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Solanum lycopersicum AUXIN RESPONSE FACTOR 9 regulates cell division activity during early tomato fruit development.
J Exp Bot. 2015 Jun; 66(11):3405-16.JE

Abstract

The transformation of the ovary into a fruit after successful completion of pollination and fertilization has been associated with many changes at transcriptomic level. These changes are part of a dynamic and complex regulatory network that is controlled by phytohormones, with a major role for auxin. One of the auxin-related genes differentially expressed upon fruit set and early fruit development in tomato is Solanum lycopersicum AUXIN RESPONSE FACTOR 9 (SlARF9). Here, the functional analysis of this ARF is described. SlARF9 expression was found to be auxin-responsive and SlARF9 mRNA levels were high in the ovules, placenta, and pericarp of pollinated ovaries, but also in other plant tissues with high cell division activity, such as the axillary meristems and root meristems. Transgenic plants with increased SlARF9 mRNA levels formed fruits that were smaller than wild-type fruits because of reduced cell division activity, whereas transgenic lines in which SlARF9 mRNA levels were reduced showed the opposite phenotype. The expression analysis, together with the phenotype of the transgenic lines, suggests that, in tomato, ARF9 negatively controls cell division during early fruit development.

Authors+Show Affiliations

Department of Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.Department of Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.Department of Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.Department of Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.Department of Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.Computational and Systems Biology, Rothamsted Research, West Common, Harpenden, Hertfordshire, AL5 2JQ, UK.Plant Research International, Wageningen University & Research Plant Breeding, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.Plant Research International, Wageningen University & Research Plant Breeding, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.Department of Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.Department of Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.Department of Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands I.Rieu@science.ru.nl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25883382

Citation

de Jong, Maaike, et al. "Solanum Lycopersicum AUXIN RESPONSE FACTOR 9 Regulates Cell Division Activity During Early Tomato Fruit Development." Journal of Experimental Botany, vol. 66, no. 11, 2015, pp. 3405-16.
de Jong M, Wolters-Arts M, Schimmel BC, et al. Solanum lycopersicum AUXIN RESPONSE FACTOR 9 regulates cell division activity during early tomato fruit development. J Exp Bot. 2015;66(11):3405-16.
de Jong, M., Wolters-Arts, M., Schimmel, B. C., Stultiens, C. L., de Groot, P. F., Powers, S. J., Tikunov, Y. M., Bovy, A. G., Mariani, C., Vriezen, W. H., & Rieu, I. (2015). Solanum lycopersicum AUXIN RESPONSE FACTOR 9 regulates cell division activity during early tomato fruit development. Journal of Experimental Botany, 66(11), 3405-16. https://doi.org/10.1093/jxb/erv152
de Jong M, et al. Solanum Lycopersicum AUXIN RESPONSE FACTOR 9 Regulates Cell Division Activity During Early Tomato Fruit Development. J Exp Bot. 2015;66(11):3405-16. PubMed PMID: 25883382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solanum lycopersicum AUXIN RESPONSE FACTOR 9 regulates cell division activity during early tomato fruit development. AU - de Jong,Maaike, AU - Wolters-Arts,Mieke, AU - Schimmel,Bernardus C J, AU - Stultiens,Catharina L M, AU - de Groot,Peter F M, AU - Powers,Stephen J, AU - Tikunov,Yury M, AU - Bovy,Arnoud G, AU - Mariani,Celestina, AU - Vriezen,Wim H, AU - Rieu,Ivo, Y1 - 2015/04/16/ PY - 2015/4/18/entrez PY - 2015/4/18/pubmed PY - 2016/3/31/medline KW - AUXIN RESPONSE FACTOR 9 (ARF9) KW - Auxin KW - cell division KW - fruit development KW - fruit size KW - tomato (Solanum lycopersicum L.). SP - 3405 EP - 16 JF - Journal of experimental botany JO - J Exp Bot VL - 66 IS - 11 N2 - The transformation of the ovary into a fruit after successful completion of pollination and fertilization has been associated with many changes at transcriptomic level. These changes are part of a dynamic and complex regulatory network that is controlled by phytohormones, with a major role for auxin. One of the auxin-related genes differentially expressed upon fruit set and early fruit development in tomato is Solanum lycopersicum AUXIN RESPONSE FACTOR 9 (SlARF9). Here, the functional analysis of this ARF is described. SlARF9 expression was found to be auxin-responsive and SlARF9 mRNA levels were high in the ovules, placenta, and pericarp of pollinated ovaries, but also in other plant tissues with high cell division activity, such as the axillary meristems and root meristems. Transgenic plants with increased SlARF9 mRNA levels formed fruits that were smaller than wild-type fruits because of reduced cell division activity, whereas transgenic lines in which SlARF9 mRNA levels were reduced showed the opposite phenotype. The expression analysis, together with the phenotype of the transgenic lines, suggests that, in tomato, ARF9 negatively controls cell division during early fruit development. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/25883382/Solanum_lycopersicum_AUXIN_RESPONSE_FACTOR_9_regulates_cell_division_activity_during_early_tomato_fruit_development_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erv152 DB - PRIME DP - Unbound Medicine ER -