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TOPK is highly expressed in circulating tumor cells, enabling metastasis of prostate cancer.
Oncotarget 2015; 6(14):12392-404O

Abstract

Circulating tumor cells (CTCs) are important for metastasis in prostate cancer. T-LAK cell-originated protein kinase (TOPK) is highly expressed in cancer cells. Herein, we established a xenograft animal model, isolated and cultured the CTCs, and found CTCs have significantly greater migratory capacity than parental cells. TOPK is more highly expressed in the CTCs than in parental cells and is also highly expressed in the metastatic nodules caused by CTCs in mice. Knocking down TOPK decreased the migration of CTCs both in vitro and in vivo. TOPK was modulated by the PI3K/PTEN and ERK pathways during the metastasis of prostate cancer. High levels of TOPK in the tumors of patients were correlated with advanced stages of prostate cancer, especially for high-risk patients of Gleason score≥8, PSA>20ng/ml. In summary, TOPK was speculated to be one of a potential marker and therapeutic target in advanced prostate cancer.

Authors+Show Affiliations

Department of Urology, Xijing Hospital, The Fourth Military Medical University, Xian, China.Department of Epidemiology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xian, China.Laboratory Animal Center, The Fourth Military Medical University, Xian, Shaanxi, China.Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xian, China.Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xian, China.Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xian, China.Department of Biochemistry and Molecular Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, China.Department of Biochemistry, Department of Basic Medicine, The Fourth Military Medical University, Xian, China.PLA Lhasa General Hospital, Lhasa, China.Department of Biochemistry and Molecular Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, China.Department of Biochemistry and Molecular Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, China.Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xian, China.Department of Biochemistry and Molecular Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, China.Department of Urology, Xijing Hospital, The Fourth Military Medical University, Xian, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25881543

Citation

Sun, Huimin, et al. "TOPK Is Highly Expressed in Circulating Tumor Cells, Enabling Metastasis of Prostate Cancer." Oncotarget, vol. 6, no. 14, 2015, pp. 12392-404.
Sun H, Zhang L, Shi C, et al. TOPK is highly expressed in circulating tumor cells, enabling metastasis of prostate cancer. Oncotarget. 2015;6(14):12392-404.
Sun, H., Zhang, L., Shi, C., Hu, P., Yan, W., Wang, Z., ... Shao, C. (2015). TOPK is highly expressed in circulating tumor cells, enabling metastasis of prostate cancer. Oncotarget, 6(14), pp. 12392-404.
Sun H, et al. TOPK Is Highly Expressed in Circulating Tumor Cells, Enabling Metastasis of Prostate Cancer. Oncotarget. 2015 May 20;6(14):12392-404. PubMed PMID: 25881543.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TOPK is highly expressed in circulating tumor cells, enabling metastasis of prostate cancer. AU - Sun,Huimin, AU - Zhang,Lei, AU - Shi,Changhong, AU - Hu,Peizhen, AU - Yan,Wei, AU - Wang,Zhe, AU - Duan,Qiuhong, AU - Lu,Fan, AU - Qin,Lipeng, AU - Lu,Tao, AU - Xiao,Juanjuan, AU - Wang,Yingmei, AU - Zhu,Feng, AU - Shao,Chen, PY - 2014/12/13/received PY - 2015/02/26/accepted PY - 2015/4/18/entrez PY - 2015/4/18/pubmed PY - 2016/3/26/medline KW - T-LAK cell-originated protein kinase (TOPK) KW - circulating tumor cells KW - metastasis KW - prostate cancer SP - 12392 EP - 404 JF - Oncotarget JO - Oncotarget VL - 6 IS - 14 N2 - Circulating tumor cells (CTCs) are important for metastasis in prostate cancer. T-LAK cell-originated protein kinase (TOPK) is highly expressed in cancer cells. Herein, we established a xenograft animal model, isolated and cultured the CTCs, and found CTCs have significantly greater migratory capacity than parental cells. TOPK is more highly expressed in the CTCs than in parental cells and is also highly expressed in the metastatic nodules caused by CTCs in mice. Knocking down TOPK decreased the migration of CTCs both in vitro and in vivo. TOPK was modulated by the PI3K/PTEN and ERK pathways during the metastasis of prostate cancer. High levels of TOPK in the tumors of patients were correlated with advanced stages of prostate cancer, especially for high-risk patients of Gleason score≥8, PSA>20ng/ml. In summary, TOPK was speculated to be one of a potential marker and therapeutic target in advanced prostate cancer. SN - 1949-2553 UR - https://www.unboundmedicine.com/medline/citation/25881543/TOPK_is_highly_expressed_in_circulating_tumor_cells_enabling_metastasis_of_prostate_cancer_ L2 - http://www.impactjournals.com/oncotarget/misc/linkedout.php?pii=3630 DB - PRIME DP - Unbound Medicine ER -