Tags

Type your tag names separated by a space and hit enter

Analysis of phytohormones in vermicompost using a novel combinative sample preparation strategy of ultrasound-assisted extraction and solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry.
Talanta. 2015 Jul 01; 139:189-97.T

Abstract

Vermicompost (VC), a widely used premium organic fertilizer, is the by-product of symbiotic interactions between earthworms and microorganisms living within them. It has been postulated that phytohormones are plausible "magic compounds" in VC that are responsible for making them such good fertilizers. Thus, a novel approach involving ultrasound-assisted extraction (UAE) and solid-phase extraction (SPE) was developed as a fast and efficient sample preparation method to screen for different classes of phytohormones in VC by liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis. Nine phytohormones from three different classes, including trans-zeatin (tZ), kinetin (K), N(6)-[2-isopentyl]adenine (iP), N(6)-benzyladenine (BA), N(6)-isopentenyladenosine (iPR), indole-3-acetic acid (IAA), 4-[3-indolyl]butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and (+)-abscisic acid (ABA), were simultaneously screened. The extraction parameters influencing UAE efficiency were optimized to provide comparable recovery to the conventional mix-stirring (MSt) method. The optimized UAE method was subsequently applied on the analysis of phytohormones in VC, i.e. phytohormone extract was further pre-concentrated and purified using C18 and MCX SPE cartridges prior to LC-MS/MS analysis. The following phytohormones, namely iP, iPR and IAA, were detected and quantified to be 0.49, 0.53, 79.78ngg(-1), respectively; tZ was found to be below the limit of quantitation. Recoveries of 10.2%, 9.1%, 18.9% and 0.3% for tZ, iP, iPR and IAA were obtained. This is one of the few reported works for the successful detection and quantitation of cytokinins and auxins in VC, that provided the key empirical evidence to explain the growth efficacy of applying VC in promoting plant growth. Additionally, this pioneering work could potentially be applicable for the analysis of other types of organic fertilizers such as composts and activated composted materials awaiting phytohormone analyzes for quality assessment and control.

Authors+Show Affiliations

Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore; Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.Natural Sciences and Science Education Academic Group, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore.School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.Nanyang Environment & Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, CleanTech One, #06-08, Singapore 637141, Singapore.College of Life Science, Zhejiang University, Hangzhou 310058, China.Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore. Electronic address: jyong@sutd.edu.sg.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25882426

Citation

Zhang, Hong, et al. "Analysis of Phytohormones in Vermicompost Using a Novel Combinative Sample Preparation Strategy of Ultrasound-assisted Extraction and Solid-phase Extraction Coupled With Liquid Chromatography-tandem Mass Spectrometry." Talanta, vol. 139, 2015, pp. 189-97.
Zhang H, Tan SN, Teo CH, et al. Analysis of phytohormones in vermicompost using a novel combinative sample preparation strategy of ultrasound-assisted extraction and solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry. Talanta. 2015;139:189-97.
Zhang, H., Tan, S. N., Teo, C. H., Yew, Y. R., Ge, L., Chen, X., & Yong, J. W. (2015). Analysis of phytohormones in vermicompost using a novel combinative sample preparation strategy of ultrasound-assisted extraction and solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry. Talanta, 139, 189-97. https://doi.org/10.1016/j.talanta.2015.02.052
Zhang H, et al. Analysis of Phytohormones in Vermicompost Using a Novel Combinative Sample Preparation Strategy of Ultrasound-assisted Extraction and Solid-phase Extraction Coupled With Liquid Chromatography-tandem Mass Spectrometry. Talanta. 2015 Jul 1;139:189-97. PubMed PMID: 25882426.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of phytohormones in vermicompost using a novel combinative sample preparation strategy of ultrasound-assisted extraction and solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry. AU - Zhang,Hong, AU - Tan,Swee Ngin, AU - Teo,Chee How, AU - Yew,Yan Ru, AU - Ge,Liya, AU - Chen,Xin, AU - Yong,Jean Wan Hong, Y1 - 2015/03/06/ PY - 2014/12/06/received PY - 2015/02/11/revised PY - 2015/02/15/accepted PY - 2015/4/18/entrez PY - 2015/4/18/pubmed PY - 2016/1/23/medline KW - Auxins KW - Cytokinins KW - Liquid chromatography–tandem mass spectrometry KW - Phytohormones KW - Solid-phase extraction KW - Ultrasound-assisted extraction KW - Vermicompost SP - 189 EP - 97 JF - Talanta JO - Talanta VL - 139 N2 - Vermicompost (VC), a widely used premium organic fertilizer, is the by-product of symbiotic interactions between earthworms and microorganisms living within them. It has been postulated that phytohormones are plausible "magic compounds" in VC that are responsible for making them such good fertilizers. Thus, a novel approach involving ultrasound-assisted extraction (UAE) and solid-phase extraction (SPE) was developed as a fast and efficient sample preparation method to screen for different classes of phytohormones in VC by liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis. Nine phytohormones from three different classes, including trans-zeatin (tZ), kinetin (K), N(6)-[2-isopentyl]adenine (iP), N(6)-benzyladenine (BA), N(6)-isopentenyladenosine (iPR), indole-3-acetic acid (IAA), 4-[3-indolyl]butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and (+)-abscisic acid (ABA), were simultaneously screened. The extraction parameters influencing UAE efficiency were optimized to provide comparable recovery to the conventional mix-stirring (MSt) method. The optimized UAE method was subsequently applied on the analysis of phytohormones in VC, i.e. phytohormone extract was further pre-concentrated and purified using C18 and MCX SPE cartridges prior to LC-MS/MS analysis. The following phytohormones, namely iP, iPR and IAA, were detected and quantified to be 0.49, 0.53, 79.78ngg(-1), respectively; tZ was found to be below the limit of quantitation. Recoveries of 10.2%, 9.1%, 18.9% and 0.3% for tZ, iP, iPR and IAA were obtained. This is one of the few reported works for the successful detection and quantitation of cytokinins and auxins in VC, that provided the key empirical evidence to explain the growth efficacy of applying VC in promoting plant growth. Additionally, this pioneering work could potentially be applicable for the analysis of other types of organic fertilizers such as composts and activated composted materials awaiting phytohormone analyzes for quality assessment and control. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/25882426/Analysis_of_phytohormones_in_vermicompost_using_a_novel_combinative_sample_preparation_strategy_of_ultrasound_assisted_extraction_and_solid_phase_extraction_coupled_with_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(15)00134-4 DB - PRIME DP - Unbound Medicine ER -