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Antioxidant, α-glucosidase inhibitory activity and sub-chronic toxicity of Derris reticulata extract: its antidiabetic potential.
BMC Complement Altern Med. 2015 Feb 27; 15:35.BC

Abstract

BACKGROUND

Antidiabetic activity of Derris reticulata extract on alloxan-induced diabetic rats has been reported. The extract was found to lower blood glucose and inhibit intestinal glucose absorption. The aim of this study was to further investigate mechanisms underlying the antihyperglycemic activity of D. reticulata extract in vitro.

METHODS

The aqueous extract was obtained from D. reticulata stem. Phytochemical screening, total phenolic, and flavanoid contents were examined. ABTS and DPPH scavenging assays, and FRAP method were used to determine in vitro antioxidant activities. Measurement of cell viability on alloxan-induced cellular damage was performed in the insulin-secreting RINm5F cells by MTT assay. The effects of the extract on α-glucosidase activity and insulin release were studied. In addition, sub-chronic toxicity test in rats was also conducted.

RESULTS

The results revealed that the extract, which consisted of terpenoids, saponins, tannins and flavonoids, possessed moderate radical scavenging activities. Pre-treatment of RINm5F cells with the extract was also found to exert moderate, but significant, in vitro protection against alloxan, an oxidative stress producing agent. Unlike glibenclamide, the extract did not stimulate insulin secretion. However, the extract was found to inhibit α-glucosidase activity similar to acarbose. It was found that in sub-chronic toxicity studies D. reticulata extract did not cause mortality or produce any remarkable haematological, biochemical and histopathological adverse effects in rats.

CONCLUSIONS

The data suggest that the possible mechanisms underlying antihyperglycemic activity of D. reticulata extract are cytoprotective effect on pancreatic cells, presumably by its antioxidant activity, and inhibition of α-glucosidase. Sub-chronic toxicity study also provides scientific evidence to corroborate the safety of this plant as an alternative antidiabetic agent.

Authors+Show Affiliations

School of Pharmacology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand. pakarang@g.swu.ac.th. Current address: Division of Health Promotion, Faculty of Health Science, Srinakharinwirot University, Ongkharak, Nakhon-Nayok, 26120, Thailand. pakarang@g.swu.ac.th.School of Anatomy, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand. oratai@g.sut.ac.th.School of Pharmacology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand. nuannoi@sut.ac.th.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25887793

Citation

Kumkrai, Pakarang, et al. "Antioxidant, Α-glucosidase Inhibitory Activity and Sub-chronic Toxicity of Derris Reticulata Extract: Its Antidiabetic Potential." BMC Complementary and Alternative Medicine, vol. 15, 2015, p. 35.
Kumkrai P, Weeranantanapan O, Chudapongse N. Antioxidant, α-glucosidase inhibitory activity and sub-chronic toxicity of Derris reticulata extract: its antidiabetic potential. BMC Complement Altern Med. 2015;15:35.
Kumkrai, P., Weeranantanapan, O., & Chudapongse, N. (2015). Antioxidant, α-glucosidase inhibitory activity and sub-chronic toxicity of Derris reticulata extract: its antidiabetic potential. BMC Complementary and Alternative Medicine, 15, 35. https://doi.org/10.1186/s12906-015-0552-4
Kumkrai P, Weeranantanapan O, Chudapongse N. Antioxidant, Α-glucosidase Inhibitory Activity and Sub-chronic Toxicity of Derris Reticulata Extract: Its Antidiabetic Potential. BMC Complement Altern Med. 2015 Feb 27;15:35. PubMed PMID: 25887793.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant, α-glucosidase inhibitory activity and sub-chronic toxicity of Derris reticulata extract: its antidiabetic potential. AU - Kumkrai,Pakarang, AU - Weeranantanapan,Oratai, AU - Chudapongse,Nuannoi, Y1 - 2015/02/27/ PY - 2014/10/21/received PY - 2015/02/13/accepted PY - 2015/4/19/entrez PY - 2015/4/19/pubmed PY - 2015/11/11/medline SP - 35 EP - 35 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 15 N2 - BACKGROUND: Antidiabetic activity of Derris reticulata extract on alloxan-induced diabetic rats has been reported. The extract was found to lower blood glucose and inhibit intestinal glucose absorption. The aim of this study was to further investigate mechanisms underlying the antihyperglycemic activity of D. reticulata extract in vitro. METHODS: The aqueous extract was obtained from D. reticulata stem. Phytochemical screening, total phenolic, and flavanoid contents were examined. ABTS and DPPH scavenging assays, and FRAP method were used to determine in vitro antioxidant activities. Measurement of cell viability on alloxan-induced cellular damage was performed in the insulin-secreting RINm5F cells by MTT assay. The effects of the extract on α-glucosidase activity and insulin release were studied. In addition, sub-chronic toxicity test in rats was also conducted. RESULTS: The results revealed that the extract, which consisted of terpenoids, saponins, tannins and flavonoids, possessed moderate radical scavenging activities. Pre-treatment of RINm5F cells with the extract was also found to exert moderate, but significant, in vitro protection against alloxan, an oxidative stress producing agent. Unlike glibenclamide, the extract did not stimulate insulin secretion. However, the extract was found to inhibit α-glucosidase activity similar to acarbose. It was found that in sub-chronic toxicity studies D. reticulata extract did not cause mortality or produce any remarkable haematological, biochemical and histopathological adverse effects in rats. CONCLUSIONS: The data suggest that the possible mechanisms underlying antihyperglycemic activity of D. reticulata extract are cytoprotective effect on pancreatic cells, presumably by its antioxidant activity, and inhibition of α-glucosidase. Sub-chronic toxicity study also provides scientific evidence to corroborate the safety of this plant as an alternative antidiabetic agent. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/25887793/Antioxidant_α_glucosidase_inhibitory_activity_and_sub_chronic_toxicity_of_Derris_reticulata_extract:_its_antidiabetic_potential_ DB - PRIME DP - Unbound Medicine ER -