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VRN-1 gene- associated prerequisites of spring growth habit in wild tetraploid wheat T. dicoccoides and the diploid A genome species.
BMC Plant Biol. 2015 Mar 31; 15:94.BP

Abstract

BACKGROUND

In order to clarify the origin of spring growth habit in modern domesticated wheat, allelic variability of the VRN-1 gene was investigated in a wide set of accessions of the wild tetraploid species Triticum dicoccoides (BBAA), together with diploid species T. monococcum, T. boeoticum and T. urartu, presumable donors of the A genome to polyploid wheats.

RESULTS

No significant variation was found at the VRN-B1 locus of T. dicoccoides, whereas at VRN-A1 a number of previously described alleles were found with small deletions in the promoter (VRN-A1b, VRN-A1d) or a large deletion in the first (1st) intron (VRN-A1L). The diploid A genome species were characterized by their own set of VRN-1 alleles including previously described VRN-A1f and VRN-A1h alleles with deletions in the promoter region and the VRN-A1ins allele containing a 0.5 kb insertion in the 1st intron. Based on the CAPS screening data, alleles VRN-A1f and VRN-A1ins were species-specific for T. monococcum, while allele VRN-A1h was specific for T. boeoticum. Different indels were revealed in both the promoter and 1(st) intron of the recessive VRN-A1u allele providing specific identification of T. urartu, the proposed donor of the A genome to modern wheat. We found that alleles VRN-A1b and VRN-A1h, previously described as dominant, have either no or weak association with spring growth habit, while in some diploid accessions this habit was associated with the recessive VRN-A1 allele.

CONCLUSIONS

Spring growth habit in diploid wheats was only partially associated with indels in regulatory regions of the VRN-1 gene. An exception is T. monococcum where dominant mutations in both the promoter region and, especially, the 1st intron were selected during domestication resulting in a greater variety of spring forms. The wild tetraploid T. dicoccoides had a distinct set of VRN-A1 alleles compared to the diploids in this study, indicating an independent origin of spring tetraploid forms that likely occurred after combining of diploid genomes. These alleles were subsequently inherited by cultivated polyploid (tetraploid and hexaploid) descendants.

Authors+Show Affiliations

Institute of Cytology and Genetics, Lavrentiev ave. 10, Novosibirsk, 630090, Russia. atos@bionet.nsc.ru.Institute of Cytology and Genetics, Lavrentiev ave. 10, Novosibirsk, 630090, Russia. pushpandzhali@mail.ru.Institute of Cytology and Genetics, Lavrentiev ave. 10, Novosibirsk, 630090, Russia. salina@bionet.nsc.ru.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25888295

Citation

Shcherban, Andrey B., et al. "VRN-1 Gene- Associated Prerequisites of Spring Growth Habit in Wild Tetraploid Wheat T. Dicoccoides and the Diploid a Genome Species." BMC Plant Biology, vol. 15, 2015, p. 94.
Shcherban AB, Strygina KV, Salina EA. VRN-1 gene- associated prerequisites of spring growth habit in wild tetraploid wheat T. dicoccoides and the diploid A genome species. BMC Plant Biol. 2015;15:94.
Shcherban, A. B., Strygina, K. V., & Salina, E. A. (2015). VRN-1 gene- associated prerequisites of spring growth habit in wild tetraploid wheat T. dicoccoides and the diploid A genome species. BMC Plant Biology, 15, 94. https://doi.org/10.1186/s12870-015-0473-x
Shcherban AB, Strygina KV, Salina EA. VRN-1 Gene- Associated Prerequisites of Spring Growth Habit in Wild Tetraploid Wheat T. Dicoccoides and the Diploid a Genome Species. BMC Plant Biol. 2015 Mar 31;15:94. PubMed PMID: 25888295.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - VRN-1 gene- associated prerequisites of spring growth habit in wild tetraploid wheat T. dicoccoides and the diploid A genome species. AU - Shcherban,Andrey B, AU - Strygina,Kseniya V, AU - Salina,Elena A, Y1 - 2015/03/31/ PY - 2014/12/05/received PY - 2015/03/16/accepted PY - 2015/4/19/entrez PY - 2015/4/19/pubmed PY - 2016/5/18/medline SP - 94 EP - 94 JF - BMC plant biology JO - BMC Plant Biol VL - 15 N2 - BACKGROUND: In order to clarify the origin of spring growth habit in modern domesticated wheat, allelic variability of the VRN-1 gene was investigated in a wide set of accessions of the wild tetraploid species Triticum dicoccoides (BBAA), together with diploid species T. monococcum, T. boeoticum and T. urartu, presumable donors of the A genome to polyploid wheats. RESULTS: No significant variation was found at the VRN-B1 locus of T. dicoccoides, whereas at VRN-A1 a number of previously described alleles were found with small deletions in the promoter (VRN-A1b, VRN-A1d) or a large deletion in the first (1st) intron (VRN-A1L). The diploid A genome species were characterized by their own set of VRN-1 alleles including previously described VRN-A1f and VRN-A1h alleles with deletions in the promoter region and the VRN-A1ins allele containing a 0.5 kb insertion in the 1st intron. Based on the CAPS screening data, alleles VRN-A1f and VRN-A1ins were species-specific for T. monococcum, while allele VRN-A1h was specific for T. boeoticum. Different indels were revealed in both the promoter and 1(st) intron of the recessive VRN-A1u allele providing specific identification of T. urartu, the proposed donor of the A genome to modern wheat. We found that alleles VRN-A1b and VRN-A1h, previously described as dominant, have either no or weak association with spring growth habit, while in some diploid accessions this habit was associated with the recessive VRN-A1 allele. CONCLUSIONS: Spring growth habit in diploid wheats was only partially associated with indels in regulatory regions of the VRN-1 gene. An exception is T. monococcum where dominant mutations in both the promoter region and, especially, the 1st intron were selected during domestication resulting in a greater variety of spring forms. The wild tetraploid T. dicoccoides had a distinct set of VRN-A1 alleles compared to the diploids in this study, indicating an independent origin of spring tetraploid forms that likely occurred after combining of diploid genomes. These alleles were subsequently inherited by cultivated polyploid (tetraploid and hexaploid) descendants. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/25888295/VRN_1_gene__associated_prerequisites_of_spring_growth_habit_in_wild_tetraploid_wheat_T__dicoccoides_and_the_diploid_A_genome_species_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-015-0473-x DB - PRIME DP - Unbound Medicine ER -