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Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China.
Parasit Vectors. 2015 Feb 19; 8:108.PV

Abstract

BACKGROUND

Anaplasmosis is caused by obligate intracellular bacteria in the genus Anaplasma. These bacterial pathogens are transmitted by ticks and impact both human and animal health. This study was conducted to determine the prevalence and molecular characterization of Anaplasma spp. in ruminants sampled in Xinjiang, northwest China.

METHODS

A survey was performed in August 2012 in rural areas of six counties in Xinjiang province. A total of 250 blood samples from ruminants were collected and tested for the presence of Anaplasma spp. by PCR. Positive samples were genetically characterized based on the 16S rRNA and msp4 genes.

RESULTS

The results showed a high prevalence of Anaplasma spp. in ruminants, with at least three different Anaplasma species detected (A. phagocytophilum, A. bovis and A. ovis). The mean prevalence of single infection with each species was 17.6% (A. phagocytophilum), 4.8% (A. bovis) and 40.5% (A. ovis). Coinfection occurred in 20 (8.0%) animals. Phylogenetic analysis of the 16S rRNA gene of A. bovis and A. phagocytophilum revealed a higher degree of genetic diversity for the latter. The results for A. ovis showed genotypic variation among geographic regions in China. In addition, a closely related isolate to the canine pathogen A. platys was identified in ruminants.

CONCLUSIONS

This survey revealed a high prevalence of Anaplasma sp. infections in sheep and cattle in the northwestern border regions of China, indicating the potential risk of transboundary disease.

Authors+Show Affiliations

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China. yangjifei@caas.cn.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China. youquan-li@163.com.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China. lvrilzj@hotmail.com.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China. liujunlong@caas.cn.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China. niuqingli@163.com.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China. haioufei20@yahoo.com.cn.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China. chenze_1215@hotmail.com.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China. guanguiquan2002@hotmail.com.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China. luojianxun@caas.cn.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China. yinhong@caas.cn. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, P. R. China. yinhong@caas.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25889906

Citation

Yang, Jifei, et al. "Molecular Detection and Characterization of Anaplasma Spp. in Sheep and Cattle From Xinjiang, Northwest China." Parasites & Vectors, vol. 8, 2015, p. 108.
Yang J, Li Y, Liu Z, et al. Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China. Parasit Vectors. 2015;8:108.
Yang, J., Li, Y., Liu, Z., Liu, J., Niu, Q., Ren, Q., Chen, Z., Guan, G., Luo, J., & Yin, H. (2015). Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China. Parasites & Vectors, 8, 108. https://doi.org/10.1186/s13071-015-0727-3
Yang J, et al. Molecular Detection and Characterization of Anaplasma Spp. in Sheep and Cattle From Xinjiang, Northwest China. Parasit Vectors. 2015 Feb 19;8:108. PubMed PMID: 25889906.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China. AU - Yang,Jifei, AU - Li,Youquan, AU - Liu,Zhijie, AU - Liu,Junlong, AU - Niu,Qingli, AU - Ren,Qiaoyun, AU - Chen,Ze, AU - Guan,Guiquan, AU - Luo,Jianxun, AU - Yin,Hong, Y1 - 2015/02/19/ PY - 2014/08/27/received PY - 2015/02/09/accepted PY - 2015/4/19/entrez PY - 2015/4/19/pubmed PY - 2016/1/5/medline SP - 108 EP - 108 JF - Parasites & vectors JO - Parasit Vectors VL - 8 N2 - BACKGROUND: Anaplasmosis is caused by obligate intracellular bacteria in the genus Anaplasma. These bacterial pathogens are transmitted by ticks and impact both human and animal health. This study was conducted to determine the prevalence and molecular characterization of Anaplasma spp. in ruminants sampled in Xinjiang, northwest China. METHODS: A survey was performed in August 2012 in rural areas of six counties in Xinjiang province. A total of 250 blood samples from ruminants were collected and tested for the presence of Anaplasma spp. by PCR. Positive samples were genetically characterized based on the 16S rRNA and msp4 genes. RESULTS: The results showed a high prevalence of Anaplasma spp. in ruminants, with at least three different Anaplasma species detected (A. phagocytophilum, A. bovis and A. ovis). The mean prevalence of single infection with each species was 17.6% (A. phagocytophilum), 4.8% (A. bovis) and 40.5% (A. ovis). Coinfection occurred in 20 (8.0%) animals. Phylogenetic analysis of the 16S rRNA gene of A. bovis and A. phagocytophilum revealed a higher degree of genetic diversity for the latter. The results for A. ovis showed genotypic variation among geographic regions in China. In addition, a closely related isolate to the canine pathogen A. platys was identified in ruminants. CONCLUSIONS: This survey revealed a high prevalence of Anaplasma sp. infections in sheep and cattle in the northwestern border regions of China, indicating the potential risk of transboundary disease. SN - 1756-3305 UR - https://www.unboundmedicine.com/medline/citation/25889906/Molecular_detection_and_characterization_of_Anaplasma_spp__in_sheep_and_cattle_from_Xinjiang_northwest_China_ L2 - https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-015-0727-3 DB - PRIME DP - Unbound Medicine ER -