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Changes of cytokine levels in a mouse model of post-infectious irritable bowel syndrome.
BMC Gastroenterol. 2015 Apr 01; 15:43.BG

Abstract

BACKGROUND

Irritable bowel syndrome (IBS) is a highly prevalent functional gastrointestinal disorder. Post-infectious IBS (PI-IBS) is caused by an acute gastrointestinal infection preceding the onset of symptoms. However, the pathophysiology of PI-IBS is not clear, and the purpose of this study was to investigate the probable immune mechanisms of PI-IBS.

METHODS

C57BL/6 mice were randomly assigned to either an infection group or a control group. Mice in the infection group were infected with Trichinella spiralis to establish a model of PI-IBS (500 Trichinella), while control mice received only salt solution. Visceral sensitivity of colorectal distention in mice was evaluated by abdominal withdrawal reflex scores and intestinal inflammation was assessed using hematoxylin-eosin staining; at day 56 post-infection, the mRNA and protein levels of specific cytokines in the gut segments were detected using reverse-transcription polymerase chain reaction and enzyme-linked immunoabsorbent assay.

RESULTS

Levels of interferon γ and interleukin (IL)-17 in the PI-IBS group were significantly increased in the duodenum and ileum, and IL-10 was decreased in the jejunum, ileum, and colon compared with control mice. However, the expression level of IL-1β was not significantly different between the two groups.

CONCLUSIONS

The present study suggests that the local low-grade inflammation and immune activation that are an important component of the pathophysiology of PI-IBS are primarily induced and maintained by specific cytokines.

Authors+Show Affiliations

Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 4000 l6, China. yblyl9527@126.com.Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 4000 l6, China. chqxchzh@163.com.Department of Gastroenterology, Hainan Provincial People's Hospital, Haikou, 570311, China. yangbo7552@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25886744

Citation

Yang, Bo, et al. "Changes of Cytokine Levels in a Mouse Model of Post-infectious Irritable Bowel Syndrome." BMC Gastroenterology, vol. 15, 2015, p. 43.
Yang B, Zhou X, Lan C. Changes of cytokine levels in a mouse model of post-infectious irritable bowel syndrome. BMC Gastroenterol. 2015;15:43.
Yang, B., Zhou, X., & Lan, C. (2015). Changes of cytokine levels in a mouse model of post-infectious irritable bowel syndrome. BMC Gastroenterology, 15, 43. https://doi.org/10.1186/s12876-015-0272-8
Yang B, Zhou X, Lan C. Changes of Cytokine Levels in a Mouse Model of Post-infectious Irritable Bowel Syndrome. BMC Gastroenterol. 2015 Apr 1;15:43. PubMed PMID: 25886744.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes of cytokine levels in a mouse model of post-infectious irritable bowel syndrome. AU - Yang,Bo, AU - Zhou,Xuchun, AU - Lan,Cheng, Y1 - 2015/04/01/ PY - 2014/03/05/received PY - 2015/03/20/accepted PY - 2015/4/19/entrez PY - 2015/4/19/pubmed PY - 2016/2/2/medline SP - 43 EP - 43 JF - BMC gastroenterology JO - BMC Gastroenterol VL - 15 N2 - BACKGROUND: Irritable bowel syndrome (IBS) is a highly prevalent functional gastrointestinal disorder. Post-infectious IBS (PI-IBS) is caused by an acute gastrointestinal infection preceding the onset of symptoms. However, the pathophysiology of PI-IBS is not clear, and the purpose of this study was to investigate the probable immune mechanisms of PI-IBS. METHODS: C57BL/6 mice were randomly assigned to either an infection group or a control group. Mice in the infection group were infected with Trichinella spiralis to establish a model of PI-IBS (500 Trichinella), while control mice received only salt solution. Visceral sensitivity of colorectal distention in mice was evaluated by abdominal withdrawal reflex scores and intestinal inflammation was assessed using hematoxylin-eosin staining; at day 56 post-infection, the mRNA and protein levels of specific cytokines in the gut segments were detected using reverse-transcription polymerase chain reaction and enzyme-linked immunoabsorbent assay. RESULTS: Levels of interferon γ and interleukin (IL)-17 in the PI-IBS group were significantly increased in the duodenum and ileum, and IL-10 was decreased in the jejunum, ileum, and colon compared with control mice. However, the expression level of IL-1β was not significantly different between the two groups. CONCLUSIONS: The present study suggests that the local low-grade inflammation and immune activation that are an important component of the pathophysiology of PI-IBS are primarily induced and maintained by specific cytokines. SN - 1471-230X UR - https://www.unboundmedicine.com/medline/citation/25886744/Changes_of_cytokine_levels_in_a_mouse_model_of_post_infectious_irritable_bowel_syndrome_ DB - PRIME DP - Unbound Medicine ER -