague-Dawley rats were randomly divided into four groups (control, D-galactose (D-gal)-administration, Rg1-treatment, and D-gal-administration + Rg1-treatment groups). After D-gal and Rg1 treatment, BMSCs were extracted from femoral bone marrow for culture. After three passages, BMSCs were tested by senescence-associated β-galactosidase (SA-β-gal) staining, flow cytometric cell cycle phase distribution assay, CCK-8 cell proliferation assay, oxidative stress (reactive oxygen species [ROS], superoxide dismutase [SOD], and malondialdehyde [MDA]) assays, inflammatory marker (interleukin (IL)-2, IL-6, and tumor necrosis factor (TNF)-α) enzyme-linked immunosorbent assay (ELISA), stem cell factor (SCF) ELISA, and senescence-associated protein (p16, p21, and p53) Western blotting. RESULTS: Compared to the D-gal-administration group, the D-gal-administration + Rg1-treatment group showed significantly decreased levels of SA-β-gal + cell %, ROS, MDA, inflammatory marker expression, and senescence-associated protein expression as well as significantly increased levels of S-phase %, cell proliferation, SOD activity, and SCF expression. Compared to controls, the Rg-1-treatment group displayed significantly reduced levels of SA-β-gal + cell %, G1 phase %, ROS, MDA, inflammatory marker expression, senescence-associated protein expression, and SCF expression as well as significantly increased levels of S-phase %, cell proliferation, and SOD activity. CONCLUSIONS: Rg1 improves the anti-aging ability of hematopoietic microenvironment through enhancing the anti-oxidant and anti-inflammatory capacities of BMSCs. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/25881060/The_positive_effects_of_Ginsenoside_Rg1_upon_the_hematopoietic_microenvironment_in_a_D_Galactose_induced_aged_rat_model_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-015-0642-3 DB - PRIME DP - Unbound Medicine ER -