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In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica.
Biol Res. 2015 Mar 14; 48:15.BR

Abstract

BACKGROUND

Excessive production of free radicals causes direct damage to biological molecules such as DNA, proteins, lipids, carbohydrates leading to tumor development and progression. Natural antioxidant molecules from phytochemicals of plant origin may directly inhibit either their production or limit their propagation or destroy them to protect the system. In the present study, Monodora myristica a non-timber forest product consumed in Cameroon as spice was screened for its free radical scavenging properties, antioxidant and enzymes protective activities. Its phenolic compound profile was also realized by HPLC.

RESULTS

This study demonstrated that M. myristica has scavenging properties against DPPH(•), OH(•), NO(•), and ABTS(•) radicals which vary in a dose depending manner. It also showed an antioxidant potential that was comparable with that of Butylated Hydroxytoluene (BHT) and vitamin C used as standard. The aqueous ethanol extract of M. myristica barks (AEH); showed a significantly higher content in polyphenolic compounds (21.44 ± 0.24 mg caffeic acid/g dried extract) and flavonoid (5.69 ± 0.07 quercetin equivalent mg/g of dried weight) as compared to the other studied extracts. The HPLC analysis of the barks and leaves revealed the presence of several polyphenols. The acids (3,4-OH-benzoic, caffeic, gallic, O- and P- coumaric, syringic, vanillic), alcohols (tyrosol and OH-tyrosol), theobromine, quercetin, rutin, catechine and apigenin were the identified and quantified polyphenols. All the tested extracts demonstrated a high protective potential on the superoxide dismutase (SOD), catalase and peroxidase activities.

CONCLUSION

Finally, the different extracts from M. myristica and specifically the aqueous ethanol extract reveal several properties such as higher free radical scavenging properties, significant antioxidant capacities and protective potential effects on liver enzymes.

Authors+Show Affiliations

Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences; Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaounde, Cameroon. mouket2006@yahoo.fr.Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences; Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaounde, Cameroon. apieme@yahoo.fr.Department of Inorganic Chemistry, Faculty of Sciences, University of Yaoundé I, P.O Box 812, Yaoundé, Cameroon. jnjimou@yahoo.fr. Department of Chemical Materials Environmental Engineering, Via Eudossiana 18, University of Rome "La Sapienza", Rome, Italy. jnjimou@yahoo.fr.Laboratory of Medicinal plant Biochemistry, Food Science and Nutrition, Department of Biochemistry, Faculty of Science, University of Dschang, PO Box: 67, Dschang, Cameroon. brbiapa@yahoo.fr.Department of Chemical Materials Environmental Engineering, Via Eudossiana 18, University of Rome "La Sapienza", Rome, Italy. marco.bravi@uniroma1.it.Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences; Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaounde, Cameroon. jngogang@yahoo.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25885269

Citation

Moukette, Bruno Moukette, et al. "In Vitro Antioxidant Properties, Free Radicals Scavenging Activities of Extracts and Polyphenol Composition of a Non-timber Forest Product Used as Spice: Monodora Myristica." Biological Research, vol. 48, 2015, p. 15.
Moukette BM, Pieme CA, Njimou JR, et al. In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica. Biol Res. 2015;48:15.
Moukette, B. M., Pieme, C. A., Njimou, J. R., Biapa, C. P., Marco, B., & Ngogang, J. Y. (2015). In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica. Biological Research, 48, 15. https://doi.org/10.1186/s40659-015-0003-1
Moukette BM, et al. In Vitro Antioxidant Properties, Free Radicals Scavenging Activities of Extracts and Polyphenol Composition of a Non-timber Forest Product Used as Spice: Monodora Myristica. Biol Res. 2015 Mar 14;48:15. PubMed PMID: 25885269.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica. AU - Moukette,Bruno Moukette, AU - Pieme,Constant Anatole, AU - Njimou,Jacques Romain, AU - Biapa,Cabral Prosper Nya, AU - Marco,Bravi, AU - Ngogang,Jeanne Yonkeu, Y1 - 2015/03/14/ PY - 2014/09/04/received PY - 2015/02/11/accepted PY - 2015/4/18/entrez PY - 2015/4/18/pubmed PY - 2015/10/16/medline SP - 15 EP - 15 JF - Biological research JO - Biol Res VL - 48 N2 - BACKGROUND: Excessive production of free radicals causes direct damage to biological molecules such as DNA, proteins, lipids, carbohydrates leading to tumor development and progression. Natural antioxidant molecules from phytochemicals of plant origin may directly inhibit either their production or limit their propagation or destroy them to protect the system. In the present study, Monodora myristica a non-timber forest product consumed in Cameroon as spice was screened for its free radical scavenging properties, antioxidant and enzymes protective activities. Its phenolic compound profile was also realized by HPLC. RESULTS: This study demonstrated that M. myristica has scavenging properties against DPPH(•), OH(•), NO(•), and ABTS(•) radicals which vary in a dose depending manner. It also showed an antioxidant potential that was comparable with that of Butylated Hydroxytoluene (BHT) and vitamin C used as standard. The aqueous ethanol extract of M. myristica barks (AEH); showed a significantly higher content in polyphenolic compounds (21.44 ± 0.24 mg caffeic acid/g dried extract) and flavonoid (5.69 ± 0.07 quercetin equivalent mg/g of dried weight) as compared to the other studied extracts. The HPLC analysis of the barks and leaves revealed the presence of several polyphenols. The acids (3,4-OH-benzoic, caffeic, gallic, O- and P- coumaric, syringic, vanillic), alcohols (tyrosol and OH-tyrosol), theobromine, quercetin, rutin, catechine and apigenin were the identified and quantified polyphenols. All the tested extracts demonstrated a high protective potential on the superoxide dismutase (SOD), catalase and peroxidase activities. CONCLUSION: Finally, the different extracts from M. myristica and specifically the aqueous ethanol extract reveal several properties such as higher free radical scavenging properties, significant antioxidant capacities and protective potential effects on liver enzymes. SN - 0717-6287 UR - https://www.unboundmedicine.com/medline/citation/25885269/In_vitro_antioxidant_properties_free_radicals_scavenging_activities_of_extracts_and_polyphenol_composition_of_a_non_timber_forest_product_used_as_spice:_Monodora_myristica_ L2 - https://biolres.biomedcentral.com/articles/10.1186/s40659-015-0003-1 DB - PRIME DP - Unbound Medicine ER -