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Saponins from Panax japonicus attenuate D-galactose-induced cognitive impairment through its anti-oxidative and anti-apoptotic effects in rats.
J Pharm Pharmacol. 2015 Sep; 67(9):1284-96.JP

Abstract

OBJECTIVE

To investigate the neuroprotective effects of saponins from Panax japonicus (SPJ) on D-galactose (D-gal)-induced brain ageing, and further explore the underlying mechanisms.

METHODS

SPJ were analysed using high-pressure liquid chromatography. Male Wistar rats weighing 200 ± 20 g were randomly divided into four groups: control group (saline), D-gal-treated group (400 mg/kg, subcutaneously), D-gal + SPJ groups (50, 100 and 200 mg/kg, orally) and vitamin E group (100 mg/kg). Rats were injected corresponding drugs once daily for 8 weeks. Neuroprotective effects of SPJ were evaluated by Morris water maze, histopathological observations, biochemical assays, western blot analysis and quantitative real-time polymerase chain reaction (PCR) analysis in vivo as well as reactive oxygen species (ROS) measurement and apoptosis assay in vitro.

KEY FINDINGS

Our present study showed that D-gal had a neurotoxic effect in rats and in SH-SY5Y cells due to oxidative stress induction, including decreased total anti-oxidant capacity, superoxide dismutase (SOD) and glutathione peroxidase activity, ultimately leading to spatial learning and memory impairment in rats and ROS accumulation in SH-SY5Y cells. SPJ improved spatial learning and memory deficits, attenuated hippocampus histopathological injury and restored impaired anti-oxidative as well as anti-apoptotic capacities in D-gal-induced ageing rats. In addition, SPJ remarkably decreased lipofuscin levels, increased hippocampus nuclear factor erythroid 2-related factor 2 (Nrf2) and silent mating type information regulation 2 homologue (SIRT1) protein levels and anti-oxidant genes expression such as manganese superoxide dismutase (Mn-SOD), heme oxygenase (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1) and cysteine ligase catalytic (GCLC) in D-gal-induced brain ageing.

CONCLUSIONS

Our data suggested that D-gal induced multiple molecular and functional changes in brain similar to natural ageing process. SPJ protected brain from D-gal-induced neuronal injury through decreasing oxidative stress and apoptosis, and ultimately improving cognitive performance in D-gal-induced brain ageing. It is possibly related to Nrf2 and SIRT1-mediated anti-oxidant signalling pathways.

Authors+Show Affiliations

College of Medical Science, Three Gorges University.College of Medical Science, Three Gorges University.College of Medical Science, Three Gorges University.College of Medical Science, Three Gorges University.Three Gorges Career Technical College of Nursing.College of Medical Science, Three Gorges University.College of Medical Science, Three Gorges University.Institute of Molecular Biology, Three Gorges University.Second People's Hospital of China, Three Gorges University.College of Medical Science, Three Gorges University.College of Medical Science, Three Gorges University. Renhe Hospital, Three Gorges University.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25892055

Citation

Wang, Ting, et al. "Saponins From Panax Japonicus Attenuate D-galactose-induced Cognitive Impairment Through Its Anti-oxidative and Anti-apoptotic Effects in Rats." The Journal of Pharmacy and Pharmacology, vol. 67, no. 9, 2015, pp. 1284-96.
Wang T, Di G, Yang L, et al. Saponins from Panax japonicus attenuate D-galactose-induced cognitive impairment through its anti-oxidative and anti-apoptotic effects in rats. J Pharm Pharmacol. 2015;67(9):1284-96.
Wang, T., Di, G., Yang, L., Dun, Y., Sun, Z., Wan, J., Peng, B., Liu, C., Xiong, G., Zhang, C., & Yuan, D. (2015). Saponins from Panax japonicus attenuate D-galactose-induced cognitive impairment through its anti-oxidative and anti-apoptotic effects in rats. The Journal of Pharmacy and Pharmacology, 67(9), 1284-96. https://doi.org/10.1111/jphp.12413
Wang T, et al. Saponins From Panax Japonicus Attenuate D-galactose-induced Cognitive Impairment Through Its Anti-oxidative and Anti-apoptotic Effects in Rats. J Pharm Pharmacol. 2015;67(9):1284-96. PubMed PMID: 25892055.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Saponins from Panax japonicus attenuate D-galactose-induced cognitive impairment through its anti-oxidative and anti-apoptotic effects in rats. AU - Wang,Ting, AU - Di,Guojie, AU - Yang,Li, AU - Dun,Yaoyan, AU - Sun,Zhiwei, AU - Wan,Jingzhi, AU - Peng,Ben, AU - Liu,Chaoqi, AU - Xiong,Guangrun, AU - Zhang,Changcheng, AU - Yuan,Ding, Y1 - 2015/04/18/ PY - 2014/09/10/received PY - 2015/01/18/accepted PY - 2015/4/21/entrez PY - 2015/4/22/pubmed PY - 2016/5/6/medline KW - apoptosis KW - braining ageing KW - oxidative stress KW - saponins from Panax japonicus SP - 1284 EP - 96 JF - The Journal of pharmacy and pharmacology JO - J. Pharm. Pharmacol. VL - 67 IS - 9 N2 - OBJECTIVE: To investigate the neuroprotective effects of saponins from Panax japonicus (SPJ) on D-galactose (D-gal)-induced brain ageing, and further explore the underlying mechanisms. METHODS: SPJ were analysed using high-pressure liquid chromatography. Male Wistar rats weighing 200 ± 20 g were randomly divided into four groups: control group (saline), D-gal-treated group (400 mg/kg, subcutaneously), D-gal + SPJ groups (50, 100 and 200 mg/kg, orally) and vitamin E group (100 mg/kg). Rats were injected corresponding drugs once daily for 8 weeks. Neuroprotective effects of SPJ were evaluated by Morris water maze, histopathological observations, biochemical assays, western blot analysis and quantitative real-time polymerase chain reaction (PCR) analysis in vivo as well as reactive oxygen species (ROS) measurement and apoptosis assay in vitro. KEY FINDINGS: Our present study showed that D-gal had a neurotoxic effect in rats and in SH-SY5Y cells due to oxidative stress induction, including decreased total anti-oxidant capacity, superoxide dismutase (SOD) and glutathione peroxidase activity, ultimately leading to spatial learning and memory impairment in rats and ROS accumulation in SH-SY5Y cells. SPJ improved spatial learning and memory deficits, attenuated hippocampus histopathological injury and restored impaired anti-oxidative as well as anti-apoptotic capacities in D-gal-induced ageing rats. In addition, SPJ remarkably decreased lipofuscin levels, increased hippocampus nuclear factor erythroid 2-related factor 2 (Nrf2) and silent mating type information regulation 2 homologue (SIRT1) protein levels and anti-oxidant genes expression such as manganese superoxide dismutase (Mn-SOD), heme oxygenase (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1) and cysteine ligase catalytic (GCLC) in D-gal-induced brain ageing. CONCLUSIONS: Our data suggested that D-gal induced multiple molecular and functional changes in brain similar to natural ageing process. SPJ protected brain from D-gal-induced neuronal injury through decreasing oxidative stress and apoptosis, and ultimately improving cognitive performance in D-gal-induced brain ageing. It is possibly related to Nrf2 and SIRT1-mediated anti-oxidant signalling pathways. SN - 2042-7158 UR - https://www.unboundmedicine.com/medline/citation/25892055/Saponins_from_Panax_japonicus_attenuate_D_galactose_induced_cognitive_impairment_through_its_anti_oxidative_and_anti_apoptotic_effects_in_rats_ L2 - https://doi.org/10.1111/jphp.12413 DB - PRIME DP - Unbound Medicine ER -