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Lipopolysaccharide preconditioning of adipose-derived stem cells improves liver-regenerating activity of the secretome.
Stem Cell Res Ther 2015; 6:75SC

Abstract

INTRODUCTION

Growing recognition of paracrine mechanisms in stem cell plasticity has resulted in considerable interest in stem cell-derived secretome. The aim of this study was to investigate the effects of lipopolysaccharide (LPS) preconditioning on the composition and hepatic regenerative activity of adipose-derived stem cell (ASC) secretome.

METHODS

Conditioned medium (CM) and LPS-CM were obtained after culturing human ASCs without or with low-dose LPS (0.5 ng/mL) for 24 hours. Untreated and thioacetamide-treated mouse AML12 hepatocytes were incubated for 24 hours with the control medium, LPS (0.5 ng/mL), CM, and LPS-CM and then cell viabilities were compared. CM and LPS-CM were also intravenously administered to partially hepatectomized mice, and their effects on liver regeneration were assessed by using liver weight measurements, immunohistochemistry, and Western blotting.

RESULTS

In the in vitro experiments, LPS preconditioning of ASCs enhanced the mRNA expression levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), hepatocyte growth factor, and vascular endothelial growth factor, which evoke inflammatory response or liver regeneration. LPS-CM significantly promoted thioacetamide-damaged AML12 cell viability compared with CM-incubated cells and the control cells (77%, 69%, and 65% P<0.05). In the in vivo experiment, LPS-CM infusion into the partially hepatectomized mice significantly reduced serum IL-6 and TNF-α levels compared with the other groups (P<0.05) on days 1 and 2 after partial hepatectomy. Moreover, LPS-CM infusion enhanced liver regeneration (based on the liver weight changes at day 7 after partial hepatectomy, 3.73% versus 3.22% in the CM group; P<0.05) and significantly reduced the elevated serum levels of aspartate transaminase and alanine transaminase (at day 1, P<0.05).

CONCLUSIONS

Our results suggest that LPS preconditioning effectively stimulates ASCs to produce the secretome beneficial to hepatic regeneration. Thus, optimizing ASC secretome profile by LPS preconditioning could be a promising approach to treat liver diseases by using stem cells.

Authors+Show Affiliations

Department of Surgery, Daejeon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Daeheung-dong 520-2, Joong-gu, Daejeon, Republic of Korea. zambo9@catholic.ac.kr.Department of Surgery, Daejeon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Daeheung-dong 520-2, Joong-gu, Daejeon, Republic of Korea. redfox0024@nate.com.Department of Surgery, Daejeon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Daeheung-dong 520-2, Joong-gu, Daejeon, Republic of Korea. luislee@catholic.ac.kr.Department of Surgery, Daejeon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Daeheung-dong 520-2, Joong-gu, Daejeon, Republic of Korea. sejoonkim@hanmail.net.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25890074

Citation

Lee, Sang Chul, et al. "Lipopolysaccharide Preconditioning of Adipose-derived Stem Cells Improves Liver-regenerating Activity of the Secretome." Stem Cell Research & Therapy, vol. 6, 2015, p. 75.
Lee SC, Jeong HJ, Lee SK, et al. Lipopolysaccharide preconditioning of adipose-derived stem cells improves liver-regenerating activity of the secretome. Stem Cell Res Ther. 2015;6:75.
Lee, S. C., Jeong, H. J., Lee, S. K., & Kim, S. J. (2015). Lipopolysaccharide preconditioning of adipose-derived stem cells improves liver-regenerating activity of the secretome. Stem Cell Research & Therapy, 6, p. 75. doi:10.1186/s13287-015-0072-7.
Lee SC, et al. Lipopolysaccharide Preconditioning of Adipose-derived Stem Cells Improves Liver-regenerating Activity of the Secretome. Stem Cell Res Ther. 2015 Apr 14;6:75. PubMed PMID: 25890074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipopolysaccharide preconditioning of adipose-derived stem cells improves liver-regenerating activity of the secretome. AU - Lee,Sang Chul, AU - Jeong,Hye Jin, AU - Lee,Sang Kuon, AU - Kim,Say-June, Y1 - 2015/04/14/ PY - 2014/10/20/received PY - 2015/03/31/accepted PY - 2015/03/26/revised PY - 2015/4/19/entrez PY - 2015/4/19/pubmed PY - 2016/1/30/medline SP - 75 EP - 75 JF - Stem cell research & therapy JO - Stem Cell Res Ther VL - 6 N2 - INTRODUCTION: Growing recognition of paracrine mechanisms in stem cell plasticity has resulted in considerable interest in stem cell-derived secretome. The aim of this study was to investigate the effects of lipopolysaccharide (LPS) preconditioning on the composition and hepatic regenerative activity of adipose-derived stem cell (ASC) secretome. METHODS: Conditioned medium (CM) and LPS-CM were obtained after culturing human ASCs without or with low-dose LPS (0.5 ng/mL) for 24 hours. Untreated and thioacetamide-treated mouse AML12 hepatocytes were incubated for 24 hours with the control medium, LPS (0.5 ng/mL), CM, and LPS-CM and then cell viabilities were compared. CM and LPS-CM were also intravenously administered to partially hepatectomized mice, and their effects on liver regeneration were assessed by using liver weight measurements, immunohistochemistry, and Western blotting. RESULTS: In the in vitro experiments, LPS preconditioning of ASCs enhanced the mRNA expression levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), hepatocyte growth factor, and vascular endothelial growth factor, which evoke inflammatory response or liver regeneration. LPS-CM significantly promoted thioacetamide-damaged AML12 cell viability compared with CM-incubated cells and the control cells (77%, 69%, and 65% P<0.05). In the in vivo experiment, LPS-CM infusion into the partially hepatectomized mice significantly reduced serum IL-6 and TNF-α levels compared with the other groups (P<0.05) on days 1 and 2 after partial hepatectomy. Moreover, LPS-CM infusion enhanced liver regeneration (based on the liver weight changes at day 7 after partial hepatectomy, 3.73% versus 3.22% in the CM group; P<0.05) and significantly reduced the elevated serum levels of aspartate transaminase and alanine transaminase (at day 1, P<0.05). CONCLUSIONS: Our results suggest that LPS preconditioning effectively stimulates ASCs to produce the secretome beneficial to hepatic regeneration. Thus, optimizing ASC secretome profile by LPS preconditioning could be a promising approach to treat liver diseases by using stem cells. SN - 1757-6512 UR - https://www.unboundmedicine.com/medline/citation/25890074/Lipopolysaccharide_preconditioning_of_adipose_derived_stem_cells_improves_liver_regenerating_activity_of_the_secretome_ L2 - https://stemcellres.biomedcentral.com/articles/10.1186/s13287-015-0072-7 DB - PRIME DP - Unbound Medicine ER -