Tags

Type your tag names separated by a space and hit enter

Serum strain-specific or cross-reactive neuraminidase inhibiting antibodies against pandemic А/California/07/2009(H1N1) influenza in healthy volunteers.
BMC Res Notes. 2015 Apr 10; 8:136.BR

Abstract

BACKGROUND

Pre-existing antibodies to influenza virus neuraminidase may provide protection against infection influenza viruses containing novel hemagglutinin (HA). The aim of our study was to evaluate serum neuraminidase-inhibiting (NI) antibodies against А/California/07/2009(H1N1) [H1N1/2009pdm] and А/New Caledonia/20/1999(H1N1) [H1N1/1999] influenza viruses in relation with the age of participants and hemagglutination-inhibition (HI) antibody levels. Anti-H1N1/2009pdm neuraminidase and anti-H1N1/1999 neuraminidase antibody levels were measured in total 219 serum samples from Russian healthy peoples of various ages examined before and a year after pandemic strain appearance. We adjusted peroxidase-linked lectin micro-procedure to measure NI antibody titers using the reassortant A/H7N1 influenza viruses based on A/equine/Prague/1/56(H7N7). Also, HI antibody titers were estimated against H1N1/2009pdm, H1N1/1999 and a panel of seasonal A/H1N1 influenza viruses.

RESULTS

In sera samples collected during the fall of 2010, mean titers of specific HI and NI antibodies to H1N1/2009pdm were 2-2.1 times lower than antibody levels against H1N1/1999. Of the 163 individuals examined, 58 (35.6%) had NI anti-H1N1/2009pdm antibody titers > 1:20, compared to 93 (57.1%) who had NI anti-H1N1/1999 antibody titers > 1:20. There were low correlations between HI and NI antibody levels against either H1N1/1999 or H1N1/2009pdm in the same serum samples. The 24 adults born between 1957 and 1977 expressed very low levels of NI antibodies to A/H1N1 influenza viruses. Persons with low HI anti-H1N1/2009pdm titers but positive to seasonal A/H1N1 demonstrated significantly higher NI anti-A/H1N1 antibody titers than unexposed subjects. In 2005 cross-reactive NI anti-H1N1/2009pdm antibody titers > 1:20 were detected among 7.1% of young people.

CONCLUSIONS

Our study confirmed that contact with seasonal influenza viruses may have contributed to generating the cross-reacting anti-H1N1/2009pdm NI antibodies which were detected in the sera of 18-20 years old people examined before the pandemic virus active circulation. The lowest levels of antibodies to the neuraminidase of N1 subtype were in the group of participants born during the circulation of influenza A/H2N2 or A/H3N2 viruses. The low correlation between HI and NI antibody titers suggests that NI antibody detection can be used as an additional test to evaluate the immune response after influenza infections or immunizations.

Authors+Show Affiliations

Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov's Street, 12, Saint Petersburg, 197376, Russian Federation. desheva@mail.ru.Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov's Street, 12, Saint Petersburg, 197376, Russian Federation. smolonogina@mail.ru.Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov's Street, 12, Saint Petersburg, 197376, Russian Federation. sveta.donina@gmail.com.Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov's Street, 12, Saint Petersburg, 197376, Russian Federation. vaccine@mail.ru.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25889924

Citation

Desheva, Yulia A., et al. "Serum Strain-specific or Cross-reactive Neuraminidase Inhibiting Antibodies Against Pandemic А/California/07/2009(H1N1) Influenza in Healthy Volunteers." BMC Research Notes, vol. 8, 2015, p. 136.
Desheva YA, Smolonogina TA, Donina SA, et al. Serum strain-specific or cross-reactive neuraminidase inhibiting antibodies against pandemic А/California/07/2009(H1N1) influenza in healthy volunteers. BMC Res Notes. 2015;8:136.
Desheva, Y. A., Smolonogina, T. A., Donina, S. A., & Rudenko, L. G. (2015). Serum strain-specific or cross-reactive neuraminidase inhibiting antibodies against pandemic А/California/07/2009(H1N1) influenza in healthy volunteers. BMC Research Notes, 8, 136. https://doi.org/10.1186/s13104-015-1086-z
Desheva YA, et al. Serum Strain-specific or Cross-reactive Neuraminidase Inhibiting Antibodies Against Pandemic А/California/07/2009(H1N1) Influenza in Healthy Volunteers. BMC Res Notes. 2015 Apr 10;8:136. PubMed PMID: 25889924.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serum strain-specific or cross-reactive neuraminidase inhibiting antibodies against pandemic А/California/07/2009(H1N1) influenza in healthy volunteers. AU - Desheva,Yulia A, AU - Smolonogina,Tatiana A, AU - Donina,Svetlana A, AU - Rudenko,Larisa G, Y1 - 2015/04/10/ PY - 2014/12/06/received PY - 2015/03/23/accepted PY - 2015/4/19/entrez PY - 2015/4/19/pubmed PY - 2016/1/7/medline SP - 136 EP - 136 JF - BMC research notes JO - BMC Res Notes VL - 8 N2 - BACKGROUND: Pre-existing antibodies to influenza virus neuraminidase may provide protection against infection influenza viruses containing novel hemagglutinin (HA). The aim of our study was to evaluate serum neuraminidase-inhibiting (NI) antibodies against А/California/07/2009(H1N1) [H1N1/2009pdm] and А/New Caledonia/20/1999(H1N1) [H1N1/1999] influenza viruses in relation with the age of participants and hemagglutination-inhibition (HI) antibody levels. Anti-H1N1/2009pdm neuraminidase and anti-H1N1/1999 neuraminidase antibody levels were measured in total 219 serum samples from Russian healthy peoples of various ages examined before and a year after pandemic strain appearance. We adjusted peroxidase-linked lectin micro-procedure to measure NI antibody titers using the reassortant A/H7N1 influenza viruses based on A/equine/Prague/1/56(H7N7). Also, HI antibody titers were estimated against H1N1/2009pdm, H1N1/1999 and a panel of seasonal A/H1N1 influenza viruses. RESULTS: In sera samples collected during the fall of 2010, mean titers of specific HI and NI antibodies to H1N1/2009pdm were 2-2.1 times lower than antibody levels against H1N1/1999. Of the 163 individuals examined, 58 (35.6%) had NI anti-H1N1/2009pdm antibody titers > 1:20, compared to 93 (57.1%) who had NI anti-H1N1/1999 antibody titers > 1:20. There were low correlations between HI and NI antibody levels against either H1N1/1999 or H1N1/2009pdm in the same serum samples. The 24 adults born between 1957 and 1977 expressed very low levels of NI antibodies to A/H1N1 influenza viruses. Persons with low HI anti-H1N1/2009pdm titers but positive to seasonal A/H1N1 demonstrated significantly higher NI anti-A/H1N1 antibody titers than unexposed subjects. In 2005 cross-reactive NI anti-H1N1/2009pdm antibody titers > 1:20 were detected among 7.1% of young people. CONCLUSIONS: Our study confirmed that contact with seasonal influenza viruses may have contributed to generating the cross-reacting anti-H1N1/2009pdm NI antibodies which were detected in the sera of 18-20 years old people examined before the pandemic virus active circulation. The lowest levels of antibodies to the neuraminidase of N1 subtype were in the group of participants born during the circulation of influenza A/H2N2 or A/H3N2 viruses. The low correlation between HI and NI antibody titers suggests that NI antibody detection can be used as an additional test to evaluate the immune response after influenza infections or immunizations. SN - 1756-0500 UR - https://www.unboundmedicine.com/medline/citation/25889924/Serum_strain_specific_or_cross_reactive_neuraminidase_inhibiting_antibodies_against_pandemic_А/California/07/2009_H1N1__influenza_in_healthy_volunteers_ L2 - https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-015-1086-z DB - PRIME DP - Unbound Medicine ER -