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A novel multiplex assay for simultaneously analysing 13 rapidly mutating Y-STRs.
Forensic Sci Int Genet. 2015 Jul; 17:91-98.FS

Abstract

A multiplex polymerase chain reaction (PCR) assay (RM-Yplex) was developed which is capable of simultaneously amplifying 13 recently introduced rapidly mutating Y-STR markers (RM Y-STRs). This multiplex assay is expected to aid human identity testing in forensic and other applications to improve differentiating unrelated males and allow separating related males. The 13 RM Y-STR markers included in the multiplex are: DYF387S1, DYF399S1, DYF403S1ab, DYF404S1, DYS449, DYS518, DYS526ab, DYS547, DYS570, DYS576, DYS612, DYS626 and DYS627. This study reflects the proof of concept to analyse all currently known RM Y-STRs simultaneously and describes the optimization of the multiplex assay. The RM-Yplex assay generated complete RM Y-STR profiles down to 62.5pg of male template DNA, and from male-female DNA mixtures at all ratios tested. We herewith introduce and make available for widespread use in forensic and anthropological studies, an effective and sensitive single multiplex assay for simultaneous genotyping of 13 RM Y-STRs.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Alghafri R
University of Central Lancashire, School of Forensic and Investigative Sciences, Preston, UK; General Department of Forensic Sciences and Criminology, Dubai Police General Head Quarters, Dubai, United Arab Emirates.
Goodwin W
University of Central Lancashire, School of Forensic and Investigative Sciences, Preston, UK.
Ralf A
Department of Forensic Molecular Biology, Erasmus MC University Medical Centre Rotterdam, Rotterdam, The Netherlands.
Kayser M
Department of Forensic Molecular Biology, Erasmus MC University Medical Centre Rotterdam, Rotterdam, The Netherlands.
Hadi S
University of Central Lancashire, School of Forensic and Investigative Sciences, Preston, UK. Electronic address: shadi@uclan.ac.uk.

MeSH

AllelesChromosomes, Human, YForensic GeneticsHaplotypesHumansMaleMicrosatellite RepeatsMultiplex Polymerase Chain ReactionMutation

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25884342

Citation

Alghafri, Rashed, et al. "A Novel Multiplex Assay for Simultaneously Analysing 13 Rapidly Mutating Y-STRs." Forensic Science International. Genetics, vol. 17, 2015, pp. 91-98.
Alghafri R, Goodwin W, Ralf A, et al. A novel multiplex assay for simultaneously analysing 13 rapidly mutating Y-STRs. Forensic Sci Int Genet. 2015;17:91-98.
Alghafri, R., Goodwin, W., Ralf, A., Kayser, M., & Hadi, S. (2015). A novel multiplex assay for simultaneously analysing 13 rapidly mutating Y-STRs. Forensic Science International. Genetics, 17, 91-98. https://doi.org/10.1016/j.fsigen.2015.04.004
Alghafri R, et al. A Novel Multiplex Assay for Simultaneously Analysing 13 Rapidly Mutating Y-STRs. Forensic Sci Int Genet. 2015;17:91-98. PubMed PMID: 25884342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel multiplex assay for simultaneously analysing 13 rapidly mutating Y-STRs. AU - Alghafri,Rashed, AU - Goodwin,Will, AU - Ralf,Arwin, AU - Kayser,Manfred, AU - Hadi,Sibte, Y1 - 2015/04/09/ PY - 2014/11/24/received PY - 2015/03/26/revised PY - 2015/04/07/accepted PY - 2015/4/18/entrez PY - 2015/4/18/pubmed PY - 2016/4/19/medline KW - Haplotypes KW - Rapidly mutating Y-STRs KW - Y-STRs KW - Y-chromosomal STRs SP - 91 EP - 98 JF - Forensic science international. Genetics JO - Forensic Sci Int Genet VL - 17 N2 - A multiplex polymerase chain reaction (PCR) assay (RM-Yplex) was developed which is capable of simultaneously amplifying 13 recently introduced rapidly mutating Y-STR markers (RM Y-STRs). This multiplex assay is expected to aid human identity testing in forensic and other applications to improve differentiating unrelated males and allow separating related males. The 13 RM Y-STR markers included in the multiplex are: DYF387S1, DYF399S1, DYF403S1ab, DYF404S1, DYS449, DYS518, DYS526ab, DYS547, DYS570, DYS576, DYS612, DYS626 and DYS627. This study reflects the proof of concept to analyse all currently known RM Y-STRs simultaneously and describes the optimization of the multiplex assay. The RM-Yplex assay generated complete RM Y-STR profiles down to 62.5pg of male template DNA, and from male-female DNA mixtures at all ratios tested. We herewith introduce and make available for widespread use in forensic and anthropological studies, an effective and sensitive single multiplex assay for simultaneous genotyping of 13 RM Y-STRs. SN - 1878-0326 UR - https://www.unboundmedicine.com/medline/citation/25884342/A_novel_multiplex_assay_for_simultaneously_analysing_13_rapidly_mutating_Y_STRs_ DB - PRIME DP - Unbound Medicine ER -